Detection of Human MIA by Western Blot. Western blot shows lysates of Hs 294T human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MIA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2050) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MIA at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
MIA in Human Melanoma. MIA was detected in immersion fixed paraffin-embedded sections of human melanoma using Goat Anti-Human MIA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2050) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
MIA, also named Cartilage-Derived Retinoic Acid-Sensitive Protein
(CD-RAP), is a secreted protein that plays an important role in
melanoma metastasis. It is highly expressed in malignant melanomas and
is associated with tumor progression.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.