Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MIA Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MIA
Gly25-Gln131
Accession # Q16674

Specificity

Detects human MIA in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human MIA Antibody

Detection of Human MIA antibody by Western Blot.

Detection of Human MIA by Western Blot.

Western blot shows lysates of Hs 294T human melanoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MIA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2050) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MIA at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

MIA antibody in Human Melanoma by Immunohistochemistry (IHC-P).

MIA in Human Melanoma.

MIA was detected in immersion fixed paraffin-embedded sections of human melanoma using Goat Anti-Human MIA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2050) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human MIA Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human melanoma

Western Blot

1 µg/mL
Sample: Hs 294T human melanoma cell line

Reviewed Applications

Read 1 review rated 5 using AF2050 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MIA

MIA, also named Cartilage-Derived Retinoic Acid-Sensitive Protein (CD-RAP), is a secreted protein that plays an important role in melanoma metastasis. It is highly expressed in malignant melanomas and is associated with tumor progression.

Long Name

Melanoma Inhibitory Activity

Alternate Names

CD-RAP

Entrez Gene IDs

8190 (Human); 12587 (Mouse)

Gene Symbol

MIA

UniProt

Q16674

Additional MIA Products

Product Documents for Human MIA Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MIA Antibody

For research use only

Citations for Human MIA Antibody

Customer Reviews for Human MIA Antibody (1)

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  • Human MIA Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 05/22/2019
    AF2050 was tested along with MAB20501 in a sandwich assay. Although both antibodies worked as either the capture or detection, AF2050 had significantly higher affinity, which allowed quantification at a lower dilution of samples. The version of the assay we ended up using was AF2050 as both the capture and detection.
    Human MIA Antibody AF2050

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Protocols

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FAQs

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