Human MICL/CLEC12A Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB29461-100
MAB29461-SP
Detection of MICL/CLEC12A in Human Peripheral Blood Granulocytes by Flow Cytometry.
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Human MICL/CLEC12A Antibody Summary

Species Reactivity
Human
Specificity
Detects human MICL/CLEC12A in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2439B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MICL/CLEC12A
Thr67-Ala265
Accession # Q5QGZ9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
3-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of MICL/CLEC12A in Human Peripheral Blood Granulocytes by Flow Cytometry. View Larger

Detection of MICL/CLEC12A in Human Peripheral Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Rabbit Anti-Human MICL/CLEC12A Monoclonal Antibody (Catalog # MAB29461, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry MICL/CLEC12A  in U937 Human Cell Line. View Larger

MICL/CLEC12A in U937 Human Cell Line. MICL/CLEC12A was detected in immersion fixed U937 human histiocytic lymphoma cell line (left panel; positive staining) and Daudi human Burkitt's lymphoma cell line (right panel; negative staining) using Rabbit Anti-Human MICL/CLEC12A Monoclonal Antibody (Catalog # MAB29461) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry MICL/CLEC12A  in Human Spleen. View Larger

MICL/CLEC12A in Human Spleen. MICL/CLEC12A was detected in immersion fixed paraffin-embedded sections of human spleen using Rabbit Anti-Human MICL/CLEC12A Monoclonal Antibody (Catalog # MAB29461) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MICL/CLEC12A

Human MICL (myeloid inhibitory C-type lectin-like receptor; also CLL-1 and KLRL-1) is a 75 kDa member of the group V C-type lectin-like receptor family of molecules. Group V molecules have a lectin-type domain that binds non-sugar ligands. MICL is a 265 amino acid (aa) type II transmembrane (TM) glycoprotein that contains a 200 aa extracellular domain (ECD). Multiple isoforms of MICL are reported. One potentially utilizes an alternate start site that adds 10 aa to the cytoplasmic domain. There is one potential soluble form that lacks the TM segment, and two truncated ECD isoforms. Human MICL ECD is 53%, 65%, and 55% aa identical to the ECD in mouse, dog and cow, respectively.

Long Name
Myeloid Inhibitory C-type Lectin-like Receptor
Entrez Gene IDs
160364 (Human); 232413 (Mouse)
Alternate Names
CD371; CLEC12A; CLL1; CLL-1; C-type lectin domain family 12, member A; DCAL-2; MICL

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

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