Human Mind Bomb 2/MIB2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Mind Bomb 2/MIB2 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 739505
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Mind Bomb 2/MIB2
    Ala418-Lys520
    Accession # Q96AX9
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Mind Bomb 2/MIB2 by Western Blot. Western blot shows lysates of U266 human myeloma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Mind Bomb 2/MIB2 Monoclonal Antibody (Catalog # MAB72891) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Mind Bomb 2/MIB2 at approximately 103-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mind Bomb 2/MIB2
MIB-2 (Mind Bomb-2), also called skeletrophin, is a widely expressed, 1013 amino acid (aa) cytoplasmic phosphoprotein that is an E3 ubiquitin ligase for Notch ligands such as Jagged-2. It contains two MIB/HERC2 domains surrounding a ZZ-type zinc finger (aa 59-285), an ankyrin repeat domain (aa 522-856), and two RING-type zinc fingers that have E3 ubiquitin ligase activity (aa 890-1002). If short gaps in the protein sequence are ignored, human MIB-2 (aa 418-520) shares 95% and 92% aa identity with mouse and rat MIB-2, respectively. Eight alternately spliced isoforms of 672-1009 aa lack various sequences, but all contain aa 418-520. MIB-2 has been detected as a 70 kDa form in skeletal muscle, binds actin, and is downregulated in malignant melanoma.
  • Long Name:
    Mindbomb Homolog 2
  • Entrez Gene IDs:
    142678 (Human); 76580 (Mouse)
  • Alternate Names:
    E3 ubiquitin-protein ligase MIB2; EC 6.3.2; EC 6.3.2.-; EC 6.3.2.13; FLJ20648; FLJ41406; MIB2; Mind Bomb 2; Mind bomb homolog 2; Mind Bomb-2; mindbomb homolog 2 (Drosophila); Novel zinc finger protein; Novelzin; Putative NF-kappa-B-activating protein 002N; SKD; Skeletrophin; Zinc finger ZZ type with ankyrin repeat domain protein 1; ZZ type with ankyrin repeat domain 1; ZZANK1; ZZZ5
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Mouse IgG2B Antibody

Flow MAB0043  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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