Detection of Human Mind Bomb 2/MIB2 by Western Blot. Western blot shows lysates of U266 human myeloma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Mind Bomb 2/MIB2 Monoclonal Antibody (Catalog # MAB72891) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Mind Bomb 2/MIB2 at approximately 103-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mind Bomb 2/MIB2
(Mind Bomb-2), also called skeletrophin, is a widely expressed, 1013 amino acid
(aa) cytoplasmic phosphoprotein that is an E3 ubiquitin ligase for Notch
ligands such as Jagged-2. It contains two MIB/HERC2 domains surrounding a
ZZ-type zinc finger (aa 59-285), an ankyrin repeat domain (aa 522-856), and two
RING-type zinc fingers that have E3 ubiquitin ligase activity (aa 890-1002). If
short gaps in the protein sequence are ignored, human MIB-2 (aa 418-520) shares
95% and 92% aa identity with mouse and rat MIB-2, respectively. Eight
alternately spliced isoforms of 672-1009 aa lack various sequences, but all
contain aa 418-520. MIB-2 has been detected as a 70 kDa form in skeletal
muscle, binds actin, and is downregulated in malignant melanoma.
Mindbomb Homolog 2
Entrez Gene IDs:
142678 (Human); 76580 (Mouse)
E3 ubiquitin-protein ligase MIB2; EC 6.3.2; EC 6.3.2.-; EC 18.104.22.168; FLJ20648; FLJ41406; MIB2; Mind bomb homolog 2; Mind Bomb-2; mindbomb homolog 2 (Drosophila); Novel zinc finger protein; Novelzin; Putative NF-kappa-B-activating protein 002N; SKD; Skeletrophin; Zinc finger ZZ type with ankyrin repeat domain protein 1; ZZ type with ankyrin repeat domain 1; ZZANK1; ZZZ5
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