Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MIS/AMH Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MIS/AMH Propeptide
Leu19-Gln450
Accession # P03971

Specificity

Detects human MIS/AMH Propeptide in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with mature recombinant human MIS is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human MIS/AMH Propeptide Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ovary subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Western Blot

0.1 µg/mL
Sample: Recombinant Human MIS/AMH (Catalog # 1737-MS)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MIS/AMH

MIS, also known as anti-Mullerian Hormone (AMH), belongs to the TGF-beta superfamily. It is synthesized as a 140 kDa homodimeric glycoprotein that undergoes proteolytic cleavage into the bioactive homodimeric 26 kDa carboxy-terminus and the 110 kDa dimeric amino-terminus. After cleavage, the amino-terminal propeptide remains non-covalently associated with the bioactive carboxy-terminal active protein. The amino acid sequence of human and mouse MIS propeptides are 67% identical.

Long Name

Mullerian-inhibiting Substance/Anti-Mullerian Hormone

Alternate Names

AMH

Entrez Gene IDs

268 (Human); 11705 (Mouse)

Gene Symbol

AMH

UniProt

P03971

Additional MIS/AMH Products

Product Documents for Human MIS/AMH Propeptide Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human MIS/AMH Propeptide Antibody

For research use only

Citations for Human MIS/AMH Propeptide Antibody

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Protocols

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FAQs

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