Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MLX Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MLX
Glu197-Tyr298
Accession # EAW60842

Specificity

Detects human MLX in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human MLX Antibody

Detection of Human MLX antibody by Western Blot.

Detection of Human MLX by Western Blot.

Western blot shows nuclear extracts of PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MLX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4186) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MLX at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

MLX in U2OS Human Cell Line.

MLX was detected in immersion fixed U2OS human osteosarcoma cell line using Goat Anti-Human MLX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4186) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human MLX Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed U2OS human osteosarcoma cell line

Western Blot

1 µg/mL
Sample: PC‑3 human prostate cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MLX

MLX (Max-like protein X; also known as transcription factor-like protein 4) is a 30 kDa member of the Max-like bHLHZip family of proteins. It is widely expressed and is found in both cytoplasm and nucleus. MLX is a transcriptional repressor when dimerized with MAD family members and a transcriptional activator when dimerized with MONDO family members. Human MLX is 298 amino acids (aa) in length. It contains one DNA-binding region (aa 76‑87), an HLH domain (aa 127‑192) and a Leu-zipper domain (aa 140‑160). There are three alternate splice short forms. One shows the deletion of aa 15‑68, a second shows deletion of aa 15‑68 and 81‑110, and a third shows a 3 aa substitution for aa 111‑298. Over aa 197‑298, human MLX is 99% identical to mouse and canine MLX.

Long Name

MAX-like Protein X

Alternate Names

BigMax alpha, MAD7, MXD7, TCFL4

Entrez Gene IDs

6945 (Human); 21428 (Mouse); 360631 (Rat)

Gene Symbol

MLX

UniProt

EAW60842

Additional MLX Products

Product Documents for Human MLX Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MLX Antibody

For research use only

Citations for Human MLX Antibody

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Protocols

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