|Detection of Human MLX by Western Blot. Western blot shows nuclear extracts of PC‑3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MLX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4186) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MLX at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
MLX (Max-like protein X; also known as transcription factor-like protein 4) is a 30 kDa member of the Max-like bHLHZip family of proteins. It is widely expressed and is found in both cytoplasm and nucleus. MLX is a transcriptional repressor when dimerized with MAD family members and a transcriptional activator when dimerized with MONDO family members. Human MLX is 298 amino acids (aa) in length. It contains one DNA-binding region (aa 76‑87), an HLH domain (aa 127‑192) and a Leu-zipper domain (aa 140‑160). There are three alternate splice short forms. One shows the deletion of aa 15‑68, a second shows deletion of aa 15‑68 and 81‑110, and a third shows a 3 aa substitution for aa 111‑298. Over aa 197‑298, human MLX is 99% identical to mouse and canine MLX.
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