Human MLX Antibody

Catalog # Availability Size / Price Qty
Detection of Human MLX by Western Blot.
2 Images
Product Details
Citations (1)
Supplemental Products

Human MLX Antibody Summary

Species Reactivity
Detects human MLX in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human MLX
Accession # EAW60842
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
Immersion fixed U2OS human osteosarcoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human MLX antibody by Western Blot. View Larger

Detection of Human MLX by Western Blot. Western blot shows nuclear extracts of PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MLX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4186) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MLX at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry View Larger

MLX in U2OS Human Cell Line. MLX was detected in immersion fixed U2OS human osteosarcoma cell line using Goat Anti-Human MLX Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4186) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MLX

MLX (Max-like protein X; also known as transcription factor-like protein 4) is a 30 kDa member of the Max-like bHLHZip family of proteins. It is widely expressed and is found in both cytoplasm and nucleus. MLX is a transcriptional repressor when dimerized with MAD family members and a transcriptional activator when dimerized with MONDO family members. Human MLX is 298 amino acids (aa) in length. It contains one DNA-binding region (aa 76‑87), an HLH domain (aa 127‑192) and a Leu-zipper domain (aa 140‑160). There are three alternate splice short forms. One shows the deletion of aa 15‑68, a second shows deletion of aa 15‑68 and 81‑110, and a third shows a 3 aa substitution for aa 111‑298. Over aa 197‑298, human MLX is 99% identical to mouse and canine MLX.

Long Name
MAX-like Protein X
Entrez Gene IDs
6945 (Human); 21428 (Mouse); 360631 (Rat)
Alternate Names
BHLHD13; bHLHd13Max-like bHLHZip protein; BigMax alpha; BigMax protein; Class D basic helix-loop-helix protein 13; MAD7; MAX-like protein X; MLX; MXD7; TCFL4; TCFL4Protein BigMax; transcription factor-like 4; Transcription factor-like protein 4

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Citation for Human MLX Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Thioredoxin-interacting protein gene expression via MondoA is rapidly and transiently suppressed during inflammatory responses.
    Authors: Kanari Y, Sato Y, Aoyama S, Muta T
    PLoS ONE, 2013;8(3):e59026.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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