Detects human MMP-12 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse MMP-12 is observed, and less than 2% cross-reactivity with recombinant human (rh) MMP-1 and rhMMP-3 is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human MMP-12 Leu17-Cys470 Accession # P39900
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human Pro‑MMP‑12 by Western Blot.
Western blot shows lysates of human heart tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human MMP‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF917) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Pro‑MMP‑12 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
MMP‑12 in Human Squamous Cell Carcinoma.
MMP‑12 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Goat Anti-Human MMP‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF917) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-12 (macrophage elastase) is found in macrophages and its expression in monocytes can be induced by cytokines such as GM-CSF and CD40 signaling (1). In addition to elastin, MMP-12 can degrade a broad spectrum of substrates, including type IV collagen, fibronectin, laminin, vitronectin, proteoglycans, chondroitin sulfate, myelin basic protein, alpha 1-antitrypsin, and plasminogen. It can also activate MMP-2 and MMP-3. MMP-12 is required for macrophage‑mediated proteolysis and matrix invasion in mice. MMP-12 is proposed to have a direct role in the pathogenesis of aortic aneurysms and in the development of pulmonary emphysema that results from chronic inhalation of cigarette smoke. Structurally, the pro MMP-12 consists of following domains: a pro domain, a catalytic domain containing the zinc-binding site, and a C-terminal hemopexin-like domain. The rhMMP-12 corresponds to the pro form that can be activated by autocatalysis under the conditions described above.
S.D. Shapiro et al. (2004) in Handbook of Proteolytic Enzymes (eds. A.J. Barrett et al.) pp.540 - 544, Academic Press, San Diego.
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