Human MMP-17 Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects human MMP-17 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human MMP-24 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 823511
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human MMP-17
    Pro42-Ala534
    Accession # Q9ULZ9
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization

    Measured by itsability to neutralize Recombinant Human MMP-17 (1.0 μg/mL, Catalog # 7796-MP) cleavage of the fluorogenic peptide substrate Mca-PLAQAV-Dpa-RSSSR-NH2 (10μM, Catalog # ES003). The Neutralization Dose (ND50) is < 9.0 μg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Neutralization of Human MMP‑17 by Human MMP-17 Antibody. The cleavage of Mca‑PLAQAV‑Dpa‑RSSSR‑NH2 (10 μM, Catalog # ES003) by Recombinant Human MMP-17 (1.0 ug/mL, Catalog # 7796-MP) is measured after it has been preincubated with increasing concentrations of Mouse Anti-Human MMP-17 Monoclonal Antibody (Catalog # MAB7796). The ND50 is typically 9.0 μg/mL.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-17
Matrix metalloproteinase 17 (MMP‑17) is a zinc metalloproteinase that is an integral membrane protein (1). It belongs to the membrane‑type MMP (MT‑MMP) subfamily, and is also known as MT4‑MMP. It is associated with the cell membrane by a glycosylphosphatidylinositol (GPI) anchor (1). It has the potential to function as a pro‑tumor necrosis factor‑ alpha  (TNF‑ alpha ) convertase (2). Unlike MMP‑14 (MT1‑MMP), it is a poor activator of pro‑MMP‑2. MMP‑17 is relatively poor at digesting components of the extracellular matrix but does cleave fibrinogen and fibrin (2). MMP‑17 is known to activate the aggrecanase ADAMTS‑4 through proteolysis (3). MMP‑17 is expressed in many tissues with the highest levels seen in the brain, colon, ovary, testis, and kidney papilla (4). It has been detected in several human cancers, including gliomas, prostate carcinomas, and breast carcinomas (5).
  • References:
    1. Itoh, Y. et al. (1999) J. Biol. Chem. 274:34260.
    2. English, W. R. et al. (2000) J. Biol. Chem. 275:14046.
    3. Gao, G. et al. (2004) J. Biol. Chem. 279:10042.
    4. Srichai, M. B. et al. (2011) PLoS ONE 6:e17099.
    5. Sohail, A. et al. (2008) Cancer Metastasis Rev.  27:289.
  • Long Name:
    Matrix Metalloproteinase 17
  • Entrez Gene IDs:
    4326 (Human); 23948 (Mouse); 288626 (Rat)
  • Alternate Names:
    matrix metallopeptidase 17 (membrane-inserted); matrix metalloproteinase 17 (membrane-inserted); matrix metalloproteinase-17; membrane-type matrix metalloproteinase 4; membrane-type-4 matrix metalloproteinase; MMP17; MMP-17; MT4MMP; MT4-MMP; MT-MMP 4; MTMMP4
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FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 35  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 32  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 12  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow , IHC , ICC NL007 10
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 4  
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG Antibody

WB AF007 4
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG2A Antibody

WB , Flow MAB0033  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Mouse IgG2A Antibody

Flow MAB0032  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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