Human MMP-2 Antibody

(9 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects pro/active forms of human MMP-2 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MMP‑9, rhMMP-1, or rhMMP-3 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 36006
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human MMP-2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human MMP‑2 Western Blot Standard (Catalog # WBC025) under non-reducing conditions only
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
MMP‑2 in Human Ovarian Cancer Tissue.

MMP‑2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Mouse Anti-Human MMP‑2 Monoclonal Antibody (Catalog # MAB902) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry
MMP‑2 in Human Ovarian Cancer Tissue.

MMP‑2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Mouse Anti-Human MMP‑2 Monoclonal Antibody (Catalog # MAB902) at 15 µg/mL overnight at 4 °C. Tissue was stained (red) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-2
Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP-2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting;and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites.
  • Long Name:
    Matrix Metalloproteinase 2
  • Entrez Gene IDs:
    4313 (Human); 17390 (Mouse)
  • Alternate Names:
    72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
Applications
Sample Type
  1. The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy.
    Authors: Li MQ, Hou XF, Shao J, Tang CL, Li DJ
    Biol. Reprod., 2010;82(5):968-79.
    Species: Human
    Sample Type: Whole Cells
    Application: Cell-based ELISA
  2. High matrix metalloproteinase production correlates with immune activation and leukocyte migration in leprosy reactional lesions.
    Authors: Teles RM, Teles RB, Amadeu TP, Moura DF, Mendonca-Lima L, Ferreira H, Santos IM, Nery JA, Sarno EN, Sampaio EP
    Infect. Immun., 2010;78(3):1012-21.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  3. Monocyte chemoattractant protein-1 (MCP-1)/CCL2 secreted by hepatic myofibroblasts promotes migration and invasion of human hepatoma cells.
    Authors: Dagouassat M, Suffee N, Hlawaty H, Haddad O, Charni F, Laguillier C, Vassy R, Martin L, Schischmanoff PO, Gattegno L, Oudar O, Sutton A, Charnaux N
    Int. J. Cancer, 2010;126(5):1095-108.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. In vitro secretion of matrix metalloprotease 9 is a prognostic marker in childhood acute lymphoblastic leukemia.
    Authors: Schneider P, Costa O, Legrand E
    Leuk. Res., 2010;34(1):24-31.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Effect of bimatoprost, latanoprost, and unoprostone on matrix metalloproteinases and their inhibitors in human ciliary body smooth muscle cells.
    Authors: Ooi YH, Oh DJ, Rhee DJ
    Invest. Ophthalmol. Vis. Sci., 2009;50(11):5259-65.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. pVHL co-ordinately regulates CXCR4/CXCL12 and MMP2/MMP9 expression in human clear-cell renal cell carcinoma.
    Authors: Struckmann K, Mertz K, Steu S, Storz M, Staller P, Krek W, Schraml P, Moch H
    J. Pathol., 2008;214(4):464-71.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  7. Urinary biomarkers predict brain tumor presence and response to therapy.
    Authors: Smith ER, Zurakowski D, Saad A, Scott RM, Moses MA
    Clin. Cancer Res., 2008;14(8):2378-86.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  8. Role of platelet-derived growth factor and transforming growth factor beta1 the in the regulation of metalloproteinase expressions.
    Authors: Borrelli V, di Marzo L, Sapienza P, Colasanti M, Moroni E, Cavallaro A
    Surgery, 2006;140(3):454-63.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  9. Oxidized low-density lipoproteins stimulate extracellular matrix metalloproteinase Inducer (EMMPRIN) release by coronary smooth muscle cells.
    Authors: Haug C, Lenz C, Diaz F, Bachem MG
    Arterioscler. Thromb. Vasc. Biol., 2004;24(10):1823-9.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
Cell and Tissue Staining Kits
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Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

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Immunohistochemistry Reagents
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VisUCyte Antigen Retrieval Reagent-Universal

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Isotype Controls
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Mouse IgG1 Isotype Control

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Mouse IgG1 Isotype Control

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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

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Donkey Anti-Mouse IgG Biotinylated Antibody

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Mouse IgG HRP-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Goat Anti-Mouse IgG Biotinylated Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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