Human MMP-2 Antibody

(6 citations)
(3 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human MMP-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 50-100% cross-reactivity with recombinant mouse MMP-2 is observed, approximately 15% cross-reactivity with recombinant human (rh) MMP‑7 and less than 5% cross-reactivity with rhMMP‑1, rhMMP‑3, rhMMP‑8, rhMMP‑9, rhMMP‑10, rhMMP‑12, rhMMP‑13, rhMMP-14, rhMMP-15, rhMMP-16, rhMMP-17, rhMMP-19, and rhMMP-24 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human MMP‑2
    Ile34-Cys660
    Accession # P08253
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human MMP‑2 by Western Blot. Western blot shows lysate of U‑118‑MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF902) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP‑2 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
MMP‑2 in Human Ovarian Cancer Tissue. MMP‑2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human MMP‑2 Antigen Affinity-purified Poly­clonal Antibody (Catalog # AF902) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
MMP‑2 in Human Ovary. MMP‑2 was detected in immersion fixed paraffin-embedded sections of human ovarian array using Goat Anti-Human MMP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF902) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human MMP‑2 by Simple WesternTM. Simple Western lane view shows lysates of U‑118‑MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for MMP‑2 at approximately 78 kDa (as indicated) using 10 µg/mL of Goat Anti-Human MMP‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF902) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody
(Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-2

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP-2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting; and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites.

  • Long Name:
    Matrix Metalloproteinase 2
  • Entrez Gene IDs:
    4313 (Human); 17390 (Mouse)
  • Alternate Names:
    72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Humanin, a cytoprotective peptide, is expressed in carotid atherosclerotic [corrected] plaques in humans.
    Authors: Zacharias DG, Kim SG, Massat AE, Bachar AR, Oh YK, Herrmann J, Rodriguez-Porcel M, Cohen P, Lerman LO, Lerman A
    PLoS ONE, 2012;7(2):e31065.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. ErbB2-enhanced invasiveness of H-Ras MCF10A breast cells requires MMP-13 and uPA upregulation via p38 MAPK signaling.
    Authors: Yong HY, Kim IY, Kim JS, Moon A
    Int. J. Oncol., 2010;36(2):501-7.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
  3. Fibroblast-conditioned media promote human sarcoma cell invasion.
    Authors: Bittner JG, Wilson M, Shah MB, Albo D, Feig BW, Wang TN
    Surgery, 2009;145(1):42-7.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008;7(6):2406-14.
    Species: Human
    Sample Type: Serum
    Application: ELISA Microarray Development
  5. Claudin-1 overexpression in melanoma is regulated by PKC and contributes to melanoma cell motility.
    Authors: Leotlela PD, Wade MS, Duray PH, Rhode MJ, Brown HF, Rosenthal DT, Dissanayake SK, Earley R, Indig FE, Nickoloff BJ, Taub DD, Kallioniemi OP, Meltzer P, Morin PJ, Weeraratna AT
    Oncogene, 2007;26(26):3846-56.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  6. Differential expression and activity of matrix metalloproteinases during flow-modulated vein graft remodeling.
    Authors: Berceli SA, Jiang Z, Klingman NV, Pfahnl CL, Abouhamze ZS, Frase CD, Schultz GS, Ozaki CK
    J. Vasc. Surg., 2004;39(5):1084-90.
    Species: Rabbit
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
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ExactaChIP Chromatin Immunoprecipitation Kits
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ExactaChIP Chromatin IP Buffer Panel

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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NL996
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VCTS022
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VCTS021
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VCTS023
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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Average Rating: 4.3 (Based on 3 reviews)

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We have 2 reviews tested in 1 species: Human.
We have 3 reviews tested in 2 applications: ELISA, Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
ELISA Human MMP-2 Antibody AF902
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Very Good
 ELISA Human Anonymous 11/09/2017
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ELISA Human MMP-2 Antibody AF902
ELISA: Human MMP-2 Antibody [AF902]

Summary

ApplicationELISA
Sample TestedSerum and Plasma
SpeciesHuman

Other Experimental Details

Other Experimental DetailsThe polyclonal goat antibody AF902 was used as both capture and detection to build and ELISA measuring MMP-2 in human serum and plasma samples.
Western Blot Human MMP‑2 Antibody AF902
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Excellent
 WB Human Anonymous 02/27/2016
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Western Blot Human MMP‑2 Antibody AF902
Western Blot: Human MMP‑2 Antibody [AF902]

Summary

ApplicationWestern Blot
Sample TestedHuman cell conditioned medium
SpeciesHuman
  Very Good
 WB Anonymous 11/23/2015
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Summary

ApplicationWestern Blot
Sample TestedTrabecular meshwork endothelial

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