Human MMP-2 Antibody

Catalog # Availability Size / Price Qty
AF902
AF902-SP
Human MMP-2 Antibody in Data
4 Images
Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (4)

Human MMP-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MMP-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 50-100% cross-reactivity with recombinant mouse MMP-2 is observed, approximately 15% cross-reactivity with recombinant human (rh) MMP‑7 and less than 5% cross-reactivity with rhMMP‑1, rhMMP‑3, rhMMP‑8, rhMMP‑9, rhMMP‑10, rhMMP‑12, rhMMP‑13, rhMMP-14, rhMMP-15, rhMMP-16, rhMMP-17, rhMMP-19, and rhMMP-24 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP‑2
Ile34-Cys660
Accession # P08253
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑2 (Catalog # 902-MP), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human MMP‑2 antibody by Western Blot. View Larger

Detection of Human MMP‑2 by Western Blot. Western blot shows lysate of U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF902) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP-2 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry MMP-2 antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

MMP‑2 in Human Ovarian Cancer Tissue. MMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human MMP-2 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF902) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry MMP-2 antibody in Human Ovary by Immunohistochemistry (IHC-P). View Larger

MMP‑2 in Human Ovary. MMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian array using Goat Anti-Human MMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF902) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human MMP-2 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human MMP‑2 by Simple WesternTM. Simple Western lane view shows lysates of U-118-MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for MMP-2 at approximately 78 kDa (as indicated) using 10 µg/mL of Goat Anti-Human MMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF902) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-2

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP-2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting; and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites.

Long Name
Matrix Metalloproteinase 2
Entrez Gene IDs
4313 (Human); 17390 (Mouse)
Alternate Names
72 kDa gelatinase; CLG4; CLG4A72 kDa type IV collagenase; collagenase type IV-A; EC 3.4.24; EC 3.4.24.24; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase); matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP2; MMP-2; MMP-II; MONA; neutrophil gelatinase; TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

Product Datasheets

Citations for Human MMP-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
Filter your results:

Filter by:

  1. EDTA/gelatin zymography method to identify C1s versus activated MMP-9 in plasma and immune complexes of patients with systemic lupus erythematosus
    Authors: E Ugarte-Ber, E Martens, L Boon, J Vandooren, D Blockmans, P Proost, G Opdenakker
    J. Cell. Mol. Med., 2019;23(1):576-585.
  2. Glycosaminoglycans influence enzyme activity of MMP2 and MMP2/TIMP3 complex formation - Insights at cellular and molecular level
    Authors: G Ruiz-Gómez, S Vogel, S Möller, MT Pisabarro, U Hempel
    Sci Rep, 2019;9(1):4905.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Age-related lung tissue remodeling due to the local distribution of MMP-2, TIMP-2, TGF-? and Hsp70
    Authors: Z Vitenberga, M Pilmane
    Biotech Histochem, 2018;0(0):1-10.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Pr
  4. Humanin, a cytoprotective peptide, is expressed in carotid atherosclerotic [corrected] plaques in humans.
    Authors: Zacharias DG, Kim SG, Massat AE, Bachar AR, Oh YK, Herrmann J, Rodriguez-Porcel M, Cohen P, Lerman LO, Lerman A
    PLoS ONE, 2012;7(2):e31065.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. ErbB2-enhanced invasiveness of H-Ras MCF10A breast cells requires MMP-13 and uPA upregulation via p38 MAPK signaling.
    Authors: Yong HY, Kim IY, Kim JS, Moon A
    Int. J. Oncol., 2010;36(2):501-7.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  6. Fibroblast-conditioned media promote human sarcoma cell invasion.
    Authors: Bittner JG, Wilson M, Shah MB, Albo D, Feig BW, Wang TN
    Surgery, 2009;145(1):42-7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  8. Claudin-1 overexpression in melanoma is regulated by PKC and contributes to melanoma cell motility.
    Authors: Leotlela PD, Wade MS, Duray PH, Rhode MJ, Brown HF, Rosenthal DT, Dissanayake SK, Earley R, Indig FE, Nickoloff BJ, Taub DD, Kallioniemi OP, Meltzer P, Morin PJ, Weeraratna AT
    Oncogene, 2007;26(26):3846-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Differential expression and activity of matrix metalloproteinases during flow-modulated vein graft remodeling.
    Authors: Berceli SA, Jiang Z, Klingman NV, Pfahnl CL, Abouhamze ZS, Frase CD, Schultz GS, Ozaki CK
    J. Vasc. Surg., 2004;39(5):1084-90.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human MMP-2 Antibody

Average Rating: 4.3 (Based on 4 Reviews)

5 Star
25%
4 Star
75%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human MMP-2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human MMP-2 Antibody
By Anonymous on 07/13/2018
Application: WB Sample Tested: HeLa cells Species: Human

Human MMP-2 Antibody
By Anonymous on 11/09/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

The polyclonal goat antibody AF902 was used as both capture and detection to build and ELISA measuring MMP-2 in human serum and plasma samples.


Human MMP‑2 Antibody
By Anonymous on 02/27/2016
Application: WB Sample Tested: Human cell conditioned medium Species: Human

Human MMP-2 Antibody
By Anonymous on 11/23/2015
Application: WB Sample Tested: Trabecular meshwork endothelial Species: Human, input species here