Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Canine, Fish - Danio rerio (Zebrafish), Rabbit

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry, ELISA Microarray Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MMP-2 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human MMP‑2
Ile34-Cys660
Accession # P08253

Specificity

Detects human MMP-2 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human MMP‑2 Antibody

Detection of Human MMP‑2 antibody by Western Blot.

Detection of Human MMP‑2 by Western Blot.

Western blot shows lysate of U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF902) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for MMP-2 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MMP-2 antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P).

MMP‑2 in Human Ovarian Cancer Tissue.

MMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human MMP-2 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF902) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

MMP-2 antibody in Human Ovary by Immunohistochemistry (IHC-P).

MMP‑2 in Human Ovary.

MMP-2 was detected in immersion fixed paraffin-embedded sections of human ovarian array using Goat Anti-Human MMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF902) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human MMP-2 antibody by Simple WesternTM.

Detection of Human MMP‑2 by Simple WesternTM.

Simple Western lane view shows lysates of U-118-MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. A specific band was detected for MMP-2 at approximately 78 kDa (as indicated) using 10 µg/mL of Goat Anti-Human MMP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF902) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human MMP-2 by Western Blot

Detection of Human MMP-2 by Western Blot

Conditioned media from HCV-expressing cells enhance invasive potential of HSC.(A) Effect of different CM on HSC invasion through transwell inserts precoated with collagen. Same amount of serum starved HSC (5×104 cells/100 µL) were seeded at the upper chambers of transwells and exposed during 24 h to different CM (hepatic-derived CM, 0% FBS DMEM or 10% FBS DMEM) dispensed at the lower compartments. At the end of migration, the upper surface of the membrane was washed and HSC adhered to the lower surface were fixed in methanol, stained with DAPI and counted in 5 randomly chosen microscopic fields (400x) in an epifluorescence microscope (Leica, Wetzlar, Germany). Data from 4 independent experiments are shown as mean +SD. #p<0.05, HSC cultured with CM from HCV replicons or DMEM 10% FBS versus HSC cultured in DMEM 0% FBS; *p<0.05, HSC cultured with CM from HCV-expressing cells or 10% FBS versus HSC cultured with CM from Huh7. (B) ProMMP-2 and MMP-2 expression (AF902, 0.2 µg/mL) and activity were respectively examined by western blotting (WB) and zymography and further quantified in protein extracts (20 µL) of HSC cultured during 24 h with CM from Huh7 or HCV-expressing cells. Bars represent the mean of densitometric analysis from two independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/25302785), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of MMP-2 by Western Blot

Detection of MMP-2 by Western Blot

Conditioned media from G17-stimulated melanoma cells exhibit upregulation of MMP-2 and downregulation of TIMP-3 expression. Representative demonstration of the confirmation of proteomic data using Western blot analysis, with prosaposin serving as a benchmark for gastrin responsiveness (A). Mean and standard deviation (±SD) of the densitometric analysis results from three independent experiments (B). Statistical difference (* p < 0.05) between treated and untreated control groups (considered as 100%) are indicated as follows: prosaposin, TIMP-3 and TIMP-1 levels in G361 cells and MMP2 expressions in the SK-MEL-2 cell line. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1422-0067/24/23/16851), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human MMP‑2 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue and normal human ovarian array

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP‑2 (Catalog # 902-MP), see our available Western blot detection antibodies

Simple Western

10 µg/mL
Sample: U‑118‑MG human glioblastoma/astrocytoma cell line

Western Blot

1 µg/mL
Sample: U‑118‑MG human glioblastoma/astrocytoma cell line

Reviewed Applications

Read 4 reviews rated 4.3 using AF902 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-2

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP-2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting; and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites.

Long Name

Matrix Metalloproteinase 2

Alternate Names

Gelatinase A, MMP2

Entrez Gene IDs

4313 (Human); 17390 (Mouse)

Gene Symbol

MMP2

UniProt

P08253

Additional MMP-2 Products

Product Documents for Human MMP‑2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MMP‑2 Antibody

For research use only

Citations for Human MMP‑2 Antibody

Customer Reviews for Human MMP‑2 Antibody (4)

4.3 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
75%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human MMP-2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HeLa cells
    Species: Human
    Verified Customer | Posted 07/13/2018
  • Human MMP-2 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/09/2017
    The polyclonal goat antibody AF902 was used as both capture and detection to build and ELISA measuring MMP-2 in human serum and plasma samples.
    Human MMP‑2 Antibody AF902
  • Human MMP‑2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human cell conditioned medium
    Species: Human
    Verified Customer | Posted 02/27/2016
    Human MMP‑2 Antibody AF902
  • Human MMP‑2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Trabecular meshwork endothelial
    Species: Human and input species here
    Verified Customer | Posted 11/23/2015

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Protocols

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