Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MMP-8 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MMP‑8
Phe21-Gly467
Accession # AAZ38714

Specificity

Detects human MMP-8 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant mouse MMP-8 and recombinant rat MMP-8 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human MMP‑8 Antibody

MMP-8 antibody in Human Breast by Immunohistochemistry (IHC-P).

MMP‑8 in Human Breast.

MMP-8 was detected in immersion fixed paraffin-embedded sections of human breast array using Goat Anti-Human MMP-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF908) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

MMP-8 antibody in Jurkat Human Cell Line by Immunocytochemistry (ICC).

MMP‑8 in Jurkat Human Cell Line.

MMP-8 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Goat Anti-Human MMP-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF908) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human MMP‑8 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast array

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP‑8 (Catalog # 908-MP), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human MMP‑8 Western Blot Standard (Catalog # WBC017)

Reviewed Applications

Read 1 review rated 3 using AF908 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-8

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-8 (neutrophil collagenase) is expressed in neutrophils, where it is stored in specific granules. MMP-8 release from the neutrophils is stimulated by various factors such as interleukins 1 and 8, TNF-alpha and GM-CSF. MMP-8 is capable of cleaving types I, II and III triple-helical collagen, gelatin peptides, fibronectin, proteoglycans, aggrecan, serpins, beta -casein and peptides such as angiotensin and substance P. In addition to its function in phagocytosis, MMP‑8 has a high capacity for infiltrating connective tissue, and is implicated in the breakdown of the extracellular matrix in diseases such as rheumatoid arthritis. Structurally, MMP‑8 consists of several domains: a pro-domain that is cleaved upon activation, a catalytic domain containing the zinc-binding site, a short hinge region and a hemopexin-like domain. MMP-8 is heavily glycosylated.

Long Name

Matrix Metalloproteinase 8

Alternate Names

Collagenase 2, MMP8

Entrez Gene IDs

4317 (Human); 17394 (Mouse); 63849 (Rat)

Gene Symbol

MMP8

UniProt

AAZ38714

Additional MMP-8 Products

Product Documents for Human MMP‑8 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MMP‑8 Antibody

For research use only

Citations for Human MMP‑8 Antibody

Customer Reviews for Human MMP‑8 Antibody (1)

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  • Human MMP-8 Antibody
    Name: Balaji Mahender
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/15/2018

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Protocols

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