Human MMP-8 Antibody

Catalog # Availability Size / Price Qty
AF908
AF908-SP
MMP‑8 in Human Breast.
2 Images
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)

Human MMP-8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human MMP-8 in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant mouse MMP-8 and recombinant rat MMP-8 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MMP‑8
Phe21-Gly467
Accession # AAZ38714
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human MMP‑8 Western Blot Standard (Catalog # WBC017)
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human MMP‑8 (Catalog # 908-MP), see our available Western blot detection antibodies
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry MMP-8 antibody in Human Breast by Immunohistochemistry (IHC-P). View Larger

MMP‑8 in Human Breast. MMP-8 was detected in immersion fixed paraffin-embedded sections of human breast array using Goat Anti-Human MMP-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF908) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry MMP-8 antibody in Jurkat Human Cell Line by Immunocytochemistry (ICC). View Larger

MMP‑8 in Jurkat Human Cell Line. MMP-8 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Goat Anti-Human MMP-8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF908) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-8

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-8 (neutrophil collagenase) is expressed in neutrophils, where it is stored in specific granules. MMP-8 release from the neutrophils is stimulated by various factors such as interleukins 1 and 8, TNF-alpha and GM-CSF. MMP-8 is capable of cleaving types I, II and III triple-helical collagen, gelatin peptides, fibronectin, proteoglycans, aggrecan, serpins, beta -casein and peptides such as angiotensin and substance P. In addition to its function in phagocytosis, MMP‑8 has a high capacity for infiltrating connective tissue, and is implicated in the breakdown of the extracellular matrix in diseases such as rheumatoid arthritis. Structurally, MMP‑8 consists of several domains: a pro-domain that is cleaved upon activation, a catalytic domain containing the zinc-binding site, a short hinge region and a hemopexin-like domain. MMP-8 is heavily glycosylated.

Long Name
Matrix Metalloproteinase 8
Entrez Gene IDs
4317 (Human); 17394 (Mouse); 63849 (Rat)
Alternate Names
CLG1HNC; Collagenase 2; EC 3.4.24; EC 3.4.24.34; matrix metallopeptidase 8 (neutrophil collagenase); matrix metalloproteinase 8 (neutrophil collagenase); Matrix metalloproteinase-8; MMP8; MMP-8; neutrophil collagenase; PMNL collagenase; PMNL-CL

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Citations for Human MMP-8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Immunoexpression of MMP-8 and MMP-9 in chronic subdural hematoma
    Authors: Gao-Jian Su, Di Zhang, Jia-Nuo Wu, Yu-Hang Deng, Chu-Wei Wu, Xie-Jun Zhang et al.
    Frontiers in Neurology
  2. A Prototype Antibody-based Biosensor for Measurement of Salivary MMP-8 in Periodontitis using Surface Acoustic Wave Technology
    Authors: John J. Taylor, Katrin M. Jaedicke, Rachel C. van de Merwe, Susan M. Bissett, Nichola Landsdowne, Kerry M. Whall et al.
    Scientific Reports
  3. Redundancy of IL-1 Isoform Signaling and Its Implications for Arterial Remodeling.
    Authors: Marina Beltrami-M, Amélie Vromman, Galina K Sukhova, Eduardo J Folco, Peter Libby
    PLoS ONE, 2016-03-31;0(0):1932-6203.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  4. Matrix-metalloproteinase-14 deficiency in bone-marrow-derived cells promotes collagen accumulation in mouse atherosclerotic plaques.
    Authors: Schneider F, Sukhova GK, Aikawa M, Canner J, Gerdes N, Tang SM, Shi GP, Apte SS, Libby P
    Circulation, 2008-02-04;117(7):931-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Reviews for Human MMP-8 Antibody

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Human MMP-8 Antibody
By Balaji Mahender on 03/15/2018
Application: ELISA Sample Tested: EDTA Plasma Species: Human