Human MMR/CD206 Antibody Summary
Leu19-Lys1383 (Thr399Ala) & (Leu407Phe)
Accession # P22897
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human MMR/CD206 by Western Blot. Western blot shows lysates of human immature dendritic cells. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMR/CD206 Monoclonal Antibody (Catalog # MAB25342) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MMR/CD206 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of MMR/CD206 in Human Dendritic Cells by Flow Cytometry Human monocyte-derived immature dendritic cells were stained with Mouse Anti-Human MMR/CD206 Monoclonal Antibody (Catalog # MAB25342, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The human Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 190 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The human MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1371 aa extracellular region, a 21 aa transmembrane segment and a 46 aa cytoplasmic domain (4). Its extracellular region is composed of an N‑terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3, 4). Human to mouse, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N‑acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (5). Several of the CRDs participate in the Ca2+-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (6). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin‑coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor is recycled to the cell surface (3, 7). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).
- East, L. and C. Isake (2002) Biochim. Biophys. Acta 1572:364.
- Chieppa, M. et al. (2003) J. Immunol. 171:4552.
- Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
- Taylor, M. et al. (1990) J. Biol. Chem. 265:12156.
- Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
- Martinez-Pomares, L. et al. (2001) Immunobiology 204:527.
- Feinberg, H. et al. (2000) J. Biol. Chem. 275:21539.
Citations for Human MMR/CD206 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Endothelial extracellular vesicles modulate the macrophage phenotype: Potential implications in atherosclerosis
Authors: S He, C Wu, J Xiao, D Li, Z Sun, M Li
Scand. J. Immunol., 2018;0(0):.
Sample Types: Whole Cells
Applications: Flow Cytometry
O-mannosylation of the Mycobacterium tuberculosis adhesin Apa is crucial for T cell antigenicity during infection but is expendable for protection.
Authors: Nandakumar, Subhadra, Kannanganat, Sunil, Dobos, Karen M, Lucas, Megan, Spencer, John S, Fang, Sunan, McDonald, Melissa, Pohl, Jan, Birkness, Kristin, Chamcha, Venkates, Ramirez, Melissa, Plikaytis, Bonnie B, Posey, James E, Amara, Rama Rao, Sable, Suraj B
PLoS Pathog, 2013;9(10):e1003705.
Sample Types: Whole Cells
Applications: Functional Assay
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