Human/Mouse Acetyl-CoA Carboxylase  alpha /ACACA Antibody

Catalog # Availability Size / Price Qty
MAB6898
MAB6898-SP
Detection of Human and Mouse Acetyl-CoA Carboxylase  alpha /ACACA by Western Blot.
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Human/Mouse Acetyl-CoA Carboxylase  alpha /ACACA Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Acetyl-CoA Carboxylase alpha /ACACA in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 738421
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Acetyl-CoA Carboxylase alpha /ACACA
Pro1185-Phe1352
Accession # Q13085
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human and Mouse Acetyl-CoA Carboxylase  alpha /ACACA by Western Blot. View Larger

Detection of Human and Mouse Acetyl-CoA Carboxylase alpha /ACACA by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, HCT‑116 human colorectal carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Acetyl-CoA Carboxylase alpha /ACACA Monoclonal Antibody (Catalog # MAB6898) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Acetyl-CoA Carboxylase alpha /ACACA at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Acetyl-CoA Carboxylase alpha/ACACA

ACAC-A (Acetyl-CoA carboxylase alpha/1; also ACC-1 and biotin carboxylase) is a 260-265 kDa cytoplasmic, phosphorylated biotinyl-enzyme. It is widely expressed, and found to be concentrated in hepatocytes, adipocytes and lactating mammary epithelium. It is one of two gene products (ACAC-B/beta being the other) that catalyze the formation of malonyl-CoA from acetyl-CoA. The formation of malonyl-CoA by ACAC-A is a rate-limiting step in fatty acid synthesis; malonyl-CoA formed by ACAC-B acts as a regulator of CPT-1 during fatty acidoxidation. Human ACAC-A is 2346 amino acids (aa ) in length. It contains an N-terminal acetylated Met, one ATP-Grasp domain (aa 275-466) with an embedded biotin carboxylation domain (aa 117-618), a biotinyl-binding region (aa 752-818), and a carboxyltransferase domain (aa 1698-2194). There are at least 17 utilized phosphorylation sites, and two acetylated Lys. ACAC-A exists as either a dimer or higher-order oligomer. Multiple splice variants exist. One possesses an alternative start site at Met79, a second utilizes an alternative start site 37 aa upstream of the standard site, and a third (called PIII) shows a 17 aa substitution for aa 1-75. Over aa 1185-1352, human ACAC-A shares 95% aa identity with mouse ACAC-A, and 97% aa identity with both ovine and bovine ACAC-A.

Long Name
Acetyl-Coenzyme A Carboxylase alpha
Entrez Gene IDs
31 (Human); 107476 (Mouse); 60581 (Rat)
Alternate Names
ACACA; ACACacetyl-CoA carboxylase 1; ACC1; ACC1ACC; ACCA; ACC-alpha; AcetylCoA Carboxylase alpha; Acetyl-CoA Carboxylase alpha; acetyl-Coenzyme A carboxylase alpha; EC 6.4.1.2

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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