Detection of Human/Mouse Bax Minus C-Terminus by Western Blot. Western blot shows lysates of L‑929 mouse fibroblast cell line and THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1.0-2.0 µg/mL of Human/Mouse Bax Minus C-Terminus Monoclonal Antibody (Catalog # MAB883) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Bax Minus C-Terminus at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Preparation and Storage
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
Bax belongs to the Bcl2 protein family. It forms homodimers and heterodimers with Bcl2 and functions as an apoptotic activator. Bax interacts with the mitochondrial voltage dependent anion channel and promotes the release of cytochrome c.
Bcl Associated X Protein
Entrez Gene IDs:
581 (Human); 12028 (Mouse); 24887 (Rat)
apoptosis regulator BAX; Bax; BCL2-associated X protein; Bcl2-L-4; BCL2L4bcl2-L-4; Bcl-2-like protein 4
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