Human/Mouse Bcl-w Antibody Summary
Accession # Q92843
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Bcl‑w in Human Spleen. Bcl‑w was detected in immersion fixed paraffin-embedded sections of human spleen using 3 µg/mL Goat Anti-Human/Mouse Bcl‑w Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8241) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Bcl-w is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-w is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-w is required for normal sperm maturation (3, 4, 5). Natural Bcl-w contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-w missing the mitochondrial targeting sequence maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-w appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.
- Gross, A. et al. (1999) Genes and Develop. 13:1899.
- Kroemer, G. (1997) Nature Med. 3:614.
- Ross, J.A., et al. (1998) Nat. Genet. 18:251.
Print, C.G. et al. (1998) Proc. Natl. Acad. Sci. USA 95:12323.
Yan, W. et al. (2000) Mol. Endocrin. 14:682.
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