Human/Mouse BMP-9 Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human BMP-9 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant mouse BMP‑9 is observed. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human (rh) BMP-2, -3, -4, -5, -7, -8, and
    rhBMP‑10 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human BMP-9
    Ser320-Arg429
    Accession # Q9UK05
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human BMP‑9 (Catalog # 3209-BP)
  • Neutralization
    Measured by its ability to neutralize BMP‑9-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND50) is typically 0.08-0.3 µg/mL in the presence of 2 ng/mL Recombinant Human BMP‑9.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Alkaline Phosphatase Production Induced by BMP‑9 and Neutralization by Human BMP‑9 Antibody. Recombinant Human BMP‑9 (Catalog # 3209‑BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phospha­tase production elicited by Recombinant Human BMP‑9 (2 ng/mL) is neutralized (green line) by increasing concen­trations of Goat Anti-Human BMP‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3209). The ND50 is typically 0.08-0.3 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-9

Human BMP-9, also known as growth and differentiation factor 2 (GDF-2), is a member of the BMP subgroup of the TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-9 regulates the development and function of a variety of embryonal and adult tissues (1, 2). The human BMP-9 cDNA encodes a 429 amino acid (aa) precursor that includes a 22 aa signal sequence, a 298 aa propeptide, and a 111 aa mature protein (3). Unlike with other BMP family proteins, the propeptide does not interfere with the biological activity of BMP-9 and remains associated with the mature peptide after proteolytic cleavage (4). Human and mouse BMP-9 share 96% aa sequence identity. Within the mature protein, human BMP-9 shares 64% aa sequence identity with human BMP-10 and less than 50% aa sequence identity with other BMPs. BMP-9 is expressed by non-parenchymal cells in the liver, (5, 6) where it promotes lipid metabolism and inhibits glucose production (7). BMP-9 exerts a prolonged hypoglycemic effect which may be due to an enhancement of insulin release (7). BMP-9 interacts with a high affinity specific heteromeric receptor expressed on liver endothelial cells that has been identified as ALK-1 (4-6). In the embryonal CNS, BMP-9 functions in the development and maintenance of the cholinergic neuronal phenotype (8-10). BMP-9 also induces the differentiation of mesenchymal stem cells into the chondrogenic lineage (11, 12). At low concentrations, BMP-9 is a proliferative factor for hematopoietic progenitor cells, but at higher concentrations, it enhances TGF-beta 1 production and inhibits hematopoietic progenitor colony formation (13).

  • References:
    1. Chen, D. et al. (2004) Growth Factors 22:233.
    2. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
    3. Celeste, A.J. et al. (1994) J. Bone Miner. Res. 9:S136.
    4. Brown, M.A. et al. (2005) J. Biol. Chem. 280:25111.
    5. Song, J.J. et al. (1995) Endocrinology 136:4293.
    6. Miller, A.F. et al. (2000) J. Biol. Chem. 275:17937.
    7. Chen, C. et al. (2003) Nat. Biotechnol. 21:294.
    8. Lopez-Coviella, I. et al. (2000) Science 289:313.
    9. Lopez-Coviella, I. et al. (2005) Proc. Natl. Acad. Sci. USA 102:6984.
    10. Lopez-Coviella, I. et al. (2002) J. Physiol. Paris 96:53.
    11. Majumdar, M.K. et al. (2001) J. Cell. Physiol. 189:275.
    12. Hills, R.L. et al. (2005) J. Orthoped. Res. 23:611.
    13. Ploemacher, R.E. et al. (1999) Leukemia 13:428.
  • Long Name:
    Bone Morphogenetic Protein 9
  • Entrez Gene IDs:
    2658 (Human); 12165 (Mouse)
  • Alternate Names:
    BMP9; BMP-9; BMP9BMP-9Bone morphogenetic protein 9; GDF2; GDF-2; growth differentiation factor 2; growth/differentiation factor 2
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