Human/Mouse/Primate BMP-9 Antibody

  (7 citations)   
Product Details
Citations (7)
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  • Species Reactivity
    Human, Mouse, Primate
  • Specificity
    Detects human and primate BMP‑9 in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human BMP‑1, -1.1, -2, -3, -3b, -4, -5, -6, -7, -8, or -10 is observed. In direct ELISAs, approximately 80% cross-reactivity with recombinant mouse (rm) BMP‑9 and 10% cross‑reactivity with rmBMP-10 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 360107
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human BMP-9
    Ser320-Arg429
    Accession # Q9UK05
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human BMP‑9 (Catalog # 3209-BP) under non-reducing conditions only
    • Human/Primate BMP-9 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Human/Mouse/Primate BMP‑9 Antibody (Catalog # MAB3209)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human/Primate BMP‑9 Biotinylated Antibody (Catalog # BAF3209)
  • ELISA Standard
     
    Recombinant Human BMP-9 Protein (Catalog # 3209-BP)
  • Neutralization
    Measured by its ability to neutralize BMP‑9-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND50) is typically 0.01-0.04 µg/mL in the presence of 5 ng/mL Recombinant Human BMP‑9.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Alkaline Phosphatase Production Induced by BMP‑9 and Neutralization by Human BMP‑9 Antibody.

Recombinant Human BMP‑9 (Catalog # 3209‑BP) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑9 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse/Primate BMP‑9 Monoclonal Antibody (Catalog # MAB3209). The ND50 is typically 0.01-0.04 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-9

Human BMP-9, also known as growth and differentiation factor 2 (GDF-2), is a member of the BMP subgroup of the TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. BMP-9 regulates the development and function of a variety of embryonal and adult tissues (1, 2). The human BMP-9 cDNA encodes a 429 amino acid (aa) precursor that includes a 22 aa signal sequence, a 298 aa propeptide, and a 111 aa mature protein (3). Unlike with other BMP family proteins, the propeptide does not interfere with the biological activity of BMP-9 and remains associated with the mature peptide after proteolytic cleavage (4). Human and mouse BMP-9 share 96% aa sequence identity. Within the mature protein, human BMP-9 shares 64% aa sequence identity with human BMP-10 and less than 50% aa sequence identity with other BMPs. BMP-9 is expressed by non-parenchymal cells in the liver, (5, 6) where it promotes lipid metabolism and inhibits glucose production (7). BMP-9 exerts a prolonged hypoglycemic effect which may be due to an enhancement of insulin release (7). BMP-9 interacts with a high affinity specific heteromeric receptor expressed on liver endothelial cells that has been identified as ALK-1 (4-6). In the embryonal CNS, BMP-9 functions in the development and maintenance of the cholinergic neuronal phenotype (8-10). BMP-9 also induces the differentiation of mesenchymal stem cells into the chondrogenic lineage (11, 12). At low concentrations, BMP-9 is a proliferative factor for hematopoietic progenitor cells, but at higher concentrations, it enhances TGF-beta 1 production and inhibits hematopoietic progenitor colony formation (13).

  • References:
    1. Chen, D. et al. (2004) Growth Factors 22:233.
    2. Miyazono, K. et al. (2005) Cytokine Growth Factor Rev. 16:251.
    3. Celeste, A.J. et al. (1994) J. Bone Miner. Res. 9:S136.
    4. Brown, M.A. et al. (2005) J. Biol. Chem. 280:25111.
    5. Song, J.J. et al. (1995) Endocrinology 136:4293.
    6. Miller, A.F. et al. (2000) J. Biol. Chem. 275:17937.
    7. Chen, C. et al. (2003) Nat. Biotechnol. 21:294.
    8. Lopez-Coviella, I. et al. (2000) Science 289:313.
    9. Lopez-Coviella, I. et al. (2005) Proc. Natl. Acad. Sci. USA 102:6984.
    10. Lopez-Coviella, I. et al. (2002) J. Physiol. Paris 96:53.
    11. Majumdar, M.K. et al. (2001) J. Cell. Physiol. 189:275.
    12. Hills, R.L. et al. (2005) J. Orthoped. Res. 23:611.
    13. Ploemacher, R.E. et al. (1999) Leukemia 13:428.
  • Long Name:
    Bone Morphogenetic Protein 9
  • Entrez Gene IDs:
    2658 (Human); 12165 (Mouse)
  • Alternate Names:
    BMP9; BMP-9; BMP9BMP-9Bone morphogenetic protein 9; GDF2; GDF-2; growth differentiation factor 2; growth/differentiation factor 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Rapid Activation of Bone Morphogenic Protein 9 by Receptor-mediated Displacement of Pro-domains
    Authors: Y Kienast, U Jucknischk, S Scheiblich, M Thier, M de Wouters, A Haas, C Lehmann, V Brand, D Bernicke, K Honold, S Lorenz
    J. Biol. Chem, 2016;291(7):3395-410.
    Species: Human
    Sample Type: Protein
    Application: ELISA - Capture
  2. A mouse model of hereditary hemorrhagic telangiectasia generated by transmammary-delivered immunoblocking of BMP9 and BMP10
    Sci Rep, 2016;5(0):37366.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  3. H3K9 methylation is a barrier during somatic cell reprogramming into iPSCs.
    Authors: Chen, Jiekai, Liu, He, Liu, Jing, Qi, Jing, Wei, Bei, Yang, Jiaqi, Liang, Hanquan, Chen, You, Chen, Jing, Wu, Yaran, Guo, Lin, Zhu, Jieying, Zhao, Xiangjie, Peng, Tianran, Zhang, Yixin, Chen, Shen, Li, Xuejia, Li, Dongwei, Wang, Tao, Pei, Duanqing
    Nat Genet, 2013;45(1):34-42.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  4. Anti-human activin receptor-like kinase 1 (ALK1) antibody attenuates bone morphogenetic protein 9 (BMP9)-induced ALK1 signaling and interferes with endothelial cell sprouting.
    Authors: van Meeteren LA, Thorikay M, Bergqvist S, Pardali E, Stampino CG, Hu-Lowe D, Goumans MJ, ten Dijke P
    J. Biol. Chem., 2012;287(22):18551-61.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. Crossveinless 2 regulates bone morphogenetic protein 9 in human and mouse vascular endothelium.
    Authors: Yao Y, Jumabay M, Ly A, Radparvar M, Wang AH, Abdmaulen R, Bostrom KI
    Blood, 2012;119(21):5037-47.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  6. BMP9 and BMP10 are critical for postnatal retinal vascular remodeling.
    Blood, 2012;119(25):6162-71.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  7. A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
    Authors: Herrera B, Inman GJ,
    BMC Cell Biol., 2009;10(0):20.
    Species: Human
    Sample Type: Recombinant Protein
    Application: Neut

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Mouse IgG2B Isotype Control

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Reconstitution Buffer 1 (PBS)

RB01
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Rat Anti-Mouse IgG2B APC-conjugated Antibody

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IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Mouse IgG2B Antibody

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