Human/Mouse cIAP Pan-specific Antibody

(9 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse cIAP-1 and cIAP-2 in Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 315301
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human cIAP-2
    Asn2-Ser604
    Accession # Q13489
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human/Mouse cIAP by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human cIAP‑1 or human cIAP‑2. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse cIAP Pan-specific Monoclonal Antibody (Catalog # MAB3400) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and SW3T3 mouse contact inhibited fibroblast cell line were included. Specific bands for cIAP-1 and cIAP-2 were detected at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Human cIAP by Simple WesternTM.
Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with either cIAP-1 or cIAP-2 and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for cIAP-1 and cIAP-2 at approximately 73 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse cIAP Pan-specific Monoclonal Antibody (Catalog # MAB3400). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: cIAP (pan)
Cellular inhibitor of apoptosis protein1 (cIAP-1, also known as BIRC2, MIHB, and HIAP2) and 2 (cIAP-2, also known as BIRC3, MIHC, and HIAP1), are members of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. Structurally, cIAP-1 and cIAP-2 are each comprised of 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). Human cIAP-1 and cIAP-2 share 70% sequence identity. Functionally, cIAPs inhibits caspases through the direct interaction of its BIR domain with the active caspase. The ring finger domain of cIAP-1 and -2 also functions as an E3 ubiquitin ligase to ubiqutinylate specific target proteins. Caspase activity may be restored by mitochondrial proteins, such as SMAC/Diablo or HtrA2/Omi, through interactions with the Reaper-like motif and the BIR domain. cIAPs are reported to be cleaved by HtrA2/Omi.
  • Long Name:
    Cellular Inhibitor of Apoptosis Protein
  • Alternate Names:
    BIRC; cIAP; HIAP
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
Applications
Sample Type
  1. Characterization of Potent SMAC Mimetics that Sensitize Cancer Cells to TNF Family-Induced Apoptosis
    PLoS ONE, 2016;11(9):e0161952.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Synergistic Activity of Deguelin and Fludarabine in Cells from Chronic Lymphocytic Leukemia Patients and in the New Zealand Black Murine Model
    Authors: N Rebolleda, I Losada-Fer, G Perez-Chac, R Castejon, S Rosado, M Morado, MT Vallejo-Cr, A Martinez, JA Vargas-Nuñ, P Perez-Acie
    PLoS ONE, 2016;11(4):e0154159.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. IGF2BP1 controls cell death and drug resistance in rhabdomyosarcomas by regulating translation of cIAP1.
    Authors: Faye M, Beug S, Graber T, Earl N, Xiang X, Wild B, Langlois S, Michaud J, Cowan K, Korneluk R, Holcik M
    Oncogene, 2015;34(12):1532-41.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. A RIPK3-caspase 8 complex mediates atypical pro-IL-1beta processing.
    Authors: Moriwaki K, Bertin J, Gough P, Chan F
    J Immunol, 2015;194(4):1938-44.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  5. TRAIL activates JNK and NF-kappaB through RIP1-dependent and -independent pathways.
    Authors: Zhang L, Dittmer M, Blackwell K, Workman L, Hostager B, Habelhah H
    Cell Signal, 2015;27(2):306-14.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Kinase-independent feedback of the TAK1/TAB1 complex on BCL10 turnover and NF-kappaB activation.
    Authors: Moreno-Garcia M, Sommer K, Rincon-Arano H, Brault M, Ninomiya-Tsuji J, Matesic L, Rawlings D
    Mol Cell Biol, 2013;33(6):1149-63.
    Species: Chicken
    Sample Type: Cell Lysates
    Application: WB
  7. TNFR-Associated Factor 2 Deficiency in B Lymphocytes Predisposes to Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma in Mice.
    Authors: Perez-Chacon G, Llobet D, Pardo C, Pindado J, Choi Y, Reed JC, Zapata JM
    J. Immunol., 2012;189(2):1053-61.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  8. Linear ubiquitination prevents inflammation and regulates immune signalling.
    Authors: Gerlach B, Cordier SM, Schmukle AC, Emmerich CH, Rieser E, Haas TL, Webb AI, Rickard JA, Anderton H, Wong WW, Nachbur U, Gangoda L, Warnken U, Purcell AW, Silke J, Walczak H
    Nature, 2011;471(7340):591-6.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  9. Zinc-finger protein 91 plays a key role in LIGHT-induced activation of non-canonical NF-kappaB pathway.
    Authors: Jin HR, Jin X, Lee JJ
    Biochem. Biophys. Res. Commun., 2010;400(4):581-6.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A Isotype Control

Ctrl MAB0031 7  
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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse IgG2A Antibody

Flow MAB0032  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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