Human/Mouse cIAP Pan-specific Antibody

Catalog # Availability Size / Price Qty
MAB3400
MAB3400-SP
Detection of Human/Mouse cIAP by Western Blot.
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Product Details
Citations (13)
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Human/Mouse cIAP Pan-specific Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse cIAP-1 and cIAP-2 in Western blots.
Source
Monoclonal Mouse IgG2A Clone # 315301
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human cIAP-2
Asn2-Ser604
Accession # Q13489
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
10 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human/Mouse cIAP antibody by Western Blot. View Larger

Detection of Human/Mouse cIAP by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human cIAP-1 or human cIAP-2. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse cIAP Pan-specific Monoclonal Antibody (Catalog # MAB3400) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, lysates of Raji human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and SW3T3 mouse contact inhibited fibroblast cell line were included. Specific bands for cIAP-1 and cIAP-2 were detected at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western Detection of Human cIAP antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human cIAP by Simple WesternTM. Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line transfected with either cIAP-1 or cIAP-2 and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for cIAP-1 and cIAP-2 at approximately 73 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse cIAP Pan-specific Monoclonal Antibody (Catalog # MAB3400). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: cIAP (pan)

Cellular inhibitor of apoptosis protein1 (cIAP-1, also known as BIRC2, MIHB, and HIAP2) and 2 (cIAP-2, also known as BIRC3, MIHC, and HIAP1), are members of the inhibitor of apoptosis (IAP) family of proteins that inhibit the proteolytic activity of mature caspases. Structurally, cIAP-1 and cIAP-2 are each comprised of 3 BIR (baculovirus inhibitor of apoptosis) domains, a RING finger domain, and a caspase recruitment domain (CARD). Human cIAP-1 and cIAP-2 share 70% sequence identity. Functionally, cIAPs inhibits caspases through the direct interaction of its BIR domain with the active caspase. The ring finger domain of cIAP-1 and -2 also functions as an E3 ubiquitin ligase to ubiqutinylate specific target proteins. Caspase activity may be restored by mitochondrial proteins, such as SMAC/Diablo or HtrA2/Omi, through interactions with the Reaper-like motif and the BIR domain. cIAPs are reported to be cleaved by HtrA2/Omi.

Long Name
Cellular Inhibitor of Apoptosis Protein
Alternate Names
BIRC; cIAP; HIAP

Product Datasheets

Citations for Human/Mouse cIAP Pan-specific Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Bcl-3 promotes TNF-induced hepatocyte apoptosis by regulating the deubiquitination of RIP1
    Authors: Y Hu, H Zhang, N Xie, D Liu, Y Jiang, Z Liu, D Ye, S Liu, X Chen, C Li, Q Wang, X Huang, Y Liu, Y Shi, X Zhang
    Cell Death and Differentiation, 2021;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Opposing roles of hematopoietic-specific small GTPase Rac2 and the guanine nucleotide exchange factor Vav1 in osteoclast differentiation
    Authors: IS Kang, JS Jang, C Kim
    Sci Rep, 2020;10(1):7024.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. The IAP Antagonist SM-164 Eliminates Triple-Negative Breast Cancer Metastasis to Bone and Lung in Mice
    Authors: W Lei, R Duan, J Li, X Liu, A Huston, BF Boyce, Z Yao
    Sci Rep, 2020;10(1):7004.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Disruption of XIAP-RIP2 Association Blocks NOD2-Mediated Inflammatory Signaling
    Authors: T Goncharov, S Hedayati, MM Mulvihill, A Izrael-Tom, K Zobel, S Jeet, AV Fedorova, C Eidenschen, J deVoss, K Yu, AS Shaw, DS Kirkpatric, WJ Fairbrothe, K Deshayes, D Vucic
    Mol. Cell, 2018;69(4):551-565.e7.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Synergistic Activity of Deguelin and Fludarabine in Cells from Chronic Lymphocytic Leukemia Patients and in the New Zealand Black Murine Model
    Authors: N Rebolleda, I Losada-Fer, G Perez-Chac, R Castejon, S Rosado, M Morado, MT Vallejo-Cr, A Martinez, JA Vargas-Nuñ, P Perez-Acie
    PLoS ONE, 2016;11(4):e0154159.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Characterization of Potent SMAC Mimetics that Sensitize Cancer Cells to TNF Family-Induced Apoptosis
    PLoS ONE, 2016;11(9):e0161952.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. TRAIL activates JNK and NF-kappaB through RIP1-dependent and -independent pathways.
    Authors: Zhang L, Dittmer M, Blackwell K, Workman L, Hostager B, Habelhah H
    Cell Signal, 2015;27(2):306-14.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. A RIPK3-caspase 8 complex mediates atypical pro-IL-1beta processing.
    Authors: Moriwaki K, Bertin J, Gough P, Chan F
    J Immunol, 2015;194(4):1938-44.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. IGF2BP1 controls cell death and drug resistance in rhabdomyosarcomas by regulating translation of cIAP1.
    Authors: Faye M, Beug S, Graber T, Earl N, Xiang X, Wild B, Langlois S, Michaud J, Cowan K, Korneluk R, Holcik M
    Oncogene, 2015;34(12):1532-41.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Kinase-independent feedback of the TAK1/TAB1 complex on BCL10 turnover and NF-kappaB activation.
    Authors: Moreno-Garcia M, Sommer K, Rincon-Arano H, Brault M, Ninomiya-Tsuji J, Matesic L, Rawlings D
    Mol Cell Biol, 2013;33(6):1149-63.
    Species: Chicken
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. TNFR-Associated Factor 2 Deficiency in B Lymphocytes Predisposes to Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma in Mice.
    Authors: Perez-Chacon G, Llobet D, Pardo C, Pindado J, Choi Y, Reed JC, Zapata JM
    J. Immunol., 2012;189(2):1053-61.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Linear ubiquitination prevents inflammation and regulates immune signalling.
    Authors: Gerlach B, Cordier SM, Schmukle AC, Emmerich CH, Rieser E, Haas TL, Webb AI, Rickard JA, Anderton H, Wong WW, Nachbur U, Gangoda L, Warnken U, Purcell AW, Silke J, Walczak H
    Nature, 2011;471(7340):591-6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Zinc-finger protein 91 plays a key role in LIGHT-induced activation of non-canonical NF-kappaB pathway.
    Authors: Jin HR, Jin X, Lee JJ
    Biochem. Biophys. Res. Commun., 2010;400(4):581-6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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