Human/Mouse Crk Antibody

Catalog # Availability Size / Price Qty
Detection of Human and Mouse Crk by Western Blot.
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Product Details
Supplemental Products

Human/Mouse Crk Antibody Summary

Species Reactivity
Human, Mouse
Detects human Crk in direct ELISAs and human and mouse Crk in Western blots.
Monoclonal Mouse IgG2B Clone # 463414
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Crk
Accession # P46108
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse Crk antibody by Western Blot. View Larger

Detection of Human and Mouse Crk by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Crk Monoclonal Antibody (Catalog # MAB4316) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Crk at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Crk

Crk belongs to a family of adaptor molecules that are widely expressed in numerous tissues. It was originally isolated from a chicken tumor sample, and despite lacking enzymatic activity, this protein is involved in many signal transduction pathways leading to phosphorylation of multiple proteins. Crk forms multi protein complexes via it’s SH2 (aa 13-118) and SH3 (aa 132-192 and aa 237-296) domains leading to activation of downstream molecules involved in growth, differentiation, cell adhesion and migration. Alternate splicing generates an additional isoform that lacks aa 205-304.

Long Name
V-Crk Sarcoma Virus CT10 Oncogene Homolog
Entrez Gene IDs
1398 (Human); 12928 (Mouse); 54245 (Rat)
Alternate Names
adapter molecule crk; avian sarcoma virus CT10 (v-crk) oncogene homolog; Crk; CRKII; p38; Proto-oncogene c-Crk; v-crk avian sarcoma virus CT10 oncogene homolog; v-crk sarcoma virus CT10 oncogene homolog (avian)

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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