Human/Mouse Cyclin A2 Antibody

Catalog # Availability Size / Price Qty
AF5999
AF5999-SP
Detection of Human and Mouse Cyclin A2 by Western Blot.
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Product Details
Citations (4)
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Human/Mouse Cyclin A2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Cyclin A2 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Cyclin A2
Ala73-Tyr199
Accession # P20248
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human and Mouse Cyclin A2 antibody by Western Blot. View Larger

Detection of Human and Mouse Cyclin A2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Cyclin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5999) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cyclin A2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cyclin A2

Cyclin A2 (also CCNA2) is a member of the cyclin AB subfamily, cyclin family of proteins. Cyclin A2 is ubiquitously expressed, in contrast to CyclinA1 that only appears in late pachytene spermatocytes. It associates with CDK1 during G2 and M phase, and CDK2 in S phase, of the cell cycle. Binding to SCAPER restricts Cyclin A2 to the cytoplasm. In both compartments, it provides substrate specificity to a phosphorylating complex that promotes entry into, and progression through, mitosis. Human Cyclin A2 is 432 amino acids (aa) in length. It contains two cyclin box folds (aa 210‑299 and 309‑430) and four acetylation sites that induce molecule turnover (Lys54/68/95/112). Over aa 73‑199, human Cyclin A2 shares 83% aa identity with mouse Cyclin A2.

Entrez Gene IDs
890 (Human); 12428 (Mouse); 114494 (Rat)
Alternate Names
CCN1CCNAcyclin-A; CCNA; CCNA2; Cyclin A2; Cyclin-A; cyclin-A2

Product Datasheets

Citations for Human/Mouse Cyclin A2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Cisplatin exposure causes c-Myc-dependent resistance to CDK4/6 inhibition in HPV-negative head and neck squamous cell carcinoma
    Authors: AM Robinson, R Rathore, NJ Redlich, DR Adkins, T VanArsdale, BA Van Tine, LS Michel
    Cell Death Dis, 2019;10(11):867.
    Species: Xenograft
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Chemotherapy-induced differential cell cycle arrest in B cell lymphomas affects their sensitivity to Wee1 inhibition
    Authors: X Wang, Z Chen, AK Mishra, A Silva, W Ren, Z Pan, JH Wang
    Haematologica, 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Chemerin, a novel peroxisome proliferator-activated receptor gamma (PPARgamma) target gene that promotes mesenchymal stem cell adipogenesis.
    Authors: Muruganandan S, Parlee SD, Rourke JL, Ernst MC, Goralski KB, Sinal CJ
    J. Biol. Chem., 2011;286(27):23982-95.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. CREB is activated by muscle injury and promotes muscle regeneration.
    Authors: Stewart R, Flechner L, Montminy M
    PLoS ONE, 2011;6(9):e24714.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Secondary Antibodies

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