Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Xenograft

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Cyclin A2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cyclin A2
Ala73-Tyr199
Accession # P20248

Specificity

Detects human and mouse Cyclin A2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse Cyclin A2 Antibody

Detection of Human and Mouse Cyclin A2 antibody by Western Blot.

Detection of Human and Mouse Cyclin A2 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse Cyclin A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5999) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cyclin A2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse Cyclin A2 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and NIH‑3T3 mouse embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cyclin A2

Cyclin A2 (also CCNA2) is a member of the cyclin AB subfamily, cyclin family of proteins. Cyclin A2 is ubiquitously expressed, in contrast to CyclinA1 that only appears in late pachytene spermatocytes. It associates with CDK1 during G2 and M phase, and CDK2 in S phase, of the cell cycle. Binding to SCAPER restricts Cyclin A2 to the cytoplasm. In both compartments, it provides substrate specificity to a phosphorylating complex that promotes entry into, and progression through, mitosis. Human Cyclin A2 is 432 amino acids (aa) in length. It contains two cyclin box folds (aa 210‑299 and 309‑430) and four acetylation sites that induce molecule turnover (Lys54/68/95/112). Over aa 73‑199, human Cyclin A2 shares 83% aa identity with mouse Cyclin A2.

Alternate Names

CCNA, CCNA2

Entrez Gene IDs

890 (Human); 12428 (Mouse); 114494 (Rat)

Gene Symbol

CCNA2

UniProt

P20248 (Human)

Additional Cyclin A2 Products

Product Documents for Human/Mouse Cyclin A2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Cyclin A2 Antibody

For research use only

Citations for Human/Mouse Cyclin A2 Antibody

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