Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 632813
View all DDX5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DDX5
Asn448-Gln614
Accession # P17844

Specificity

Detects human and mouse DDX5 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse DDX5 Antibody

Detection of Human and Mouse DDX5 antibody by Western Blot.

Detection of Human and Mouse DDX5 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse DDX5 Monoclonal Antibody (Catalog # MAB6370) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DDX5 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse DDX5 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, and NIH‑3T3 mouse embryonic fibroblast cell line

Reviewed Applications

Read 1 review rated 5 using MAB6370 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DDX5

DDX5, also known as p68 RNA helicase, is a 614 amino acid (aa), 68 kDa member of the DEAD box family of RNA helicases that contain the conserved Asp‑Glu‑Ala‑Asp (DEAD) motif. DDX5 influences RNA transport, transcription, ribosome assembly, spliceosome function, RNA degradation, and the initiation of translation. DDX5 shares 87% aa identity with DDX17 within the central region and the two are often expressed together. The C-terminal region used as an immunogen shares 96% and 99% aa identity with mouse and rat DDX5, respectively, but lies mainly outside the DDX17 homology region.

Long Name

DEAD Box Protein 5

Alternate Names

DEAD/H, G17P1, HELR, HLR1, HUMP68

Entrez Gene IDs

1655 (Human); 13207 (Mouse); 287765 (Rat)

Gene Symbol

DDX5

UniProt

P17844 (Human)

Additional DDX5 Products

Product Documents for Human/Mouse DDX5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse DDX5 Antibody

For research use only

Citations for Human/Mouse DDX5 Antibody

Customer Reviews for Human/Mouse DDX5 Antibody (1)

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  • Human/Mouse DDX5 Antibody
    Name: Masashi Okada
    Application: Western Blot
    Sample Tested: glioblastoma cell lysates
    Species: Human
    Verified Customer | Posted 11/04/2023
    10 ug each, control, drug treated whole cell lysate marker: 100, 75, 50 kDa 1/3000 diluted pri Ab
    Human/Mouse DDX5 Antibody MAB6370

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