ELF3 Antibody (662533)

R&D Systems | Catalog # MAB5787

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 662533
View all ELF3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ELF3
Met1-Gly173
Accession # P78545

Specificity

Detects human and mouse ELF3 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for ELF3 Antibody (662533)

Detection of Human and Mouse ELF3 antibody by Western Blot.

Detection of Human and Mouse ELF3 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, PC-3 human prostate cancer cell line, HT-29 human colon adenocarcinoma cell line, EL-4 mouse lymphoblast cell line, and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse ELF3 Monoclonal Antibody (Catalog # MAB5787) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ELF3 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for ELF3 Antibody (662533)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, PC‑3 human prostate cancer cell line, HT‑29 human colon adenocarcinoma cell line, EL‑4 mouse lymphoblast cell line, and A549 human lung carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ELF3

ELF3 (E74-Like Factor 3), also known as ESE-1, ESX, ERT and JEN, is a 41-43 kDa member of the ETS family of proteins.  During uncomplicated (non-inflammatory) periods of cell differentiation, ELF3 is expressed exclusively by epithelial cells, repressing genes needed during early differentiation, and promoting genes needed for full differentiation.  Under conditions of inflammation, cells such as monocytes, endothelial cells and chrondrocytes express ELF3 and produce molecules such as Ang1 and COX2.  Human ELF3 is 371 amino acids (aa) in length.  It contains one PNT/pointed dimerization domain (aa 46-132), a protein stabilizing PEST sequence (aa 210-225), an A/T Hook region that binds to AT-rich DNA sequences (aa 236-252), and an ETS DNA binding domain (aa 273-355).  ELF3 interacts with CREBBP, EP300, KU70 and KU86.  There is one splice variant that shows a deletion of aa 174-200.  Over aa 1-173, human ELF3 shares 87% aa identity with mouse ELF3.

 

Long Name

E74-like factor 3

Alternate Names

EPR1, ERT, ESX, JEN

Entrez Gene IDs

1999 (Human); 13710 (Mouse); 304815 (Rat)

Gene Symbol

ELF3

UniProt

P78545

Additional ELF3 Products

Product Documents for ELF3 Antibody (662533)

Certificate of Analysis

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Product Specific Notices for ELF3 Antibody (662533)

For research use only

Citations for ELF3 Antibody (662533)

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