Human/Mouse ELF3 Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse ELF3 in Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 662533
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human ELF3
    Met1-Gly173
    Accession # P78545
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse ELF3 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, PC‑3 human prostate cancer cell line, HT‑29 human colon adenocarcinoma cell line, EL‑4 mouse lymphoblast cell line, and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse ELF3 Monoclonal Antibody (Catalog # MAB5787) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ELF3 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ELF3

ELF3 (E74-Like Factor 3), also known as ESE-1, ESX, ERT and JEN, is a 41-43 kDa member of the ETS family of proteins.  During uncomplicated (non-inflammatory) periods of cell differentiation, ELF3 is expressed exclusively by epithelial cells, repressing genes needed during early differentiation, and promoting genes needed for full differentiation.  Under conditions of inflammation, cells such as monocytes, endothelial cells and chrondrocytes express ELF3 and produce molecules such as Ang1 and COX2.  Human ELF3 is 371 amino acids (aa) in length.  It contains one PNT/pointed dimerization domain (aa 46-132), a protein stabilizing PEST sequence (aa 210-225), an A/T Hook region that binds to AT-rich DNA sequences (aa 236-252), and an ETS DNA binding domain (aa 273-355).  ELF3 interacts with CREBBP, EP300, KU70 and KU86.  There is one splice variant that shows a deletion of aa 174-200.  Over aa 1-173, human ELF3 shares 87% aa identity with mouse ELF3.

 

  • Long Name:
    E74-like factor 3
  • Entrez Gene IDs:
    1999 (Human); 13710 (Mouse); 304815 (Rat)
  • Alternate Names:
    E74-like factor 3 (ets domain transcription factor, epithelial-specific ); E74-like factor 3; ELF3; Epithelial-restricted with serine box; epithelial-specific); Epithelium-restricted Ets protein ESX; Epithelium-specific Ets transcription factor 1; EPR1; EPR-1; ERT; ESX; ets domain transcription factor, serine box (epithelial-specific); ETS-related transcription factor Elf-3; JEN; serine box
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Mouse IgG2B Antibody

Flow MAB0043  
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