Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ERK5/BMK1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ERK5/BMK1
Met1-Glu432
Accession # Q13164

Specificity

Detects human and mouse ERK5/BMK1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse ERK5/BMK1 Antibody

Detection of Human/Mouse ERK5/BMK1 antibody by Western Blot.

Detection of Human/Mouse ERK5/BMK1 by Western Blot.

Western blot shows lysates of MDA-MB-468 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse ERK5/BMK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2848) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ERK5/BMK1 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
ERK5/BMK1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

ERK5/BMK1 in HeLa Human Cell Line.

ERK5/BMK1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse ERK5/BMK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2848) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human/Mouse ERK5/BMK1 Antibody

Application
Recommended Usage

Immunocytochemistry

10-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells and HeLa human cervical epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: MDA-MB-468 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, and Neuro-2A mouse neuroblastoma cell line

Reviewed Applications

Read 1 review rated 5 using AF2848 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ERK5/BMK1

Extracellular signal-regulated kinase 5 (ERK5), also known as big mitogen-activated protein kinase 1 (BMK1) and MAPK7, is activated by a several mechanisms, including receptor tyrosine kinases, G protein-coupled receptors, and osmotic stress. Like ERK1 and ERK2, ERK5 contains the conserved Thr-Glu-Tyr activation motif in its activation loop. Unlike these ERKs, however, ERK5 contains a unique C-terminal domain that regulates its activation and nuclear translocation. ERK5’s upstream MAPK kinase has been identified as MEK5.

Long Name

Extracellular Signal-regulated Kinase 5

Alternate Names

BMK1, MAPK7

Entrez Gene IDs

5598 (Human); 23939 (Mouse)

Gene Symbol

MAPK7

UniProt

Q13164

Additional ERK5/BMK1 Products

Product Documents for Human/Mouse ERK5/BMK1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse ERK5/BMK1 Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Intracellular Kinases

Citations for Human/Mouse ERK5/BMK1 Antibody

Customer Reviews for Human/Mouse ERK5/BMK1 Antibody (1)

5 out of 5
1 Customer Rating
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1 Stars
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  • Human/Mouse ERK5/BMK1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 07/21/2018
    Human/Mouse ERK5/BMK1 Antibody AF2848

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Protocols

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