Human/Mouse ERK5/BMK1 Antibody

Catalog # Availability Size / Price Qty
AF2848
AF2848-SP
Detection of Human/Mouse ERK5/BMK1 by Western Blot.
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse ERK5/BMK1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse ERK5/BMK1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human ERK5/BMK1
Met1-Glu432
Accession # Q13164
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
10-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse ERK5/BMK1 antibody by Western Blot. View Larger

Detection of Human/Mouse ERK5/BMK1 by Western Blot. Western blot shows lysates of MDA-MB-468 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse ERK5/BMK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2848) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ERK5/BMK1 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry ERK5/BMK1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

ERK5/BMK1 in HeLa Human Cell Line. ERK5/BMK1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse ERK5/BMK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2848) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ERK5/BMK1

Extracellular signal-regulated kinase 5 (ERK5), also known as big mitogen-activated protein kinase 1 (BMK1) and MAPK7, is activated by a several mechanisms, including receptor tyrosine kinases, G protein-coupled receptors, and osmotic stress. Like ERK1 and ERK2, ERK5 contains the conserved Thr-Glu-Tyr activation motif in its activation loop. Unlike these ERKs, however, ERK5 contains a unique C-terminal domain that regulates its activation and nuclear translocation. ERK5’s upstream MAPK kinase has been identified as MEK5.

Long Name
Extracellular Signal-regulated Kinase 5
Entrez Gene IDs
5598 (Human); 23939 (Mouse)
Alternate Names
Big MAP kinase 1; BMK1 kinase; BMK1; BMK-1; BMK1EC 2.7.11.24; EC 2.7.11; ERK5; ERK-5; ERK5MAPK 7; Extracellular signal-regulated kinase 5; extracellular-signal-regulated kinase 5; MAP kinase 7; MAPK7; mitogen-activated protein kinase 7; PRKM7ERK4

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Citations for Human/Mouse ERK5/BMK1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Memantine protects against ischemia/reperfusion-induced brain endothelial permeability
    Authors: Y Liu, Y Huang, Y Xu, P Qu, M Wang
    IUBMB Life, 2018-03-15;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Statin regulated ERK5 stimulates tight junction formation and reduces permeability in human cardiac endothelial cells
    Authors: Emma L. Wilkinson, James E. Sidaway, Michael J. Cross
    Journal of Cellular Physiology
  3. Erk5 is a mediator to TGFbeta1-induced loss of phenotype and function in human podocytes.
    Authors: Badshah I, Baines D, Dockrell M
    Front Pharmacol, 2014-04-21;5(0):71.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. TGF-beta activates ERK5 in human renal epithelial cells.
    Authors: Browne JA, Pearson AL, Zahr RA, Niculescu-Duvaz I, Baines DL, Dockrell ME
    Biochem. Biophys. Res. Commun., 2008-06-25;373(3):440-4.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human/Mouse ERK5/BMK1 Antibody

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Human/Mouse ERK5/BMK1 Antibody
By Anonymous on 07/21/2018
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Human