Human/Mouse GDF‑3 Antibody

GDF‑3 in Mouse Thymus.

GDF-3 was detected in perfusion fixed frozen sections of mouse thymus using 5 µg/mL Mouse GDF-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF958) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of GDF-3 by Immunohistochemistry

Brd4-CKO mice had reduced expression of Gdf3 in ATMs.(A) PCA of RNA-Seq data derived from CD11b+ ATMs of WT and Brd4-CKO mice fed a HFD for 20 weeks. (B) Volcano plot of mRNA-Seq analysis in ATMs as indicated in A. Brown dots represent genes increased in ATMs of Brd4-CKO mice vs. WT mice (fold change ≥ 2, adjusted P value ≤ 0.001, calculated by raw count value). Blue dots represent genes decreased in ATMs of Brd4-CKO mice vs. WT mice (fold change ≥ 2, adjusted P value ≤ 0.001, calculated by raw count value). Gray dots represent genes without significantly altered expression. Clusters of significantly altered genes (fold change ≥ 2, adjusted P value ≤ 0.001, calculated by raw count value) were identified using gene ontology terms (C) and KEGG pathways (D). (E) Heat map of the relative expression levels (scaled Z-score) of cytokine-cytokine receptor interaction-related genes clustered in (D). (F) mRNA levels of Gdf3 in CD11b+ ATMs isolated from WT or Brd4-CKO mice fed a HFD for 20 weeks. (G) Left panel: Gdf3 or F4/80 IHC staining of eWAT of WT or Brd4-CKO mice fed a HFD for 20 weeks. Right panel: statistical analysis of Gdf3-positive or F4/80-positive area percentage, the ratio of Gdf3-positive cells in F4/80-positive cells in eWAT of WT and Brd4-CKO mice fed a HFD. Data are mean and SD and are determined by an unpaired 2-tailed Student’s t test. n = 3 mice. **P < 0.01. Brd4-CKO, myeloid lineage-specific Brd4 knockout; HFD, high-fat diet–induced; eWAT, epididymal WAT; ATMs, adipose tissue macrophages; PCA, principal component analysis. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33830083), licensed under a CC-BY license. Not internally tested by R&D Systems.