Glucokinase/GCK Antibody (849520)

R&D Systems | Catalog # MAB7840

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 849520
View all Glucokinase/GCK Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Glucokinase/GCK
Val16-Gln465
Accession # P35557

Specificity

Detects human Glucokinase/GCK in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human PRF-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Glucokinase/GCK Antibody (849520)

Detection of Human and Mouse Glucokinase/GCK antibody by Western Blot.

Detection of Human and Mouse Glucokinase/GCK by Western Blot.

Western blot shows lysates of Huh-7 human hepatoma cell line, Hepa 1-6 mouse hepatoma cell line, and HT-2 mouse T cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Glucokinase/GCK Monoclonal Antibody (Catalog # MAB7840) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glucokinase/GCK at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Glucokinase/GCK Antibody (849520)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Huh‑7 human hepatoma cell line, Hepa 1‑6 mouse hepatoma cell line, and HT‑2 mouse T cell line

Reviewed Applications

Read 1 review rated 4 using MAB7840 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glucokinase/GCK

Hexokinases phosphorylate hexose to form hexose 6‑phosphate, the first step for hexose metabolism. There are four mammalian hexokinases (I, II, III and IV) and hexokinase IV is commonly known as glucose kinase (GCK). Unlike hexokinase I, II and III, which have high affinity for glucose and are strongly inhibited by the product, glucose‑6‑phosphate (1), GCK has much lower affinity for glucose and is not inhibited by the product (2, 3). Consequently, GCK has a Km for glucose of approximately 7 mM (4), which is 100 times higher than that of hexokinase I, II, and III. This unique enzymatic property of GCK allows it to respond to blood glucose levels and contribute to the maintenance of blood glucose levels within the normal physiological range of 4 mM to 6 mM. In the pancreatic islets, GCK serves as a glucose sensor to control insulin release in the beta cells, and to control glucagon release in the alpha cells (5). In hepatocytes, GCK responds to changes of ambient glucose levels by increasing or reducing glycogen synthesis. Mutations in GCK have been associated with non‑insulin‑dependent diabetes mellitus (6, 7), maturity‑onset diabetes of the young type 2 (8), and hyperinsulinemia of infancy (9). The enzyme activity was measured using a phosphatase coupled kinase assay (4).

References

  1. Aleshin, A.E. et al.(1998) Structure 6:39.
  2. Takeda, J. et al. (1993) J. Biol. Chem. 268:15200.
  3. Lange, A.J. et al. (1991) Biochem. J. 277:159.
  4. Wu, Z.L. (2011) PLoS ONE 6(8):e23172.
  5. Xu, L.Z. et al. (1995) J. Biol. Chem. 270:9939.
  6. Gidh-Jain, M. et al. (1993) Proc. Natl. Acad. Sci. U. S. A. 90:1932.
  7. Stoffel, M. et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89:7698.
  8. Hattersley, A.T. et al. (1992) Lancet 339:1307.
  9. Gloyn, A.L. et al. (2003) Diabetes 52:2433.

Long Name

Glucose Kinase

Alternate Names

FGQTL3, GCK, GLK, Hexokinase-4, Hexokinase-D, HHF3, HK4, HXKP, LGLK

Entrez Gene IDs

2645 (Human); 103988 (Mouse); 24385 (Rat)

Gene Symbol

GCK

UniProt

P35557 (Human)

Additional Glucokinase/GCK Products

Product Documents for Glucokinase/GCK Antibody (849520)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Glucokinase/GCK Antibody (849520)

For research use only

Citations for Glucokinase/GCK Antibody (849520)

Customer Reviews for Glucokinase/GCK Antibody (849520) (1)

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  • Human/Mouse Glucokinase/GCK Antibody
    Name: Hua Jiang
    Application: Western Blot
    Sample Tested: Liver tissue, Pancreas tissue, Kidney tissue and Adult brain (hippocampus)
    Species: Human and Mouse
    Verified Customer | Posted 11/18/2016
    Glucokinase/GCK Antibody (849520) MAB7840

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