Detects human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 in direct ELISAs, and human and mouse HS3ST4 in Western blots. In Western blots, no cross-reactivity with recombinant human HS3ST3B1 is observed.
Monoclonal Mouse IgG2A Clone # 712011
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Gly184-Lys456 Accession # Q9Y661
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Mouse Heparan Sulfate 3‑O‑Sulfotransferase 4/HS3ST4 by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line, Neuro‑2A mouse neuroblastoma cell line, U‑118‑MG human glioblastoma/astrocytoma cell line, and human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Heparan Sulfate 3‑O‑Sulfotransferase 4/HS3ST4 Monoclonal Antibody (Catalog # MAB60851) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Heparan Sulfate 3‑O‑Sulfotransferase 4/HS3ST4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Heparan sulfate is a highly sulfated polysaccharide that can be found on
the cell surface and within the extracellular matrix. It is typically
covalently attached to the protein core of proteoglycans, such as
syndecans and glypicans. Heparin, on the other hand, can be considered
as a highly sulfated version of heparan sulfate that is predominantly
found in mast cells. Both heparin and heparan sulfate contain
disaccharide repeats of uronic acid and N‑acetylglucosamine and are
modified by the same sulfotransferases (1, 2). The uronic acid residues
can be sulfated at the 2-O position by heparan sulfate 2-O
sulfotransferase (HS2ST). The N‑acetylglucosamine residues can be
sulfated at the N, 3-O, and 6-O positions by
N‑deacetylase/N‑sulfotransferases (NDSTs), heparan sulfate 3-O
sulfotransferases (HS3STs) and heparan sulfate 6-O sulfotransferases
(HS6STs) respectively. There are seven HS3STs in the human genome (3,
4). HS3ST4 and HS3ST2 are brain specific and may participate in
HS-dependent neurobiologic events (5). HS3ST4 can generate tetrasulfated
heparan sulfate disaccharide, the most highly sulfated sugar found in
biological samples (6, 7), and may have a role in assisting HSV-1 entry
and spread (8). HS3ST4 is a Golgi resident type II membrane protein and
has the longest proline rich stem region among all HS3STs (3, 5).
Bernfield, M. et al. (1999) Annu. Rev.
Esko, J.D. and Selleck, S.B. (2002) Annu. Rev. Biochem.
Shworak, N.W. et al. (1999) J. Biol.
Xu, D. et al. (2005) Biochem. J.
Lawrence, R. et al. (2007) Matrix Biol.
Mochizuki, H. et al. (2003) J. Biol.
Wu, Z.L. et al. (2004) J. Biol. Chem.
Tiwari,V. et al. (2005) Biochem. Biophys.
Res. Commun. 338:930.
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