Human/Mouse HMGA2 Antibody

(6 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse HMGA2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human HMGB1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human HMGA2
    Ser2-Asp109
    Accession # P52926
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    Immersion fixed SCC-25 human squamous cell sarcoma cell line
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human and Mouse HMGA2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, MDA‑MD‑231 human breast cancer cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and P19 mouse embryonal carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse HMGA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3184) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HMGA2 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Mouse HMGA2 by Simple WesternTM. Simple Western lane view shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HMGA2 at approximately 30 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse HMGA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3184) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HMGA2
HMGA2, previously known as HMGI-C, belongs to the group of high mobility chromosomal proteins that have the AT-hook DNA-binding motif. It is highly and ubiquitously expressed during embryogenesis. Chromosomal rearrangements of the HMGA2 gene and overexpression are frequently associated with benign and malignant tumors, respectively. Human and mouse HMGA2 share 95% amino acid sequence identity.
  • Long Name:
    High Mobility Group AT-hook 2
  • Entrez Gene IDs:
    8091 (Human)
  • Alternate Names:
    BABL; BABLHMGI-C; high mobility group AT-hook 2; High mobility group AT-hook protein 2; high mobility group protein HMGI-C; high-mobility group (nonhistone chromosomal) protein isoform I-C; High-mobility group protein HMGI-C; HMGA2; HMGIC; HMGICSTQTL9; LIPO
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. MiRNA let-7g regulates skeletal myoblast motility via Pinch-2.
    Authors: Boudoukha S, Rivera Vargas T, Dang I, Kropp J, Cuvellier S, Gautreau A, Polesskaya A
    FEBS Lett, 2014;588(9):1623-9.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. Generation and characterisation of a canine EGFP-HMGA2 prostate cancer in vitro model.
    Authors: Willenbrock, Saskia, Wagner, Siegfrie, Reimann-Berg, Nicola, Moulay, Mohammed, Hewicker-Trautwein, Marion, Nolte, Ingo, Murua Escobar, Hugo
    PLoS ONE, 2014;9(6):e98788.
    Species: Canine
    Sample Type: Whole Cells
    Application: IHC Paraffin-embedded
  3. HMGA1 and HMGA2 expression and comparative analyses of HMGA2, Lin28 and let-7 miRNAs in oral squamous cell carcinoma.
    Authors: Sterenczak K, Eckardt A, Kampmann A, Willenbrock S, Eberle N, Langer F, Kleinschmidt S, Hewicker-Trautwein M, Kreipe H, Nolte I, Murua Escobar H, Gellrich N
    BMC Cancer, 2014;14(0):694.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  4. Downregulation of HMGA2 by the pan-deacetylase inhibitor panobinostat is dependent on hsa-let-7b expression in liver cancer cell lines.
    Exp Cell Res, 2012;318(15):1832-43.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  5. Subsets of very low risk wilms tumor show distinctive gene expression, histologic, and clinical features.
    Authors: Sredni ST, Gadd S, Huang CC, Breslow N, Grundy P, Green DM, Dome JS, Shamberger RC, Beckwith JB, Perlman EJ,
    Clin. Cancer Res., 2009;15(22):6800-9.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  6. Clinical significance of high mobility group A2 in human gastric cancer and its relationship to let-7 microRNA family.
    Authors: Motoyama K, Inoue H, Nakamura Y, Uetake H, Sugihara K, Mori M
    Clin. Cancer Res., 2008;14(8):2334-40.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
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