Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Western Blot, Simple Western

Cited:

Immunohistochemistry, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all HO-1/HMOX1/HSP32 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HO‑1/HMOX1/HSP32
Met1-Thr261
Accession # P09601

Specificity

Detects human and mouse HO‑1/HMOX1/HSP32 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human HO-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse HO‑1/HMOX1/HSP32 Antibody

Detection of Human/Mouse HO-1/HMOX1/ HSP32 antibody by Western Blot.

Detection of Human/Mouse HO‑1/HMOX1/ HSP32 by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line, DU145 human prostate carcinoma cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse HO-1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3776) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human HO-1 and HO-2 (5 ng/lane) were included. A specific band for HO-1/HMOX1/HSP32 was detected at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human and Mouse HO‑1/HMOX1/HSP32 antibody by Simple WesternTM.

Detection of Human and Mouse HO‑1/HMOX1/HSP32 by Simple WesternTM.

Simple Western lane view shows lysates of A20 mouse B cell lymphoma cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for HO-1/HMOX1/HSP32 at approximately 37 kDa (as indicated) using 25 µg/mL of Goat Anti-Human/Mouse HO-1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3776) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western Blot Shows Human HO-1/HMOX1/HSP32 Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human HO‑1/HMOX1/HSP32 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HO-1/HMOX1/HSP32 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse HO-1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3776) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse HO‑1/HMOX1/HSP32 Antibody

Application
Recommended Usage

Knockout Validated

HO‑1/HMOX1/HSP32 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in HO‑1/HMOX1/HSP32 knockout HeLa cell line.

Simple Western

25 µg/mL
Sample: A20 mouse B cell lymphoma cell line and A549 human lung carcinoma cell line

Western Blot

0.5 µg/mL
Sample: A549 human lung carcinoma cell line, DU145 human prostate carcinoma cell line, and A20 mouse B cell lymphoma cell line

Reviewed Applications

Read 1 review rated 5 using AF3776 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HO-1/HMOX1/HSP32

Heme Oxygenase 1 (HO-1), also known as HMOX1 and Heat Shock Protein 32 (HSP32), is a 32 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 (HO-1/HMOX1) and a constitutive heme oxygenase-2 (HO-2/HMOX2). HO-1 expression is induced by heme and other non-heme compounds. Human HO-1 shares 82% amino acid sequence identity with mouse HO-1.

Long Name

Heme Oxygenase 1

Alternate Names

HMOX1, HO1, HSP32

Entrez Gene IDs

3162 (Human); 24451 (Mouse); 15368 (Rat)

Gene Symbol

HMOX1

UniProt

P09601

Additional HO-1/HMOX1/HSP32 Products

Product Documents for Human/Mouse HO‑1/HMOX1/HSP32 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse HO‑1/HMOX1/HSP32 Antibody

For research use only

Citations for Human/Mouse HO‑1/HMOX1/HSP32 Antibody

Customer Reviews for Human/Mouse HO‑1/HMOX1/HSP32 Antibody (1)

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  • HO-1 in A549
    Name: Anonymous
    Application: Simple Western
    Sample Tested: A549 human lung carcinoma cell line
    Species: Human
    Verified Customer | Posted 08/07/2024
    HO-1 in A549 after cigarette smoke treatment
    Milk-free diluent, 1:40
    Human/Mouse HO‑1/HMOX1/HSP32 Antibody AF3776

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