Human/Mouse HO-1/HMOX1/HSP32 Antibody

  (2 citations)     
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  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse HO‑1/HMOX1/HSP32 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human HO-2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human HO‑1/HMOX1/HSP32
    Met1-Thr261
    Accession # P09601
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    25 µg/mL
    See below
  • Knockout Validated
    HO‑1/HMOX1/HSP32 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in HO‑1/HMOX1/HSP32 knockout HeLa cell line.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human/Mouse HO‑1/HMOX1/
HSP32 by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line, DU145 human prostate carcinoma cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse HO‑1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3776) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human HO-1 and HO-2 (5 ng/lane) were included. A specific band for HO-1/HMOX1/HSP32 was detected at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human and Mouse HO‑1/HMOX1/HSP32 by Simple WesternTM. Simple Western lane view shows lysates of A20 mouse B cell lymphoma cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for HO‑1/HMOX1/HSP32 at approximately 37 kDa (as indicated) using 25 µg/mL of Goat Anti-Human/Mouse HO‑1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3776) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Knockout Validated
Western Blot Shows Human HO‑1/HMOX1/HSP32 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HO-1/HMOX1/HSP32 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse HO‑1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3776) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HO‑1/HMOX1/HSP32 at approximately 32 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HO-1/HMOX1/HSP32

Heme Oxygenase 1 (HO-1), also known as HMOX1 and Heat Shock Protein 32 (HSP32), is a 32 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 (HO-1/HMOX1) and a constitutive heme oxygenase-2 (HO-2/HMOX2). HO-1 expression is induced by heme and other non-heme compounds. Human HO-1 shares 82% amino acid sequence identity with mouse HO-1.

  • Long Name:
    Heme Oxygenase 1
  • Entrez Gene IDs:
    3162 (Human); 24451 (Mouse); 15368 (Rat)
  • Alternate Names:
    bK286B10; EC 1.14.99.3; heat shock protein, 32-kD; heme oxygenase (decycling) 1; HMOX1; HO; HO1; HO-1; HO-1heme oxygenase 1; HSP32
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Red Yeast Rice Protects Circulating Bone Marrow-Derived Proangiogenic Cells against High-Glucose-Induced Senescence and Oxidative Stress: The Role of Heme Oxygenase-1
    Authors: JT Liu, HY Chen, WC Chen, KM Man, YH Chen
    Oxid Med Cell Longev, 2017;2017(0):3831750.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Anti-inflammatory effects of ethyl acetate fraction from Melilotus suaveolens Ledeb on LPS-stimulated RAW 264.7 cells.
    Authors: Tao JY, Zheng GH, Zhao L, Wu JG, Zhang XY, Zhang SL, Huang ZJ, Xiong FL, Li CM
    J Ethnopharmacol, 2009;123(1):97-105.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB

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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 203  
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB , Simple Western HAF109 20  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL003 6
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 7  
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB , ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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