LPP Antibody (691121)

R&D Systems | Catalog # MAB6927

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 691121
View all LPP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human LPP
Lys138-Gln261
Accession # Q93052

Specificity

Detects human LPP in direct ELISAs.  In direct ELISAs, no cross-reactivity with recombinant human TRIP-4, -6, or -11 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for LPP Antibody (691121)

Detection of Human and Mouse LPP antibody by Western Blot.

Detection of Human and Mouse LPP by Western Blot.

Western blot shows lysates of MDA-MB-468 human breast cancer cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human LPP Monoclonal Antibody (Catalog # MAB6927) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LPP at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for LPP Antibody (691121)

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: MDA‑MB‑468 human breast cancer cell line and C2C12 mouse myoblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LPP

Human LIM domain-containing preferred translocation partner in lipoma (LPP) is an 80 kDa intracellular protein that contains an N-terminal Pro-rich region (aa 41-370) followed by three tandem LIM domains (aa 414-603). It localizes to focal adhesion plaques where it regulates junction assembly and cytoskeleton remodeling through interactions with a-Actinin, VASP, SCRIB, Supervillin, and Palladin. In the nucleus, LPP coactivates the transcription factor PEA3 and protects telomeres from DNA damage. LPP is a frequent target of chromosomal translocations with HMG-A2 in lipoma. Within aa 138-261, human LPP shares 90% and 77% aa sequence identity with mouse and rat LPP, respectively.

Long Name

Lipoma-Preferred Partner

Alternate Names

DKFZp779O0231, FLJ30652, FLJ41512, LIM domain containing preferred translocation partner in lipoma, LIM domain-containing preferred translocation partner in lipoma, LIM protein, lipoma-preferred partner

Entrez Gene IDs

4026 (Human); 210126 (Mouse); 288010 (Rat)

Gene Symbol

LPP

UniProt

Q93052

Additional LPP Products

Product Documents for LPP Antibody (691121)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for LPP Antibody (691121)

For research use only

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