Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 691121
View all LPP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human LPP
Lys138-Gln261
Accession # Q93052

Specificity

Detects human LPP in direct ELISAs.  In direct ELISAs, no cross-reactivity with recombinant human TRIP-4, -6, or -11 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse LPP Antibody

Detection of Human and Mouse LPP antibody by Western Blot.

Detection of Human and Mouse LPP by Western Blot.

Western blot shows lysates of MDA-MB-468 human breast cancer cell line and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human LPP Monoclonal Antibody (Catalog # MAB6927) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LPP at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse LPP Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: MDA‑MB‑468 human breast cancer cell line and C2C12 mouse myoblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LPP

Human LIM domain-containing preferred translocation partner in lipoma (LPP) is an 80 kDa intracellular protein that contains an N-terminal Pro-rich region (aa 41-370) followed by three tandem LIM domains (aa 414-603). It localizes to focal adhesion plaques where it regulates junction assembly and cytoskeleton remodeling through interactions with a-Actinin, VASP, SCRIB, Supervillin, and Palladin. In the nucleus, LPP coactivates the transcription factor PEA3 and protects telomeres from DNA damage. LPP is a frequent target of chromosomal translocations with HMG-A2 in lipoma. Within aa 138-261, human LPP shares 90% and 77% aa sequence identity with mouse and rat LPP, respectively.

Long Name

Lipoma-Preferred Partner

Alternate Names

DKFZp779O0231, FLJ30652, FLJ41512, LIM domain containing preferred translocation partner in lipoma, LIM domain-containing preferred translocation partner in lipoma, LIM protein, lipoma-preferred partner

Entrez Gene IDs

4026 (Human); 210126 (Mouse); 288010 (Rat)

Gene Symbol

LPP

UniProt

Q93052

Additional LPP Products

Product Documents for Human/Mouse LPP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse LPP Antibody

For research use only

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