Human/Mouse MafK Antibody

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Detection of Human/Mouse MafK by Western Blot.
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Product Details
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Human/Mouse MafK Antibody Summary

Species Reactivity
Human, Mouse
Detects human and mouse MafK in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MafB or rhMafF is observed. In Western blots, prolonged film exposures may slightly detect rhMafG.
Monoclonal Mouse IgG2B Clone # 381923
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human MafK
Accession # O60675
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse MafK antibody by Western Blot. View Larger

Detection of Human/Mouse MafK by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL Mouse Anti-Human/Mouse MafK Monoclonal Antibody (Catalog # MAB3809) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MafB, MafF, MafG, and MafK (0.5 ng/lane) were included. A specific band for MafK was detected at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MafK

Maf family members form a unique subclass of basic-leucine zipper (bZIP) transcription factors. Maf proteins are subdivided into two groupings: large, including c‑Maf, Nrl, MafA, and MafB; and small, including MafF, MafG, and MafK, also known as p18. Large Mafs contain an N-terminal acidic domain important for transcriptional activation that is lacking in small Maf family members. Heterodimers of MafK and p45 NF-E2, another bZIP factor, promote globin gene expression and erythroid differentiation in erythroleukemia cells.

Long Name
v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog K
Entrez Gene IDs
7975 (Human); 17135 (Mouse)
Alternate Names
FLJ32205; MafK; MGC71717; NFE2U; transcription factor MafK; ubiquitous (p18); v-maf avian musculoaponeurotic fibrosarcoma oncogene family, protein K; v-maf musculoaponeurotic fibrosarcoma (avian) oncogene family, protein K; v-maf musculoaponeurotic fibrosarcoma oncogene homolog K (avian)

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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