MafK Antibody (381923)

R&D Systems | Catalog # MAB3809

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 381923
View all MafK Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MafK
Thr2-Ser156
Accession # O60675

Specificity

Detects human and mouse MafK in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MafB or rhMafF is observed. In Western blots, prolonged film exposures may slightly detect rhMafG.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for MafK Antibody (381923)

Detection of Human/Mouse MafK antibody by Western Blot.

Detection of Human/Mouse MafK by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL Mouse Anti-Human/Mouse MafK Monoclonal Antibody (Catalog # MAB3809) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MafB, MafF, MafG, and MafK (0.5 ng/lane) were included. A specific band for MafK was detected at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for MafK Antibody (381923)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: A549 human lung carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MafK

Maf family members form a unique subclass of basic-leucine zipper (bZIP) transcription factors. Maf proteins are subdivided into two groupings: large, including c‑Maf, Nrl, MafA, and MafB; and small, including MafF, MafG, and MafK, also known as p18. Large Mafs contain an N-terminal acidic domain important for transcriptional activation that is lacking in small Maf family members. Heterodimers of MafK and p45 NF-E2, another bZIP factor, promote globin gene expression and erythroid differentiation in erythroleukemia cells.

Long Name

v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog K

Alternate Names

NFE2U

Entrez Gene IDs

7975 (Human); 17135 (Mouse)

Gene Symbol

MAFK

UniProt

O60675

Additional MafK Products

Product Documents for MafK Antibody (381923)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for MafK Antibody (381923)

For research use only

Citations for MafK Antibody (381923)

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