Human/Mouse MafK Antibody

Catalog # Availability Size / Price Qty
MAB3809
MAB3809-SP
Detection of Human/Mouse MafK by Western Blot.
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Human/Mouse MafK Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse MafK in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MafB or rhMafF is observed. In Western blots, prolonged film exposures may slightly detect rhMafG.
Source
Monoclonal Mouse IgG2B Clone # 381923
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human MafK
Thr2-Ser156
Accession # O60675
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human/Mouse MafK by Western Blot. View Larger

Detection of Human/Mouse MafK by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL Mouse Anti-Human/Mouse MafK Monoclonal Antibody (Catalog # MAB3809) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MafB, MafF, MafG, and MafK (0.5 ng/lane) were included. A specific band for MafK was detected at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MafK

Maf family members form a unique subclass of basic-leucine zipper (bZIP) transcription factors. Maf proteins are subdivided into two groupings: large, including c‑Maf, Nrl, MafA, and MafB; and small, including MafF, MafG, and MafK, also known as p18. Large Mafs contain an N-terminal acidic domain important for transcriptional activation that is lacking in small Maf family members. Heterodimers of MafK and p45 NF-E2, another bZIP factor, promote globin gene expression and erythroid differentiation in erythroleukemia cells.

Long Name
v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog K
Entrez Gene IDs
7975 (Human); 17135 (Mouse)
Alternate Names
FLJ32205; MafK; MGC71717; NFE2U; transcription factor MafK; ubiquitous (p18); v-maf avian musculoaponeurotic fibrosarcoma oncogene family, protein K; v-maf musculoaponeurotic fibrosarcoma (avian) oncogene family, protein K; v-maf musculoaponeurotic fibrosarcoma oncogene homolog K (avian)

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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