Detects human and mouse MafK in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MafB or rhMafF is observed. In Western blots, prolonged film exposures may slightly detect rhMafG.
Monoclonal Mouse IgG2B Clone # 381923
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human MafK Thr2-Ser156 Accession # O60675
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human/Mouse MafK by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL Mouse Anti-Human/Mouse MafK Monoclonal Antibody (Catalog # MAB3809) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MafB, MafF, MafG, and MafK (0.5 ng/lane) were included. A specific band for MafK was detected at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Maf family members form a unique subclass of basic-leucine zipper (bZIP) transcription factors. Maf proteins are subdivided into two groupings: large, including c‑Maf, Nrl, MafA, and MafB; and small, including MafF, MafG, and MafK, also known as p18. Large Mafs contain an N-terminal acidic domain important for transcriptional activation that is lacking in small Maf family members. Heterodimers of MafK and p45 NF-E2, another bZIP factor, promote globin gene expression and erythroid differentiation in erythroleukemia cells.
v-maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog K
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