Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 85340
View all nNOS Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human nNOS
Ser218-Ser1434
Accession # P29475

Specificity

Detects human nNos in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse nNOS Antibody

Detection of Human and Mouse nNOS antibody by Western Blot.

Detection of Human and Mouse nNOS by Western Blot.

Western blot shows lysates ofSf21S. frugiperdainsect ovarian cell line either mock transfected or transfected with human nNOS, and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse nNOS Monoclonal Antibody (Catalog # MAB24161) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for nNOS at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
nNOS antibody in Neuro-2A Mouse Cell Line by Immunocytochemistry (ICC).

nNOS in Neuro‑2A Mouse Cell Line.

nNOS was detected in immersion fixed Neuro-2A mouse neuroblastoma cell line using Mouse Anti-Human/Mouse nNOS Monoclonal Antibody (Catalog # MAB24161) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei, cytoplasm and cell membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of nNOS antibody in Neuro-2A Mouse Cell Line antibody by Flow Cytometry.

Detection of nNOS in Neuro‑2A Mouse Cell Line by Flow Cytometry.

Neuro-2A mouse neuroblastoma cell line was stained with Mouse Anti-Human/Mouse nNOS Monoclonal Antibody (Catalog # MAB24161, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse nNOS Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Neuro-2A mouse neuroblastoma cell line

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: Neuro‑2A mouse neuroblastoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Western Blot

1 µg/mL
Sample: Sf 21 S. frugiperda insect ovarian cell line transfected with human nNOS and mouse brain tissue

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: nNOS

nNOS is one of three NOS enzymes that catalyze the oxidation of L-argine to L-citruline and nitric oxide. nNOS exists as homodimers containing a cytochrome P450‑like prosthetic heme group in the N-terminal half. It also has a tightly bound FAD and FMN group in the C-terminal half. At least 4 isoforms of human nNOS are known. Human nNOS shares about 55% amino acid sequence identity with eNOS and iNOS. It also shares 96% sequence identity with mouse or rat nNOS.

Long Name

Neuronal Nitric Oxide Synthase

Alternate Names

NOS1

Entrez Gene IDs

4842 (Human); 18125 (Mouse)

Gene Symbol

NOS1

UniProt

P29475

Additional nNOS Products

Product Documents for Human/Mouse nNOS Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse nNOS Antibody

For research use only

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Protocols

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