Human/Mouse PKA RII alpha Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8000-100
MAB8000-SP
Detection  of Human PKA RII alpha  by Western Blot.
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Human/Mouse PKA RII alpha Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human PKA RII alpha in direct ELISAs. Detects human and mouse PKA RII alpha in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2394D
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Synthetic peptide containing Human/Mouse PKA RII alpha
Accession # P13861
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
0.3-25 µg/mL
Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human PKA RII alpha  by Western Blot. View Larger

Detection of Human PKA RII alpha by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and PC‑3 human prostate cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse PKA RII alpha Monoclonal Antibody (Catalog # MAB8000) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PKA RII alpha at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry PKA RII alpha   in MDA-MB-231 Human Cell Line. View Larger

PKA RII alpha in MDA-MB-231 Human Cell Line. PKA RII alpha was detected in immersion fixed MDA‑MB‑231 human breast cancer cell line using Rabbit Anti-Human/Mouse PKA RII alpha Monoclonal Antibody (Catalog # MAB8000) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry PKA RII alpha   in Human Pancreatic Cancer Tissue. View Larger

PKA RII alpha in Human Pancreatic Cancer Tissue. PKA RII alpha was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Rabbit Anti-Human/Mouse PKA RII alpha Monoclonal Antibody (Catalog # MAB8000) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKA RII alpha

PRKAR2 is a regulatory subunit of the cAMP-dependent protein kinases involved in CAMP signaling in cells.  Four types of regulatory chains are found and their expression varies among tissues.  In some cases expression is constitutive and in other cases it is inducible.  PRKAR2 seems to be involved in both endosome-to-Golgi and Golgi-to-ER transport.

Long Name
cAMP-dependent Protein Kinase Regulatory Type II alpha
Entrez Gene IDs
5576 (Human); 19087 (Mouse); 29699 (Rat)
Alternate Names
PKA RII alpha; PRKAR2A

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

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