Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2394D
View all PKA RII alpha Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing Human/Mouse PKA RII alpha
Accession # P13861

Specificity

Detects human PKA RII alpha in direct ELISAs. Detects human and mouse PKA RII alpha in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse PKA RII alpha Antibody

Detection of Human PKA RIIa antibody by Western Blot.

Detection of Human PKA RII alpha by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse PKA RIIa Monoclonal Antibody (Catalog # MAB8000) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PKA RIIa at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PKA RIIa antibody in MDA-MB-231 Human Cell Line by Immunocytochemistry (ICC).

PKA RII alpha in MDA-MB-231 Human Cell Line.

PKA RIIa was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Rabbit Anti-Human/Mouse PKA RIIa Monoclonal Antibody (Catalog # MAB8000) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
PKA RIIa antibody in Human Pancreatic Cancer Tissue by Immunocytochemistry (ICC).

PKA RII alpha in Human Pancreatic Cancer Tissue.

PKA RIIa was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Rabbit Anti-Human/Mouse PKA RIIa Monoclonal Antibody (Catalog # MAB8000) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human/Mouse PKA RII alpha Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed MDA-MB-231 human breast cancer cell line

Immunohistochemistry

0.3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue

Western Blot

0.5 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and PC‑3 human prostate cancer cell line

Reviewed Applications

Read 1 review rated 5 using MAB8000 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PKA RII alpha

PRKAR2 is a regulatory subunit of the cAMP-dependent protein kinases involved in CAMP signaling in cells.  Four types of regulatory chains are found and their expression varies among tissues.  In some cases expression is constitutive and in other cases it is inducible.  PRKAR2 seems to be involved in both endosome-to-Golgi and Golgi-to-ER transport.

Long Name

cAMP-dependent Protein Kinase Regulatory Type II alpha

Alternate Names

PRKAR2A

Entrez Gene IDs

5576 (Human); 19087 (Mouse); 29699 (Rat)

Gene Symbol

PRKAR2A

UniProt

P13861

Additional PKA RII alpha Products

Product Documents for Human/Mouse PKA RII alpha Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse PKA RII alpha Antibody

For research use only

Citations for Human/Mouse PKA RII alpha Antibody

Customer Reviews for Human/Mouse PKA RII alpha Antibody (1)

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  • Human/Mouse PKA RII alpha Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Adult lung
    Species: Mouse
    Verified Customer | Posted 11/04/2019
    Human/Mouse PKA RII alpha Antibody MAB8000

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Protocols

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