Human/Mouse PKA RII alpha Antibody Summary
Accession # P13861
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human PKA RII alpha by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and PC‑3 human prostate cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse PKA RII alpha Monoclonal Antibody (Catalog # MAB8000) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PKA RII alpha at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PKA RII alpha in MDA-MB-231 Human Cell Line. PKA RII alpha was detected in immersion fixed MDA‑MB‑231 human breast cancer cell line using Rabbit Anti-Human/Mouse PKA RII alpha Monoclonal Antibody (Catalog # MAB8000) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
PKA RII alpha in Human Pancreatic Cancer Tissue. PKA RII alpha was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Rabbit Anti-Human/Mouse PKA RII alpha Monoclonal Antibody (Catalog # MAB8000) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKA RII alpha
PRKAR2 is a regulatory subunit of the cAMP-dependent protein kinases involved in CAMP signaling in cells. Four types of regulatory chains are found and their expression varies among tissues. In some cases expression is constitutive and in other cases it is inducible. PRKAR2 seems to be involved in both endosome-to-Golgi and Golgi-to-ER transport.
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Cell and Tissue Staining Kits
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