Detection of Human C‑Reactive Protein/CRP by Western Blot. Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Porcine C‑Reactive Protein/CRP Monoclonal Antibody (Catalog # MAB1707) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for C‑Reactive Protein/CRP at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: C-Reactive Protein/CRP
CRP is a secreted protein that belongs to the Pentraxin family. It
circulates as a non-covalent homopentamer. The CRP concentration in
human plasma is highly increased during acute phase response to tissue
injury or infection.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.