Human/Mouse Preso/FRMPD4 Antibody Summary
Accession # Q14CM0
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Mouse Preso/FRMPD4 by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Preso/FRMPD4 Monoclonal Antibody (Catalog # MAB7676) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Preso/FRMPD4 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Preso/FRMPD4 by Simple WesternTM. Simple Western lane view shows lysates of bEnd.3 mouse endothelioma cell line, loaded at 0.5 mg/mL. A specific band was detected for Preso/FRMPD4 at approximately 170 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse Preso/FRMPD4 Monoclonal Antibody (Catalog # MAB7676). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Preso, also known as FRMPD4 and PDZD10, is a scaffolding protein that is widely expressed in the brain as 140 kDa and 170 kDa species. It contains one WW domain (aa 33-66) one PDZ domain (aa 78-155), and one FERM domain (aa 204-519). The 140 kDa species is enriched in post-synaptic densities where it interacts with PSD-95, SAP97, SAP102, and F-actin. Preso promotes dendritic spine extension and maintenance as well as excitatory synaptic transmission. Within aa 890-1022, human Preso shares approximately 94% aa sequence identity with mouse and rat Preso.
Citation for Human/Mouse Preso/FRMPD4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Preso regulates NMDA receptor-mediated excitotoxicity via modulating nitric oxide and calcium responses after traumatic brain injury
Authors: P Luo, X Li, X Wu, S Dai, Y Yang, H Xu, D Jing, W Rao, H Xu, X Gao, Z Fei, H Lu
Cell Death Dis, 2019;10(7):496.
Sample Types: Cell Lysates
Applications: Western Blot
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human/Mouse Preso/FRMPD4 Antibody
There are currently no reviews for this product. Be the first to review Human/Mouse Preso/FRMPD4 Antibody and earn rewards!
Have you used Human/Mouse Preso/FRMPD4 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image