Detection of Human and Mouse Preso/FRMPD4 by Western Blot. Western blot shows lysates of IMR‑32 human neuroblastoma cell line and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Preso/FRMPD4 Monoclonal Antibody (Catalog # MAB7676) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Preso/FRMPD4 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Preso/FRMPD4 by Simple WesternTM. Simple Western lane view shows lysates of bEnd.3 mouse endothelioma cell line, loaded at 0.5 mg/mL. A specific band was detected for Preso/FRMPD4 at approximately 170 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse Preso/FRMPD4 Monoclonal Antibody (Catalog # MAB7676). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Preso, also known as FRMPD4 and PDZD10, is
a scaffolding protein that is widely expressed in the brain as 140 kDa and 170
kDa species. It contains one WW domain (aa 33-66) one PDZ domain (aa 78-155),
and one FERM domain (aa 204-519). The 140 kDa species is enriched in
post-synaptic densities where it interacts with PSD-95, SAP97, SAP102, and
F-actin. Preso promotes dendritic spine extension and maintenance as well as
excitatory synaptic transmission. Within aa 890-1022, human Preso shares
approximately 94% aa sequence identity with mouse and rat Preso.
FERM and PDZ Domain Containing 4
Entrez Gene IDs:
9758 (Human); 333605 (Mouse); 302656 (Rat)
FERM and PDZ domain containing 4; FERM and PDZ domain-containing protein 4; FRMPD4; MGC142260; MGC142262; PDZ domain containing 10; PDZD10; PDZK10
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