Human/Mouse PSMB7 Antibody Summary
Accession # Q99436
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Mouse PSMB7 by Western Blot. Western blot shows lysates of 786-O human renal cell adenocarcinoma cell line, beta TC-6 mouse beta cell insulinoma cell line, AR42J human acinar pancreatic cancer cell line, HepG2 human hepatocellular carcinoma cell line, Hepa 1-6 mouse hepatoma cell line, MCF-7 human breast cancer cell line, and NMuMG mouse mammary gland epithelial cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human PSMB7 Monoclonal Antibody (Catalog # MAB7590) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PSMB7 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
PSMB7 (proteasome subunit, beta-type 7), also called proteasome subunit Z, is a widely expressed, non-catalytic component of the 20S proteasome core within the 26S proteasome that stabilizes late stages of proteasome assembly. Human PSMB7 is synthesized as a 277 amino acid (aa) protein with a 43 aa propeptide and a 234 active protein that contains one potential tyrosine phosphorylation site. Active human PSMB7 shares 97% aa sequence identity with mouse and rat PSMB7. Total PSMB7 expression is positively correlated with chemotherapy resistance in human breast cancer.
Citation for Human/Mouse PSMB7 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
A cryptic K48 ubiquitin chain binding site on UCH37 is required for its role in proteasomal degradation
Authors: J Du, S Babik, Y Li, KK Deol, SJ Eyles, J Fejzo, M Tonelli, E Strieter
Sample Types: Cell Lysates
Applications: Western Blot
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