Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 787807
View all PSMB7 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PSMB7
Thr44-Ser277
Accession # Q99436

Specificity

Detects human PSMB7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human PSMB10 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse PSMB7 Antibody

Detection of Human and Mouse PSMB7 antibody by Western Blot.

Detection of Human and Mouse PSMB7 by Western Blot.

Western blot shows lysates of 786-O human renal cell adenocarcinoma cell line, beta TC-6 mouse beta cell insulinoma cell line, AR42J human acinar pancreatic cancer cell line, HepG2 human hepatocellular carcinoma cell line, Hepa 1-6 mouse hepatoma cell line, MCF-7 human breast cancer cell line, and NMuMG mouse mammary gland epithelial cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human PSMB7 Monoclonal Antibody (Catalog # MAB7590) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PSMB7 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse PSMB7 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: 786‑O human renal cell adenocarcinoma cell line, MCF‑7 human breast cancer cell line, and HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PSMB7

PSMB7 (proteasome subunit, beta-type 7), also called proteasome subunit Z, is a widely expressed, non-catalytic component of the 20S proteasome core within the 26S proteasome that stabilizes late stages of proteasome assembly. Human PSMB7 is synthesized as a 277 amino acid (aa) protein with a 43 aa propeptide and a 234 active protein that contains one potential tyrosine phosphorylation site. Active human PSMB7 shares 97% aa sequence identity with mouse and rat PSMB7. Total PSMB7 expression is positively correlated with chemotherapy resistance in human breast cancer.

Long Name

Proteasome (Prosome, Macropain) Subunit, beta Type, 7

Alternate Names

Macropain Chain Z, Proteasome Subunit Z

Entrez Gene IDs

5695 (Human); 19177 (Mouse); 85492 (Rat)

Gene Symbol

PSMB7

UniProt

Q99436 (Human)

Additional PSMB7 Products

Product Documents for Human/Mouse PSMB7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse PSMB7 Antibody

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Human/Mouse PSMB7 Antibody

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