Human/Mouse/Rat alpha -Actinin 1 Antibody

• Species Reactivity
  Human, Mouse, Rat
• Specificity
  Detects human, mouse and rat alpha -Actinin 1 in Western blots.
• Source
  Polyclonal Goat IgG
• Purification
  Antigen Affinity-purified
• Immunogen
  E. coli-derived recombinant human alpha -Actinin 1
  Asp272-Thr500
  Accession # P12814
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  0.5 µg/mL
  See below
• Immunocytochemistry
  5-15 µg/mL
  See below

Western Blot click image to view larger

Detection of Human, Mouse, and Rat alpha ‑Actinin 1 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat alpha -Actinin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8279) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for alpha -Actinin 1 at approximately 100-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry click image to view larger

alpha -Actinin 1 in A172 Human Cell Line. alpha -Actinin 1 was detected in immersion fixed A172 human glioblastoma cell line using Goat Anti-Human/Mouse/Rat alpha -Actinin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8279) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reconstitution
  Reconstitute at 0.2 mg/mL in sterile PBS.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• Long Name:
  Actinin alpha 1

• Entrez Gene IDs:
  87 (Human); 109711 (Mouse); 81634 (Rat)

• Alternate Names:
  actinin 1 smooth muscle; actinin, alpha 1; ACTN1; alpha Actinin 1; alphaActinin 1; alpha-Actinin 1; Alpha-actinin cytoskeletal isoform; alpha-actinin-1; BDPLT15; F-actin cross-linking protein; FLJ40884; FLJ54432; Non-muscle alpha-actinin-1

FAQs

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