Human/Mouse/Rat alpha -Actinin 1 Antibody

AF8279 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF8279
AF8279-SP

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Detection of Human, Mouse, and Rat alpha ‑Actinin 1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat alpha -Actinin 1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat alpha -Actinin 1 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human alpha -Actinin 1
Asp272-Thr500
Accession # P12814

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot

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Detection of Human, Mouse, and Rat alpha ‑Actinin 1 by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat alpha -Actinin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8279) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for alpha -Actinin 1 at approximately 100-105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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alpha -Actinin 1 in A172 Human Cell Line.

alpha -Actinin 1 was detected in immersion fixed A172 human glioblastoma cell line using Goat Anti-Human/Mouse/Rat alpha -Actinin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8279) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha Actinin 1

Alpha-Actinin 1 (ACTN1) is a member of the spectrin superfamily with a predicted molecular weight of approximately 100 kDa. It is 892 amino acids in length and shares 99% aa identity with mouse and rat ACTN1. Multiple splice forms exist, resulting in 4 distinct isoforms with different tissue expression patterns. ACTN1 binds to F-actin as well as other cytoskeletal and signaling proteins and plays an important role in both cell motility and muscle contraction.

Long Name

Actinin alpha 1

Entrez Gene IDs

87 (Human); 109711 (Mouse); 81634 (Rat)

Alternate Names

actinin 1 smooth muscle; actinin, alpha 1; ACTN1; alpha Actinin 1; alphaActinin 1; alpha-Actinin 1; Alpha-actinin cytoskeletal isoform; alpha-actinin-1; BDPLT15; F-actin cross-linking protein; FLJ40884; FLJ54432; Non-muscle alpha-actinin-1