Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 666316
View all Annexin A2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Annexin A2
Met1-Asp339
Accession # P07355

Specificity

Detects human Annexin A2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 25% cross-reactivity with recombinant human (rh) Annexin A1, A7, A9, A11, and A13 is observed. No cross-reactivity with rhAnnexin A3, A4, A6, A8, or A10 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat Annexin A2 Antibody

Detection of Human, Mouse, and Rat Annexin A2 antibody by Western Blot.

Detection of Human, Mouse, and Rat Annexin A2 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat Annexin A2 Monoclonal Antibody (Catalog # MAB3928) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Annexin A2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Annexin A2 antibody in Human Liver by Immunohistochemistry (IHC-P).

Annexin A2 in Human Liver.

Annexin A2 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human/Mouse/Rat Annexin A2 Monoclonal Antibody (Catalog # MAB3928) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes and cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Annexin A2 antibody by Simple WesternTM.

Detection of Human Annexin A2 by Simple WesternTM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Annexin A2 at approximately 43 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat Annexin A2 Monoclonal Antibody (Catalog # MAB3928). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat Annexin A2 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Simple Western

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line

Western Blot

0.1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line

Reviewed Applications

Read 2 reviews rated 4 using MAB3928 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Annexin A2

Annexin A2 (ANXA2), also known as Annexin II and Lipocortin II (LPC2), is an approximately 40 kDa member of the Annexin family of calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. Phosphorylation of Annexin A2 at Tyr23 regulates its involvement in endosomal trafficking and actin cytoskeleton rearrangement. The Annexins consist of a unique amino terminal domain followed by a homologous C‑terminal core domain containing the calcium-dependent phospholipid-binding sites. The C‑terminal domain is comprised of four 60-70 amino acid (aa) annexin repeats. Annexin A2 also functions as an autocrine factor to enhance osteoclast formation and bone resorption and is a major cellular substrate of the tyrosine kinase Src. Human Annexin A2 shares 97% identity with mouse and rat Annexin A2.

Alternate Names

ANX2L4, ANXA2, CAL1H, LIP2, Lipocortin-2, LPC2D, PAP-IV

Entrez Gene IDs

302 (Human); 12306 (Mouse); 56611 (Rat)

Gene Symbol

ANXA2

UniProt

P07355 (Human)

Additional Annexin A2 Products

Product Documents for Human/Mouse/Rat Annexin A2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Annexin A2 Antibody

For research use only

Citations for Human/Mouse/Rat Annexin A2 Antibody

Customer Reviews for Human/Mouse/Rat Annexin A2 Antibody (2)

4 out of 5
2 Customer Ratings
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4 Stars
100%
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1 Stars
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  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23332155
    Species: Rat
    Verified Customer | Posted 02/20/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23711726
    Species: Human
    Verified Customer | Posted 02/20/2015

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