Human/Mouse/Rat APE Antibody

Catalog #: AF1044
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1044
AF1044-SP

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Detection of Human, Mouse, and Rat APE by Western Blot.

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Product Details

Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat APE Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat APE in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human APE
Pro2-Leu318
Accession # P27695

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat APE by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Balb/3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat APE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1044) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for APE at approximately 40 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human APE by Simple Western^TM. Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for APE at approximately 45 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat APE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1044) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APE

Human APE (also known as Ref-1) is the apurinic/apyrimidinic (AP) endonuclease required for efficient DNA base excision repair (BER). Following the removal of a damaged base by a DNA glycosylase, APE cleaves the AP site to allow resynthesis and ligation to complete repair. In addition, APE/Ref-1 acts as a factor that regulates the redox state of multiple transcription factors, including c-Jun, c-Fos, NF-kappa B, and p53.

Long Name

Apurinic/Apyrimidinic Endonuclease

Entrez Gene IDs

328 (Human); 305605 (Rat)

Alternate Names

AP endonuclease 1; APE; APE1; APE-1; APEAPEX nuclease (multifunctional DNA repair enzyme); APENAP endonuclease class I; APEX deoxyribonuclease (apurinic or apyrimidinic); APEX nuclease (multifunctional DNA repair enzyme) 1; APEX nuclease; APEX1; Apurinic-apyrimidinic endonuclease 1; APXAP lyase; DNA-(apurinic or apyrimidinic site) lyase; EC 3.1; EC 4.2.99.18; HAP1apurinic/apyrimidinic (abasic) endonuclease; multifunctional DNA repair enzyme; protein REF-1; redox factor 1; Redox factor-1; REF1 apurinic/apyrimidinic exonuclease; REF-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Base Excision Repair

Citation for Human/Mouse/Rat APE Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Differential expression of APE1 and APE2 in germinal centers promotes error-prone repair and A:T mutations during somatic hypermutation.
Authors: Stavnezer J, Linehan E, Thompson M, Habboub G, Ucher A, Kadungure T, Tsuchimoto D, Nakabeppu Y, Schrader C
Proc Natl Acad Sci U S A, 2014;111(25):9217-22.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot