Human/Mouse/Rat beta -Actin Antibody

Catalog # Availability Size / Price Qty
MAB8929
MAB8929-SP
Detection of Human, Mouse, and Rat beta ‑Actin by Western Blot.
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Product Details
FAQs
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Reviews (5)

Human/Mouse/Rat beta -Actin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat  beta ‑Actin in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 937215
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Peptide containing a sequence at the N-terminus of human beta ‑Actin
Accession # P60709
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.01 µg/mL
See below
Simple Western
1 µg/mL
See below
Immunocytochemistry
0.1-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat beta ‑Actin by Western Blot. View Larger

Detection of Human, Mouse, and Rat
beta ‑Actin by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.01 µg/mL of Mouse Anti-Human/Mouse/Rat beta ‑Actin Monoclonal Antibody
(Catalog # MAB8929) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for beta ‑Actin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry beta ‑Actin  in NIH‑3T3 Mouse Cell Line. View Larger

beta ‑Actin in NIH‑3T3 Mouse Cell Line.
beta ‑Actin was detected in immersion fixed NIH‑3T3 mouse embryonic fibroblast cell line using Mouse Anti-Human/Mouse/Rat beta ‑Actin Monoclonal Antibody (Catalog # MAB8929) at 0.1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Cells were fixated in methanol. Specific staining was localized to cytoskeleton.

Simple Western Detection  of Human  beta ‑Actin by Simple WesternTM. View Larger

Detection of Human beta ‑Actin by Simple WesternTM. Simple Western lane view shows lysate of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for beta ‑Actin at approximately 49 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat
beta ‑Actin Monoclonal Antibody
(Catalog # MAB8929). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-Actin

Actin makes up the most abundant protein in many eukaryotic cell types. It polymerizes to form microfilaments that have an array of functions, including regulating contractility, motility, cytokinesis, phagocytosis, adhesion, cell morphology, and providing structural support. At least six types of Actin, falling into three categories, exist in higher eukaryotes. The three types, separated based on isoelectric point, include alpha ( alpha -Skeletal, alpha -Cardiac, alpha -Smooth Muscle), beta ( beta -Non-muscle) and gamma
( gamma -Smooth Muscle, gamma -non-muscle) isoforms.  Non-muscle beta - and gamma -actin are also known as cytoplasmic actin.

Entrez Gene IDs
60 (Human); 11461 (Mouse); 81822 (Rat)
Alternate Names
ACTB; actin, beta; actin, cytoplasmic 1; Beta Actin; beta cytoskeletal actin; betaActin; beta-Actin; BRWS1; PS1TP5-binding protein 1; PS1TP5BP1

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Cell and Tissue Staining Kits

Isotype Controls

Mouse IgG1 Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse/Rat beta -Actin Antibody

Average Rating: 4.6 (Based on 5 Reviews)

5 Star
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4 Star
40%
3 Star
0%
2 Star
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1 Star
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Human/Mouse/Rat beta -Actin Antibody
By Anonymous on 07/03/2018
Application: WB Sample Tested: Cancer cell lysates,Xenograft lysates Species: Human,Mouse

Human/Mouse/Rat beta -Actin Antibody
By Anonymous on 01/25/2018
Application: WB Sample Tested: IPS2 induced pluripotent stem cells Species: Mouse

Human/Mouse/Rat beta -Actin Antibody
By Anonymous on 12/15/2016
Application: ICC/IF Sample Tested: Endothelial progenitor cells Species: Human,Mouse

I have used for detecting expression of protein in Human and mouse Endothelial Progenitor cells (EPCs).


Human/Mouse/Rat beta -Actin Antibody
By Anonymous on 10/25/2016
Application: WB Sample Tested: Lineage depleted bone marrow cells Species: Mouse

Human/Mouse/Rat beta -Actin Antibody
By Anonymous on 08/22/2016
Application: WB Sample Tested: Liver tissue,Hepa 1-6 mouse hepatoma cell line,HepG2 human hepatocellular carcinoma cell line Species: Mouse,Human