Human/Mouse/Rat beta -Actin Antibody Summary
Accession # P60709
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human, Mouse, and Rat
beta ‑Actin by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.01 µg/mL of Mouse Anti-Human/Mouse/Rat beta ‑Actin Monoclonal Antibody
(Catalog # MAB8929) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for beta ‑Actin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
beta ‑Actin in NIH‑3T3 Mouse Cell Line.
beta ‑Actin was detected in immersion fixed NIH‑3T3 mouse embryonic fibroblast cell line using Mouse Anti-Human/Mouse/Rat beta ‑Actin Monoclonal Antibody (Catalog # MAB8929) at 0.1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Cells were fixated in methanol. Specific staining was localized to cytoskeleton.
Detection of Human beta ‑Actin by Simple WesternTM.
Simple Western lane view shows lysate of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for beta ‑Actin at approximately 49 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat
beta ‑Actin Monoclonal Antibody
(Catalog # MAB8929). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
up the most abundant protein in many eukaryotic cell types. It polymerizes to
form microfilaments that have an array of functions, including regulating
contractility, motility, cytokinesis, phagocytosis, adhesion, cell morphology,
and providing structural support. At least six types of Actin, falling into
three categories, exist in higher eukaryotes. The three types, separated based on
isoelectric point, include alpha ( alpha
-Skeletal, alpha -Cardiac, alpha -Smooth
Muscle), beta ( beta -Non-muscle) and gamma
( gamma -Smooth Muscle, gamma -non-muscle) isoforms. Non-muscle beta - and gamma -actin are also known as cytoplasmic actin.
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I have used for detecting expression of protein in Human and mouse Endothelial Progenitor cells (EPCs).