Detection of Human, Mouse, and Rat beta ‑Actin by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.01 µg/mL of Mouse Anti-Human/Mouse/Rat beta ‑Actin Monoclonal Antibody (Catalog # MAB8929) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for beta ‑Actin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
beta ‑Actin in NIH‑3T3 Mouse Cell Line.
beta ‑Actin was detected in immersion fixed NIH‑3T3 mouse embryonic fibroblast cell line using Mouse Anti-Human/Mouse/Rat beta ‑Actin Monoclonal Antibody (Catalog # MAB8929) at 0.1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Cells were fixated in methanol. Specific staining was localized to cytoskeleton.
Detection of Human beta ‑Actin by Simple WesternTM.
Simple Western lane view shows lysate of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for beta ‑Actin at approximately 49 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat beta ‑Actin Monoclonal Antibody (Catalog # MAB8929). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
up the most abundant protein in many eukaryotic cell types. It polymerizes to
form microfilaments that have an array of functions, including regulating
contractility, motility, cytokinesis, phagocytosis, adhesion, cell morphology,
and providing structural support. At least six types of Actin, falling into
three categories, exist in higher eukaryotes. The three types, separated based on
isoelectric point, include alpha ( alpha
-Skeletal, alpha -Cardiac, alpha -Smooth
Muscle), beta ( beta -Non-muscle) and gamma ( gamma -Smooth Muscle, gamma -non-muscle) isoforms. Non-muscle
beta - and gamma -actin are also known as cytoplasmic actin.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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We have 4 reviews tested in 3 species: Human,Mouse, Mouse, Mouse,Human.
We have 4 reviews tested in 2 applications: Western Blot, Immunocytochemistry/Immunofluorescence.