Human/Mouse/Rat beta -Actin Antibody

(6 citations)
(3 Reviews)
  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat  beta ‑Actin in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 937215
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Peptide containing a sequence at the N-terminus of human beta ‑Actin
    Accession # P60709
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.01 µg/mL
    See below
  • Simple Western
    1 µg/mL
    See below
  • Immunocytochemistry
    0.1-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat
beta ‑Actin by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.01 µg/mL of Mouse Anti-Human/Mouse/Rat beta ‑Actin Monoclonal Antibody
(Catalog # MAB8929) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for beta ‑Actin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
beta ‑Actin in NIH‑3T3 Mouse Cell Line.
beta ‑Actin was detected in immersion fixed NIH‑3T3 mouse embryonic fibroblast cell line using Mouse Anti-Human/Mouse/Rat beta ‑Actin Monoclonal Antibody (Catalog # MAB8929) at 0.1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Cells were fixated in methanol. Specific staining was localized to cytoskeleton.
Detection of Human beta ‑Actin by Simple WesternTM. Simple Western lane view shows lysate of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for beta ‑Actin at approximately 49 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat
beta ‑Actin Monoclonal Antibody
(Catalog # MAB8929). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-Actin
Actin makes up the most abundant protein in many eukaryotic cell types. It polymerizes to form microfilaments that have an array of functions, including regulating contractility, motility, cytokinesis, phagocytosis, adhesion, cell morphology, and providing structural support. At least six types of Actin, falling into three categories, exist in higher eukaryotes. The three types, separated based on isoelectric point, include alpha ( alpha -Skeletal, alpha -Cardiac, alpha -Smooth Muscle), beta ( beta -Non-muscle) and gamma
( gamma -Smooth Muscle, gamma -non-muscle) isoforms.  Non-muscle beta - and gamma -actin are also known as cytoplasmic actin.
  • Entrez Gene IDs:
    60 (Human); 11461 (Mouse); 81822 (Rat)
  • Alternate Names:
    ACTB; actin, beta; actin, cytoplasmic 1; Beta Actin; beta cytoskeletal actin; betaActin; beta-Actin; BRWS1; PS1TP5-binding protein 1; PS1TP5BP1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Cytoprotective Effects and Mechanisms of ?-17 Fatty Acid Desaturase in Injured Human Umbilical Vein Endothelial Cells (HUVECs)
    Authors: H Zhou, C Wang
    Med. Sci. Monit., 2017;23(0):1627-1635.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Pharmacological inhibition of macrophage toll-like receptor 4/nuclear factor-kappa B alleviates rhabdomyolysis-induced acute kidney injury
    Authors: RS Huang, JJ Zhou, YY Feng, M Shi, F Guo, SJ Gou, S Salerno, L Ma, P Fu
    Chin. Med. J., 2017;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. Hochu-ekki-to Treatment Improves Reproductive and Immune Modulation in the Stress-Induced Rat Model of Polycystic Ovarian Syndrome
    Authors: E Park, CW Choi, SJ Kim, YI Kim, S Sin, JP Chu, JY Heo
    Molecules, 2017;22(6):.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
  4. Microgravity Induction of TRAIL Expression in Preosteoclast Cells Enhances Osteoclast Differentiation
    Sci Rep, 2016;6(0):25143.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  5. Fatty acid-binding protein E-FABP restricts tumor growth by promoting IFN-beta responses in tumor-associated macrophages.
    Authors: Zhang Y, Sun Y, Rao E, Yan F, Li Q, Zhang Y, Silverstein K, Liu S, Sauter E, Cleary M, Li B
    Cancer Res, 2014;74(11):2986-98.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  6. Crosstalk between monocytes and renal mesangial cells via interaction of metalloproteinases and fractalkine in diabetic nephropathy.
    Authors: Wang Y, Wei Q, Liu Q, Li Z, Zhou L, Zou F, Yuan Y, Sun Z
    Mol Med Rep, 2013;8(6):1817-23.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

CTS002 14
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Isotype Controls
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Mouse IgG1 Isotype Control

Ctrl MAB002 138  
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Mouse IgG1 Isotype Control

Ctrl MAB002R 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Average Rating: 4.7 (Based on 3 reviews)

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Read what people are saying who have used Human/Mouse/Rat beta -Actin Antibody.

We have 1 review tested in 1 species: Mouse.
We have 3 reviews tested in 2 applications: Immunocytochemistry/Immunofluorescence, Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
  Excellent
 ICC/IF Anonymous 12/15/2016
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Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedEndothelial progenitor cells

Other Experimental Details

Other Experimental DetailsI have used for detecting expression of protein in Human and mouse Endothelial Progenitor cells (EPCs).
Western Blot Human/Mouse/Rat  beta -Actin Antibody MAB8929
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Excellent
 WB Mouse Anonymous 10/25/2016
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Image Details
Western Blot Human/Mouse/Rat  beta -Actin Antibody MAB8929
Western Blot: Human/Mouse/Rat beta -Actin Antibody [MAB8929]

Summary

ApplicationWestern Blot
Sample TestedLineage depleted bone marrow cells
SpeciesMouse
  Very Good
 WB Anonymous 08/22/2016
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Summary

ApplicationWestern Blot
Sample TestedLiver tissue,Hepa 1-6 mouse hepatoma cell line,HepG2 human hepatocellular carcinoma cell line

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