Human/Mouse/Rat beta -Actin Antibody

Catalog # Availability Size / Price Qty
MAB8929
MAB8929-SP

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Detection of Human, Mouse, and Rat  beta ‑Actin by Western Blot.
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Product Details
Citations (37)
FAQs
Supplemental Products
Reviews (10)

Human/Mouse/Rat beta -Actin Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat  beta ‑Actin in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 937215
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Peptide containing a sequence at the N-terminus of human beta ‑Actin
Accession # P60709
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.01 µg/mL
See below
Simple Western
1 µg/mL
See below
Immunocytochemistry
0.1-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat  beta -Actin antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat beta ‑Actin by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.01 µg/mL of Mouse Anti-Human/Mouse/Rat beta -Actin Monoclonal Antibody (Catalog # MAB8929) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for beta -Actin at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry beta -Actin antibody in NIH-3T3 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

beta ‑Actin in NIH‑3T3 Mouse Cell Line. beta -Actin was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Mouse Anti-Human/Mouse/Rat beta -Actin Monoclonal Antibody (Catalog # MAB8929) at 0.1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Cells were fixated in methanol. Specific staining was localized to cytoskeleton.

Simple Western Detection of Human  beta -Actin antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human beta ‑Actin by Simple WesternTM. Simple Western lane view shows lysate of A431 human epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for beta ‑Actin at approximately 49 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat beta ‑Actin Monoclonal Antibody (Catalog # MAB8929). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western View Larger

Detection of Human, Mouse, and Rat beta ‑Actin by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and C6 rat glioma cell line, loaded at 0.2 mg/mL. A specific band was detected for beta ‑Actin at approximately 48 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat beta ‑Actin Monoclonal Antibody (Catalog # MAB8929). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-Actin

Actin makes up the most abundant protein in many eukaryotic cell types. It polymerizes to form microfilaments that have an array of functions, including regulating contractility, motility, cytokinesis, phagocytosis, adhesion, cell morphology, and providing structural support. At least six types of Actin, falling into three categories, exist in higher eukaryotes. The three types, separated based on isoelectric point, include alpha ( alpha -Skeletal, alpha -Cardiac, alpha -Smooth Muscle), beta ( beta -Non-muscle) and gamma
( gamma -Smooth Muscle, gamma -non-muscle) isoforms.  Non-muscle beta - and gamma -actin are also known as cytoplasmic actin.

Entrez Gene IDs
60 (Human); 11461 (Mouse); 81822 (Rat)
Alternate Names
ACTB; actin, beta; actin, cytoplasmic 1; B-Actin; Beta Actin; beta cytoskeletal actin; betaActin; beta-Actin; BRWS1; I(2)-Actin; PS1TP5-binding protein 1; PS1TP5BP1

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Citations for Human/Mouse/Rat beta -Actin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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  1. Cinnamaldehyde and Curcumin Prime Akt2 for Insulin-Stimulated Activation
    Authors: Y Urasaki, TT Le
    Nutrients, 2022;14(16):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  2. Enhanced interpretation of 935 hotspot and non-hotspot RAS variants using evidence-based structural bioinformatics
    Authors: S Tripathi, NR Dsouza, AJ Mathison, E Leverence, R Urrutia, MT Zimmermann
    Computational and structural biotechnology journal, 2022;20(0):117-127.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. A VDAC1-mediated NEET protein chain transfers [2Fe-2S] clusters between the mitochondria and the cytosol and impacts mitochondrial dynamics
    Authors: O Karmi, HB Marjault, F Bai, S Roy, YS Sohn, M Darash Yah, F Morcos, K Ioannidis, Y Nahmias, PA Jennings, R Mittler, JN Onuchic, R Nechushtai
    Proceedings of the National Academy of Sciences of the United States of America, 2022;119(7):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Soy Phospholipids Exert a Renoprotective Effect by Inhibiting the Nuclear Factor Kappa B Pathway in Macrophages
    Authors: S Ohta, M Asanoma, N Irie, N Tachibana, M Kohno
    Metabolites, 2022;12(4):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. 2-Hydroxyglutarate destabilizes chromatin regulatory landscape and lineage fidelity to promote cellular heterogeneity
    Authors: M Kusi, M Zand, LL Lin, M Chen, A Lopez, CL Lin, CM Wang, ND Lucio, NB Kirma, J Ruan, TH Huang, K Mitsuya
    Cell Reports, 2022;38(2):110220.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Anagliptin prevents lipopolysaccharide (LPS)- induced inflammation and activation of macrophages
    Authors: F Yu, W Tian, J Dong
    International immunopharmacology, 2022;104(0):108514.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. KEAP1 Is Required for Artesunate Anticancer Activity in Non-Small-Cell Lung Cancer
    Authors: KS Hill, A McDowell, JR McCorkle, E Schuler, SR Ellingson, R Plattner, JM Kolesar
    Cancers, 2021;13(8):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Host and viral determinants for efficient SARS-CoV-2 infection of the human lung
    Authors: H Chu, B Hu, X Huang, Y Chai, D Zhou, Y Wang, H Shuai, D Yang, Y Hou, X Zhang, TT Yuen, JP Cai, AJ Zhang, J Zhou, S Yuan, KK To, IH Chan, KY Sit, DC Foo, IY Wong, AT Ng, TT Cheung, SY Law, WK Au, MA Brindley, Z Chen, KH Kok, JF Chan, KY Yuen
    Nature Communications, 2021;12(1):134.
    Species: Mesocricetus auratus (Hamster)
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Cyclo(Val-Pro) and Cyclo(Leu-Hydroxy-Pro) from Pseudomonas sp. (ABS-36) alleviates acute and chronic renal injury under in vitro and in vivo models (Ischemic reperfusion and unilateral ureter obstruction)
    Authors: K Hira, P Sharma, A Mahale, O Prakash Ku, A Sajeli Beg
    International immunopharmacology, 2021;103(0):108494.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Phosphorylation in the Charged Linker Modulates Interactions and Secretion of Hsp90beta
    Authors: L Weidenauer, M Quadroni
    Cells, 2021;10(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. Cytochrome P450 3A4 (CYP3A4) protein quantification using capillary western blot technology and total protein normalization
    Authors: JM Collins, D Wang
    Journal of pharmacological and toxicological methods, 2021;112(0):107117.
    Species: Human
    Sample Types: Tissue Lysates
    Applications: Simple Western
  12. Spaceflight and hind limb unloading induces an arthritic phenotype in knee articular cartilage and menisci of rodents
    Authors: AT Kwok, NS Mohamed, JF Plate, RR Yammani, S Rosas, TA Bateman, E Livingston, JE Moore, BA Kerr, J Lee, CM Furdui, L Tan, ML Bouxsein, VL Ferguson, LS Stodieck, DC Zawieja, MD Delp, XW Mao, JS Willey
    Scientific Reports, 2021;11(1):10469.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. Mesenchymal stromal cell mitochondrial transfer to human induced T-regulatory cells mediates FOXP3 stability
    Authors: JS Do, D Zwick, JD Kenyon, F Zhong, D Askew, AY Huang, W Van't Hof, M Finney, MJ Laughlin
    Scientific Reports, 2021;11(1):10676.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Cellular sensing of extracellular purine nucleosides triggers an innate IFN-&beta response
    Authors: R Dhanwani, M Takahashi, IT Mathews, C Lenzi, A Romanov, JD Watrous, B Pieters, CC Hedrick, CA Benedict, J Linden, R Nilsson, M Jain, S Sharma
    Sci Adv, 2020;6(30):eaba3688.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Menin and Menin-Associated Proteins Coregulate Cancer Energy Metabolism
    Authors: CW Chou, X Tan, CN Hung, B Lieberman, M Chen, M Kusi, K Mitsuya, CL Lin, M Morita, Z Liu, CL Chen, TH Huang
    Cancers (Basel), 2020;12(9):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  16. PAI-1-Dependent Inactivation of SMAD4-Modulated Junction and Adhesion Complex in Obese Endometrial Cancer
    Authors: LL Lin, ER Kost, CL Lin, P Valente, CM Wang, MG Kolonin, AC Daquinag, X Tan, N Lucio, CN Hung, CP Wang, NB Kirma, TH Huang
    Cell Rep, 2020;33(2):108253.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Secreted protein acidic and rich in cysteine (SPARC) knockout mice have greater outflow facility
    Authors: L Yu, Y Zheng, BJ Liu, MH Kang, JC Millar, DJ Rhee
    PLoS ONE, 2020;15(11):e0241294.
  18. Targeting QKI-7 in vivo restores endothelial cell function in diabetes
    Authors: C Yang, M Eleftheria, S Kelaini, T Morrison, MV González, R Caines, N Edwards, A Yacoub, K Edgar, A Moez, A Ivetic, A Zampetaki, L Zeng, FL Wilkinson, N Lois, AW Stitt, DJ Grieve, A Margariti
    Nat Commun, 2020;11(1):3812.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Targeting QKI-7 in vivo restores endothelial cell function in diabetes
    Authors: C Yang, M Eleftheria, S Kelaini, T Morrison, MV González, R Caines, N Edwards, A Yacoub, K Edgar, A Moez, A Ivetic, A Zampetaki, L Zeng, FL Wilkinson, N Lois, AW Stitt, DJ Grieve, A Margariti
    Nat Commun, 2020;11(1):3812.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  20. The critical role of MMP14 in adipose tissue remodeling during obesity
    Authors: X Li, Y Zhao, C Chen, L Yang, HH Lee, Z Wang, N Zhang, MG Kolonin, Z An, X Ge, PE Scherer, K Sun
    Mol. Cell. Biol., 2020;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Simiao Decoction Alleviates Gouty Arthritis by Modulating Proinflammatory Cytokines and the Gut Ecosystem
    Authors: X Lin, T Shao, L Huang, X Wen, M Wang, C Wen, Z He
    Front Pharmacol, 2020;11(0):955.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  22. Activity of fibroblast-like synoviocytes in rheumatoid arthritis was impaired by dickkopf-1 targeting siRNA
    Authors: YY Liu, SY Wang, YN Li, WJ Bian, LQ Zhang, YH Li, L Long, X Liu, XW Zhang, ZG Li
    Chin. Med. J., 2020;0(6):679-686.
    Species: Human
    Sample Types: Cell Lysate
    Applications: Western Blot
  23. Selenium Nanoparticles Pre-Treatment Reverse Behavioral, Oxidative Damage, Neuronal Loss and Neurochemical Alterations in Pentylenetetrazole-Induced Epileptic Seizures in Mice
    Authors: X Yuan, Z Fu, P Ji, L Guo, AO Al-Ghamdy, A Alkandiri, OA Habotta, AE Abdel Mone, RB Kassab
    Int J Nanomedicine, 2020;15(0):6339-6353.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  24. Milk-derived exosomes (MDEs) have a different biological effect on�normal fetal colon epithelial cells compared to colon tumor cells in a miRNA-dependent manner
    Authors: S Reif, Y Elbaum Shi, R Golan-Gers
    J Transl Med, 2019;17(1):325.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Royal jelly attenuates cadmium-induced nephrotoxicity in male mice
    Authors: RS Almeer, GI AlBasher, S Alarifi, S Alkahtani, D Ali, AE Abdel Mone
    Sci Rep, 2019;9(1):5825.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  26. B-cell lymphoma 2 is associated with advanced tumor grade and clinical stage, and reduced overall survival in young Chinese patients with colorectal carcinoma
    Authors: J Wang, G He, Q Yang, L Bai, B Jian, Q Li, Z Li
    Oncol Lett, 2018;15(6):9009-9016.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  27. Memantine protects against ischemia/reperfusion-induced brain endothelial permeability
    Authors: Y Liu, Y Huang, Y Xu, P Qu, M Wang
    IUBMB Life, 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. A homozygous loss-of-function mutation leading to CYBC1 deficiency causes chronic granulomatous disease
    Authors: GA Arnadottir, GL Norddahl, S Gudmundsdo, AB Agustsdott, S Sigurdsson, BO Jensson, K Bjarnadott, F Theodors, S Benonisdot, EV Ivarsdotti, A Oddsson, RP Kristjanss, G Sulem, KF Alexanders, T Juliusdott, KR Gudmundsso, J Saemundsdo, A Jonasdotti, A Jonasdotti, A Sigurdsson, P Manzanillo, SA Gudjonsson, GA Thorisson, OT Magnusson, G Masson, KB Orvar, H Holm, S Bjornsson, R Arngrimsso, DF Gudbjartss, U Thorsteins, I Jonsdottir, A Haraldsson, P Sulem, K Stefansson
    Nat Commun, 2018;9(1):4447.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  29. Association between circadian gene CLOCK and cisplatin resistance in ovarian cancer cells: A preliminary study
    Authors: H Xu, Z Wang, G Mo, H Chen
    Oncol Lett, 2018;15(6):8945-8950.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  30. DNA methylation affects metastasis of renal cancer and is associated with TGF-?/RUNX3 inhibition
    Authors: J Zheng, Y Mei, P Xiang, G Zhai, N Zhao, C Xu, M Liu, Z Pan, K Tang, D Jia
    Cancer Cell Int., 2018;18(0):56.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  31. Pharmacological inhibition of macrophage toll-like receptor 4/nuclear factor-kappa B alleviates rhabdomyolysis-induced acute kidney injury
    Authors: RS Huang, JJ Zhou, YY Feng, M Shi, F Guo, SJ Gou, S Salerno, L Ma, P Fu
    Chin. Med. J., 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  32. Hochu-ekki-to Treatment Improves Reproductive and Immune Modulation in the Stress-Induced Rat Model of Polycystic Ovarian Syndrome
    Authors: E Park, CW Choi, SJ Kim, YI Kim, S Sin, JP Chu, JY Heo
    Molecules, 2017;22(6):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  33. Cytoprotective Effects and Mechanisms of ?-17 Fatty Acid Desaturase in Injured Human Umbilical Vein Endothelial Cells (HUVECs)
    Authors: H Zhou, C Wang
    Med. Sci. Monit., 2017;23(0):1627-1635.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  34. Anti-neoplastic drugs increase caveolin-1-dependent migration, invasion and metastasis of cancer cells
    Authors: NI Díaz-Valdi, CC Calderón, JE Díaz, L Lobos-Gonz, H Sepulveda, RJ Ortíz, S Martinez, V Silva, HJ Maldonado, P Silva, S Wehinger, VA Burzio, VA Torres, M Montecino, L Leyton, AFG Quest
    Oncotarget, 2017;8(67):111943-111965.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  35. Microgravity Induction of TRAIL Expression in Preosteoclast Cells Enhances Osteoclast Differentiation
    Sci Rep, 2016;6(0):25143.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  36. Fatty acid-binding protein E-FABP restricts tumor growth by promoting IFN-beta responses in tumor-associated macrophages.
    Authors: Zhang Y, Sun Y, Rao E, Yan F, Li Q, Zhang Y, Silverstein K, Liu S, Sauter E, Cleary M, Li B
    Cancer Res, 2014;74(11):2986-98.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  37. Crosstalk between monocytes and renal mesangial cells via interaction of metalloproteinases and fractalkine in diabetic nephropathy.
    Authors: Wang Y, Wei Q, Liu Q, Li Z, Zhou L, Zou F, Yuan Y, Sun Z
    Mol Med Rep, 2013;8(6):1817-23.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human/Mouse/Rat beta -Actin Antibody

Average Rating: 4.8 (Based on 10 Reviews)

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Human/Mouse/Rat beta -Actin Antibody
By Anonymous on 08/09/2021
Application: WB Sample Tested: Skin tissue Species: Mouse

Human/Mouse/Rat beta-Actin Antibody
By Anonymous on 03/07/2020
Application: WB Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human

Human/Mouse/Rat beta-Actin Antibody
By Anonymous on 08/12/2019
Application: WB Sample Tested: A549 human lung carcinoma cell line Species: Human

Used at 50 ng/ml


Human/Mouse/Rat beta-Actin Antibody
By Joseph Moore on 08/06/2019
Application: WB Sample Tested: Cartilage tissue Species: Human

Antibody dilution was 1:100. Beta-Actin clearly expressed in mouse meniscus lysates, detected with ECL kit.


Human/Mouse/Rat beta-Actin Antibody
By Agnès Tessier on 06/17/2019
Application: Simple Western Sample Tested: Skin tissue Species: Human

Actin expression in reconstructed human epidermis lysate (200 µg/mL protein concentration) detected in chemiluminescence.
Antibody dilution 1:50, works also at 1:100 dilution.


Human/Mouse/Rat beta -Actin Antibody
By Anonymous on 07/03/2018
Application: WB Sample Tested: Cancer cell lysates,Xenograft lysates Species: Human and Mouse

Human/Mouse/Rat beta -Actin Antibody
By Anonymous on 01/25/2018
Application: WB Sample Tested: IPS2 induced pluripotent stem cells Species: Mouse

Human/Mouse/Rat beta -Actin Antibody
By Anonymous on 12/15/2016
Application: ICC/IF Sample Tested: Endothelial progenitor cells Species: Human and Mouse

I have used for detecting expression of protein in Human and mouse Endothelial Progenitor cells (EPCs).


Human/Mouse/Rat beta -Actin Antibody
By Anonymous on 10/25/2016
Application: WB Sample Tested: Lineage depleted bone marrow cells Species: Mouse

Human/Mouse/Rat beta -Actin Antibody
By Anonymous on 08/22/2016
Application: WB Sample Tested: Hepa 1-6 mouse hepatoma cell line,HepG2 human hepatocellular carcinoma cell line,Liver tissue Species: Human and Mouse