Human/Mouse/Rat Calcineurin B Antibody

Catalog # Availability Size / Price Qty
Detection of Human/Mouse/Rat Calcineurin B by Western Blot.
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Human/Mouse/Rat Calcineurin B Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects endogenous human, mouse, and rat Calcineurin B.
Monoclonal Mouse IgG2A Clone # 212306
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Calcineurin B
Accession # P63098
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse/Rat Calcineurin B antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat Calcineurin B by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, TS1 mouse helper T cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Calcineurin B Monoclonal Antibody (Catalog # MAB1348) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Calcineurin B at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Calcineurin B


Calcineurin, also called Protein Phosphatase 2B,

PP2B, PPP2B, Protein Phosphatase 3, and PPP3, is

an enzyme that dephosphorylates serine and

threonine residues in proteins. It is a heterodimer of

a 59,000 dalton catalytic A subunit and a

1,900 dalton regulatory B subunit that is activated

by the binding of calcium ions and calmodulin (1).

Calcineurin is expressed in many tissues, but its

levels are highest in the brain, where it may play a

role in learning and memory (2). It has many

substrates, including NFAT, a transcription factor

that is activated by dephosphorylation (3).

Complexes of the immunosuppressants cyclosporin

and FK506 with immunophilin proteins such as

cyclophilin and FKBP12 are potent and specific

inhibitors of Calcineurin activity (4). Alterations in

Calcineurin activity are suspected to play a role in

cardiac hypertrophy (5) and graft versus host

disease in organ transplantation (6).


1. Stemmer, P.M and C.B. Klee (1994) Biochemistry 33:6859.

2. Zeng H. et al. (2001) Cell 107:617.

3. Okamura, H. et al. (2000) Molecular Cell 6:539.

4. Liu, J. et al. (1992) Biochemistry 31:3896.

5. Molkentin, J.D. (2000) Circulation Research 87:731.

6. Sanquer, S.A. et al. (2004) Transplantation 77:854.

Entrez Gene IDs
5534 (Human); 19058 (Mouse); 29748 (Rat)
Alternate Names
Calcineurin B; calcineurin subunit B type 1; CALNB1; CNA2; Cnb1; MCIP1; PP2B beta 1; PPP3R1; Protein phosphatase 2B regulatory subunit 1; protein phosphatase 2B regulatory subunit B alpha; protein phosphatase 3 (formerly 2B), regulatory subunit B (19kD), alpha isoform(calcineurin B, type I); protein phosphatase 3 (formerly 2B), regulatory subunit B, 19kDa, alpha isoform(calcineurin B, type I); protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform; Protein phosphatase 3 regulatory subunit B alpha isoform 1; protein phosphatase 3, regulatory subunit B, alpha; type I (19kDa)

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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Human/Mouse/Rat Calcineurin B Antibody
By Anonymous on 10/26/2021
Application: WB Sample Tested: SK-Mel-28 human malignant melanoma cell line Species: Human