Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Complexin-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Complexin-2
Met1-Lys134
Accession # Q6PUV4

Specificity

Detects endogenous human, mouse and rat Complexin-2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Complexin‑2 Antibody

Detection of Human/Mouse/Rat Complexin-2 antibody by Western Blot.

Detection of Human/Mouse/Rat Complexin‑2 by Western Blot.

Western blot shows lysates of mouse, human, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Complexin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5085) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Complexin-2 at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Complexin-2 antibody in SH-SY5Y Human Neuroblastoma Cells by Immunocytochemistry (ICC).

Complexin‑2 in SH‑SY5Y Human Neuroblastoma Cells.

SH-SY5Y human neuro-blastoma cells were cultured overnight in the presence of 1 mM Retinoic Acid (Catalog # 0695/50) prior to immersion fixation. Complexin-2 was detected using a Goat Anti-Human/Mouse/Rat Complexin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5085). The cells were stained with the NorthernLights 557-conjugated Donkey Anti-Goat IgG Affinity-purified Secondary Antibody (red; Catalog # NL001). Actin filaments were stained with FITC-conjugated Phalloidin (green) and the nuclei were counterstained with DAPI (blue). Complexin-2 immunoreactivity was localized to synaptic vesicles. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Complexin‑2 in L6 rat myoblast cell line and A549 human lung carcinoma cell line.

Complexin‑2 was detected in immersion fixed L6 rat myoblast cell line and A549 human lung carcinoma cell line using Goat Anti-Human/Mouse/Rat Complexin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5085) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Applications for Human/Mouse/Rat Complexin‑2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed SH-SY5Y human neuroblastoma cell line

Western Blot

1 µg/mL
Sample: Mouse, human, and rat brain tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complexin-2

Complexin-2 (CPX II; also known as synaphin-1) is a 19 kDa, cytosolic protein that belongs to the complexin/synaphin family of molecules. It is expressed in excitatory neurons and serves as a block against premature granule release by its binding to the cis-SNARE complex that contains SNAP25, VAMP2 and STX1A. Human Complexin-2 is 134 amino acids (aa) in length and contains one coiled-coil domain (aa 28‑84) that incorporates part of a SNARE complex interaction sequence (aa 41‑97). There is one potential splice variant that shows a premature truncation after Glu114. Human Complexin-2 is identical in aa sequence to that for mouse and rat Complexin-2.

Alternate Names

CPLX2, CPX-II, CPX2, Hfb1, Synaphin 1

Entrez Gene IDs

10814 (Human); 12890 (Mouse); 116657 (Rat)

Gene Symbol

CPLX2

UniProt

Q6PUV4 (Human)

Additional Complexin-2 Products

Product Documents for Human/Mouse/Rat Complexin‑2 Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat Complexin‑2 Antibody

For research use only

Citations for Human/Mouse/Rat Complexin‑2 Antibody

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Protocols

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