Human/Mouse/Rat DGCR2 Antibody

Catalog # Availability Size / Price Qty
MAB10443-100
MAB10443-SP
Detection of Human, Mouse, and Rat DGCR2 by Western Blot.
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Human/Mouse/Rat DGCR2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat DGCR2 in Western blots. Detects human DGCR2 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 1019908
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese Hamster Ovary cell line CHO-derived human DGCR2
Glu22-Ala349
Accession # P98153
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat DGCR2 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat DGCR2 by Western Blot. Western blot shows lysates of Hep3B human hepatocellular carcinoma cell line, Hepa 1-6 mouse hepatoma cell line, and H4-II-E-C3 rat hepatoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat DGCR2 Monoclonal Antibody (Catalog # MAB10443) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for DGCR2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DGCR2

DiGeorge Syndrome Critical Region 2 (DGCR2), also known as IDD, DGS-C, LAN, and SEZ-12, is an adhesion receptor protein located on the long arm of chromosome 22 (1). The DGCR2 gene encodes an integral membrane protein, consisting of an extracellular domain, a single transmembrane region and a cytoplasmic tail (1). The mature extracellular domain (ECD) of DGCR2 contains both a C-type lectin domain and a cysteine-rich region similar to that of the low density lipoprotein receptor (LDLR) (1, 3). The mature ECD of human DGCR2 shares 93% and 92% amino acid sequence identity with mouse and rat, respectively. DGCR2 is expressed during neurodevelopment in human brain tissues (3). Deletion of the 22q11.2 region results in an extremely variable disorder called 22q11.2 deletion syndrome, with a phenotype ranging from very mild symptoms to severe intellectual disability, facial dysmorphism, heart defects, and urogenital abnormalities (4). Recent studies suggest that DGCR2 regulates critical steps of early cortico-genesis possibly through a Reelin-dependent mechanism. Deletion of DGCR2 has a pathogenic impact on cortical formation by reducing protein expression level, and it plays a critical role in vulnerability to schizophrenia (5). Furthermore, expression of DGCR2 together with USP18 gene may serve as a progonostic marker for muscle invasive bladder cancer survival in patients (5).

References
  1. Augusseau, S. et al. (1986) Hum. Genet. 74:206.
  2. Mugikura, S. et al. (2016) Biochem Biophys Rep. 5:120.
  3. Kajiwara, K. et al. (1996) Biochem. Biophyis. Res. Commun. 222:144.
  4. Vaisvilas, M. et al. (2018) Balkan J Med Genet. 21:87.
  5. Molinard-Chenu A. and Dayer A. (2017) Biol. Psych. 83:692.
Long Name
DiGeorge Syndrome Critical Region Gene 2
Entrez Gene IDs
9993 (Human); 13356 (Mouse); 360742 (Rat)
Alternate Names
DGCR2; DGS-C; DiGeorge syndrome critical region gene 2; IDDDKFZp686I1730; integral membrane protein deleted in DiGeorge syndrome; integral membrane protein DGCR2/IDD; KIAA0163; KIAA0163DiGeorge syndrome critical region protein 2; LAN; SEZ-12

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