Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 613531
View all DGK-gamma Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DGK‑ gamma
Gly2-Ser126
Accession # P49619

Specificity

Detects human, mouse, and rat DGK‑ gamma in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat DGK‑ gamma Antibody

Detection of Human, Mouse, and Rat DGK-? antibody by Western Blot.

Detection of Human, Mouse, and Rat DGK‑ gamma by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MDA-MB-468 human breast cancer cell line, C2C12 mouse myoblast cell line, C6 rat glioma cell line, and SH-SY5Y human neuroblastoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat DGK- gamma Monoclonal Antibody (Catalog # MAB6389) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK- gamma at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat DGK‑ gamma Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MDA‑MB‑468 human breast cancer cell line, C2C12 mouse myoblast cell line, C6 rat glioma cell line, and SH‑SY5Y human neuroblastoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: DGK-gamma

DGK-gamma (Diacylglycerol kinase gamma) is a 90 kDa, 791 amino acid (aa), type I member of the eukaryocytic diacylglycerol kinase family of enzymes. DGK-gamma possesses EF-hand, C1/Cys-rich zinc finger and catalytic domains, and is highly enriched in the retina and in cerebellar Purkinje cells. Expression elsewhere is limited to inactive isoforms missing kinase domain aa 451‑475, or zinc finger aa 333‑372. Upon calcium and PKC gamma -mediated activation, it translocates to the plasma membrane. DGK-gamma is also found in the nucleus, where it may promote cell cycle progression. Over aa 1‑126, human DGK-gamma shares 83% and 79% aa identity with mouse and rat DGK-gamma, respectively.

Long Name

Diacylglycerol Kinase, gamma 90kDa

Alternate Names

DAGK3, DGKG, DGKgamma

Entrez Gene IDs

1608 (Human); 110197 (Mouse); 25666 (Rat)

Gene Symbol

DGKG

UniProt

P49619 (Human)

Additional DGK-gamma Products

Product Documents for Human/Mouse/Rat DGK‑ gamma Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat DGK‑ gamma Antibody

For research use only

Related Research Areas

Intracellular Kinases

Customer Reviews for Human/Mouse/Rat DGK‑ gamma Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat DGK‑ gamma Antibody and earn rewards!

Have you used Human/Mouse/Rat DGK‑ gamma Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies