Human/Mouse/Rat DGK-gamma Antibody

Catalog # Availability Size / Price Qty
Detection of Human, Mouse, and Rat DGK‑ gamma  by Western Blot.
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Product Details
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Human/Mouse/Rat DGK-gamma Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse, and rat DGK‑ gamma in Western blots.
Monoclonal Mouse IgG2B Clone # 613531
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human DGK‑ gamma
Accession # P49619
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat DGK-? antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat DGK‑ gamma by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MDA-MB-468 human breast cancer cell line, C2C12 mouse myoblast cell line, C6 rat glioma cell line, and SH-SY5Y human neuroblastoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat DGK- gamma Monoclonal Antibody (Catalog # MAB6389) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK- gamma at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DGK-gamma

DGK-gamma (Diacylglycerol kinase gamma) is a 90 kDa, 791 amino acid (aa), type I member of the eukaryocytic diacylglycerol kinase family of enzymes. DGK-gamma possesses EF-hand, C1/Cys-rich zinc finger and catalytic domains, and is highly enriched in the retina and in cerebellar Purkinje cells. Expression elsewhere is limited to inactive isoforms missing kinase domain aa 451‑475, or zinc finger aa 333‑372. Upon calcium and PKC gamma -mediated activation, it translocates to the plasma membrane. DGK-gamma is also found in the nucleus, where it may promote cell cycle progression. Over aa 1‑126, human DGK-gamma shares 83% and 79% aa identity with mouse and rat DGK-gamma, respectively.

Long Name
Diacylglycerol Kinase, gamma 90kDa
Entrez Gene IDs
1608 (Human); 110197 (Mouse); 25666 (Rat)
Alternate Names
DAG kinase gamma; DAGK3; DAGK3MGC104993; DGKG; DGKgamma; DGK-gamma; diacylglycerol kinase gamma; diacylglycerol kinase, gamma (90kD); diacylglycerol kinase, gamma 90kDa; diacylglyerol kinase gamma; Diglyceride kinase gamma; EC; MGC133330

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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