Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 239727
View all EG-VEGF/PK1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat EG-VEGF
Ala20-Phe105 (predicted)
Accession # Q8R414

Specificity

Detects human, mouse, and rat EG-VEGF in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human EG-VEGF and recombinant mouse EG-VEGF is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human/Mouse/Rat EG‑VEGF/PK1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample:

Recombinant Human EG-VEGF/PK1 (Catalog # 1209-EV)
Recombinant Mouse EG-VEGF/PK1 (Catalog # 2464-EV)
Recombinant Rat EG-VEGF/PK1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EG-VEGF/PK1

EG-VEGF, a member of the prokineticin family of secreted proteins, is expressed in multiple tissues including the gastrointestinal tract, testis, ovary, placenta and adrenal glands. It binds to the G protein-coupled receptors, EG-VEGF/PK1-R1 and EG-VEGF/PK2-R2, leading to stimulation of smooth muscle contraction and tissue‑specific angiogenesis.

Long Name

Endocrine Gland-derived Vascular Endothelial Growth Factor

Alternate Names

EGVEGF, PROK1, Prokineticin 1

Entrez Gene IDs

84432 (Human); 246691 (Mouse); 192205 (Rat)

Gene Symbol

PROK1

UniProt

Q8R414

Additional EG-VEGF/PK1 Products

Product Documents for Human/Mouse/Rat EG‑VEGF/PK1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat EG‑VEGF/PK1 Antibody

For research use only

Citations for Human/Mouse/Rat EG‑VEGF/PK1 Antibody

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