Human/Mouse/Rat FABP1/L-FABP Antibody

Catalog # Availability Size / Price Qty
AF2964
AF2964-SP
Detection of Human FABP1/L‑FABP by Western Blot.
5 Images
Product Details
FAQs
Supplemental Products
Reviews (4)

Human/Mouse/Rat FABP1/L-FABP Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human FABP1/L‑FABP in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant human FABP2, 3, 4, 5, 7, and recombinant mouse FABP9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FABP1/L‑FABP
Met1-Ile127
Accession # P07148
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
5-10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human FABP1/L-FABP antibody by Western Blot. View Larger

Detection of Human FABP1/L‑FABP by Western Blot. Western blot shows lysates of human liver tissue, human colon tissue, and human kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/ Mouse/Rat FABP1/L-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2964) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP1/L-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human, Mouse, and Rat FABP1/L-FABP antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat FABP1/L‑FABP by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse/Rat FABP1/L-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2964) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP1/L-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry FABP1 antibody in Human Liver by Immunohistochemistry (IHC-P). View Larger

FABP1 in Human Liver. FABP1 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human/Mouse/Rat FABP1/L-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2964) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human FABP1/L-FABP antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human FABP1/L‑FABP by Simple WesternTM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP1/L‑FABP at approximately 14 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat FABP1/L‑FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2964). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western Detection of Human and Mouse FABP1/L-FABP antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human and Mouse FABP1/L‑FABP by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP1/L-FABP at approximately 14-16 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat FABP1/L-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2964) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP1/L-FABP

FABP1, also known as liver FABP (L-FABP, Z-protein. and squalene and sterol-carrier protein [SCP]) is a member of the intracellular FABP family. It is highly expressed in the liver, intestine, kidney, and lung. FABP1 binds free fatty acids, their co-enzyme A derivatives, and may be involved in intracellular lipid transport.

Long Name
Fatty Acid-Binding Protein 1
Entrez Gene IDs
2168 (Human); 14080 (Mouse); 24360 (Rat)
Alternate Names
FABP1; FABPL; fatty acid binding protein 1, liver; fatty acid-binding protein, liver; LFABP; L-FABP; L-FABPFatty acid-binding protein 1; Liver-type fatty acid-binding protein

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse/Rat FABP1/L-FABP Antibody

Average Rating: 3.8 (Based on 4 Reviews)

5 Star
0%
4 Star
75%
3 Star
25%
2 Star
0%
1 Star
0%

Have you used Human/Mouse/Rat FABP1/L-FABP Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human/Mouse/Rat FABP1/L-FABP Antibody
By Anonymous on 06/10/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse/Rat FABP1/L-FABP Antibody
By Anonymous on 11/11/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human

Human/Mouse/Rat FABP1/L-FABP Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse/Rat FABP1/L-FABP Antibody
By Anonymous on 11/02/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human