Detects human Fetuin B in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) Fetuin A, rhCystatin A, rhCystatin B, rhCystatin C, rhCystatin D, rhCystatin E/M, rhCystatin S, rhCystatin SA, and rhCystatin SN is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human Fetuin B Met19-Pro382 Accession # Q9UGM5
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human, Mouse, and Rat Fetuin B by Western Blot. Western blot shows human serum, human placenta tissue lysate, mouse serum, and rat placenta tissue lysate. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human/Mouse/Rat Fetuin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1725) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Fetuin B at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Fetuin B by Simple WesternTM. Simple Western lane view shows lysates of human placenta tissue and rat placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Fetuin B at approximately 64 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human/Mouse/Rat Fetuin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1725) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fetuin B
Fetuins are members of the cystatin superfamily of cysteine protease inhibitors (1‑3). Additional members of this superfamily are kininogen and histidine-rich glycoprotein. Fetuin A and B are two known members of the fetuin family. Hepatocytes are believed to be the principal cellular source, but other cell types also express it (4, 5). Fetuin A, also known as alpha 2-Heremans-Schmid glycoprotein, is an inhibitor of basic calcium phosphate precipitation and a negative acute-phase protein (6, 7). Normal circulating levels of Fetuin A in adults (300‑600 ug/mL) fall significantly (30‑50%) during injury and infection (7). Fetuin B is a newer member whose function is not fully characterized (1, 2). Fetuin A and B display similarities and differences in their characteristics. Fetuin B exhibits reduction of calcification, while both mRNA levels were down‑regulated during the acute phase in inflammation-induced rats (4). However, they share only 20% amino acid sequence identity (2). The amounts of Fetuin B in human serum, unlike Fetuin A, vary with gender and are higher in females than in males (4).
Oliver, E. et al. (1999) Genomics. 57:352.
Oliver, E. et al. (2000)Biochem. J.350:589.
Kellemann, J. et al. 1989, J. Biol. Chem. 264:14121.
Denecke, B. et al. (2003) Biochem. J. 376:135.
Schäfer, C. et al. (2003) J. Clin. Invest. 112:357.
Dziegielewska, K. M. et al. (1996) Histochem.Cell Biol.106:319.
Gangneux, C. et al. (2003) Nucleic Acids Res. 31:5957.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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