Human/Mouse/Rat FKBP38 Antibody

(1 citations)
(1 Review)
  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat FKBP38 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human FKBP-12, -12.6, -13, -25, -51, or -52 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli derived recombinant mouse FKBP38
    Met1-Gly326
    Accession # AAH03739
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry

FKBP38 in Human Intestine. FKBP38 was detected in immersion fixed paraffin-embedded sections of human intestine using 15 µg/mL Goat Anti-Human/Mouse/Rat FKBP38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3580) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the nucleus of epithelial cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. This application has not been tested in rat or mouse tissue.

Detection of Human/Mouse/Rat FKBP38 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, MDA‑MB‑468 human breast cancer cell line, A549 human lung carcinoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat FKBP38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3580) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for FKBP38 at approximately 60 - 64 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Human FKBP38 by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and MDA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for FKBP38 at approximately 58 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat FKBP38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3580) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FKBP38

FK506 Binding Proteins (FKBPs) are intracellular receptors for the immuno-suppressive drug FK506. The FKBP/FK506 complex exerts its immunosuppressive effects by inhibiting calcineurin, a calcium- and calmodulin-dependent serine/threonine phosphatase that functions as a critical signaling molecule during T cell activation. FKBP38, also known as FKBP8, is a 355 amino acid (aa) protein with a calculated molecular weight of 38.7 kDa and an apparent molecular mass of ~60-64 kDa in SDS-PAGE. FKBP38 binds to and inhibits calcineurin even in the absence of FK506, indicating that FKBP38 is a constitutively active inhibitor of calcineurin. Additionally, FKBP38 immunoprecipitates with Bcl-2 and Bcl-xL, suggesting that FKBP38 may regulate apoptosis by anchoring Bcl-2 and Bcl-xL to mitochondrial membranes. Mouse FKBP38 shares 94% and 87% sequence identity with human and rat FKBP38, respectively.

  • Long Name:
    38 kDa FK506 Binding Protein
  • Entrez Gene IDs:
    23770 (Human); 14232 (Mouse); 290652 (Rat)
  • Alternate Names:
    38 kDa FK506-binding protein; EC 5.2.1.8,38 kDa FKBP; FK506 binding protein 8, 38kDa; FK506-binding protein 8 (38kD); FK506-binding protein 8; FKBP38; FKBP-38; FKBP38PPIase FKBP8; FKBP8; FKBP-8; FKBPr38; hFKBP38; peptidyl-prolyl cis-trans isomerase FKBP8; rotamase
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Hepatitis C virus NS5A activates the mammalian target of rapamycin (mTOR) pathway, contributing to cell survival by disrupting the interaction between FK506-binding protein 38 (FKBP38) and mTOR.
    Authors: Peng L, Liang D, Tong W
    J. Biol. Chem., 2010;285(27):20870-81.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

CTS013 4
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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human/Mouse/Rat FKBP38 Antibody AF3580
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Very Good
 WB Human Anonymous 08/17/2016
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Western Blot Human/Mouse/Rat FKBP38 Antibody AF3580
Western Blot: Human/Mouse/Rat FKBP38 Antibody [AF3580]

Summary

ApplicationWestern Blot
Sample TestedHEK293 human embryonic kidney cell line
SpeciesHuman

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