Human/Mouse/Rat Frk Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat Frk in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Frk
    Met1-Glu107
    Accession # P42685
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed paraffin-embedded sections of human breast cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Frk by Western Blot.

Western blot shows lysates of MCF‑7 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, and NTera‑2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse/Rat Frk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3766) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Frk at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Frk

Fyn-related kinase (Frk) is a tyrosine kinase of the Src family. Also known as Rak, Gtk, and Iyk, Frk is predominantly expressed in epithelial tissues. Frk protein is 49% and 47% identical to Fyn and Csk, respectively. Src family kinases regulate an array of cellular processes, including growth factor signaling, cytoskeleton dynamics, and cell proliferation.

  • Long Name:
    FYN-related Kinase
  • Entrez Gene IDs:
    2444 (Human); 14302 (Mouse); 79209 (Rat)
  • Alternate Names:
    EC 2.7.10; Frk; fyn-related kinaseProtein-tyrosine kinase 5; Gtk; GTKEC 2.7.10.2; Nuclear tyrosine protein kinase RAK; PTK5 protein tyrosine kinase 5; PTK5; Rak; RAKPTK5; tyrosine-protein kinase FRK
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