Human/Mouse/Rat Frk Antibody

Catalog # Availability Size / Price Qty
AF3766
AF3766-SP
Detection of Human Frk by Western Blot.
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Human/Mouse/Rat Frk Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat Frk in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Frk
Met1-Glu107
Accession # P42685
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human breast cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Frk antibody by Western Blot. View Larger

Detection of Human Frk by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, and NTera-2 human testicular embryonic carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse/Rat Frk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3766) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Frk at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frk

Fyn-related kinase (Frk) is a tyrosine kinase of the Src family. Also known as Rak, Gtk, and Iyk, Frk is predominantly expressed in epithelial tissues. Frk protein is 49% and 47% identical to Fyn and Csk, respectively. Src family kinases regulate an array of cellular processes, including growth factor signaling, cytoskeleton dynamics, and cell proliferation.

Long Name
FYN-related Kinase
Entrez Gene IDs
2444 (Human); 14302 (Mouse); 79209 (Rat)
Alternate Names
EC 2.7.10; Frk; fyn-related kinaseProtein-tyrosine kinase 5; Gtk; GTKEC 2.7.10.2; Nuclear tyrosine protein kinase RAK; PTK5 protein tyrosine kinase 5; PTK5; Rak; RAKPTK5; tyrosine-protein kinase FRK

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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