Human/Mouse/Rat GRK5 Antibody

Catalog # Availability Size / Price Qty
AF4539
AF4539-SP
Detection of Human/Mouse/Rat GRK5 by Western Blot.
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Product Details
Citations (3)
FAQs
Supplemental Products
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Human/Mouse/Rat GRK5 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects endogenous human, mouse, and rat GRK5 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human GRK5
Pro463-Ser590
Accession # P34947
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Knockout Validated
GRK5 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in GRK5 knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human/Mouse/Rat GRK5 antibody by Western Blot. View Larger

Detection of Human/Mouse/Rat GRK5 by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line, Rat-2 rat embryonic fibroblast cell line, and BaF3 mouse pro-B cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat GRK5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4539) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GRK5 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated Western Blot Shows Human GRK5 Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human GRK5 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and GRK5 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat GRK5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4539) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GRK5 at approximately 65 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GRK5

G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK5 is a member of the GRK4 subfamily, and is expressed in a wide variety of tissues.

Long Name
G protein-coupled Receptor Kinase 5
Entrez Gene IDs
2869 (Human); 14773 (Mouse); 59075 (Rat)
Alternate Names
EC 2.7.11; EC 2.7.11.16; FLJ39780; G protein-coupled receptor kinase 5; G protein-coupled receptor kinase GRK5; GPRK5; GPRK5FP2025; GRK5

Product Datasheets

Citations for Human/Mouse/Rat GRK5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Dissecting the roles of GRK2 and GRK3 in ?-opioid receptor internalization and &beta-arrestin2 recruitment using CRISPR/Cas9-edited HEK293 cells
    Authors: TC Møller, MF Pedersen, JR van Senten, SD Seiersen, JM Mathiesen, M Bouvier, H Bräuner-Os
    Sci Rep, 2020;10(1):17395.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Distinct cellular and subcellular distributions of G protein-coupled receptor kinase and arrestin isoforms in the striatum.
    Authors: Bychkov, Evgeny, Zurkovsky, Lilia, Garret, Mika B, Ahmed, Mohamed, Gurevich, Eugenia
    PLoS ONE, 2012;7(11):e48912.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. GRK5 promotes F-actin bundling and targets bundles to membrane structures to control neuronal morphogenesis.
    Authors: Chen Y, Wang F, Long H
    J. Cell Biol., 2011;194(6):905-20.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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