Human/Mouse/Rat GRK5 Antibody Summary
Accession # P34947
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human/Mouse/Rat GRK5 by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line, Rat-2 rat embryonic fibroblast cell line, and BaF3 mouse pro-B cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat GRK5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4539) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GRK5 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human GRK5 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and GRK5 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat GRK5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4539) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GRK5 at approximately 65 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK5 is a member of the GRK4 subfamily, and is expressed in a wide variety of tissues.
Citations for Human/Mouse/Rat GRK5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Dissecting the roles of GRK2 and GRK3 in ?-opioid receptor internalization and &beta-arrestin2 recruitment using CRISPR/Cas9-edited HEK293 cells
Authors: TC Møller, MF Pedersen, JR van Senten, SD Seiersen, JM Mathiesen, M Bouvier, H Bräuner-Os
Sci Rep, 2020;10(1):17395.
Sample Types: Cell Lysates
Applications: Western Blot
Distinct cellular and subcellular distributions of G protein-coupled receptor kinase and arrestin isoforms in the striatum.
Authors: Bychkov, Evgeny, Zurkovsky, Lilia, Garret, Mika B, Ahmed, Mohamed, Gurevich, Eugenia
PLoS ONE, 2012;7(11):e48912.
Sample Types: Tissue Homogenates
Applications: Western Blot
GRK5 promotes F-actin bundling and targets bundles to membrane structures to control neuronal morphogenesis.
Authors: Chen Y, Wang F, Long H
J. Cell Biol., 2011;194(6):905-20.
Sample Types: Whole Cells
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