Human/Mouse/Rat HIF-1 alpha Antibody AF1935: R&D Systems

Human/Mouse/Rat HIF-1 alpha Antibody

(16 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse and rat HIF-1 alpha.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human HIF-1 alpha
    Arg575-Asn826
    Accession # Q16665.1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Immunoprecipitation
    2 µg/500 µg cell lysate
    MCF‑7 human breast cancer cell line treated with CoCl2, see our available Western blot detection antibodies
  • Chromatin Immunoprecipitation (ChIP)
    5 µg/5 x 106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human HIF‑1 alpha by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line untreated (-) or treated (+) with 150 μM CoCl2 for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat HIF‑1 alpha Antigen-affinity Purified Polyclonal Antibody, followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIF‑1 alpha at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Chromatin Immunoprecipitation (ChIP)

Detection of HIF‑1 alpha -regulated Genes by Chromatin Immunoprecipitation. Mouse primary kidney cells treated with 150 μM CoCl2 for overnight were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HIF‑1 alpha /DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse/Rat HIF‑1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1935) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The epo promoter was detected by standard PCR.

Detection of Human HIF‑1 alpha by Simple WesternTM. Simple Western lane view shows lysates of A549 human lung carcinoma cell line untreated (-) or treated (+) with Hypoxia (1% O2), loaded at 0.2 mg/mL. A specific band was detected for HIF‑1 alpha at approximately 115 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat HIF‑1 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1935) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HIF-1 alpha

The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha ) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.

  • Long Name:
    Hypoxia-inducible Transcription Factor 1 alpha
  • Entrez Gene IDs:
    3091 (Human); 15251 (Mouse); 29560 (Rat)
  • Alternate Names:
    ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; BHLHE78; Class E basic helix-loop-helix protein 78; HIF 1A; HIF1 alpha; HIF-1 alpha; HIF1A; HIF-1a; HIF-1-alpha; HIF1-alpha; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcriptionfactor); hypoxia-inducible factor 1-alpha; Member of PAS protein 1; member of PAS superfamily 1; MOP1HIF1-ALPHA; PAS domain-containing protein 8; PASD8alpha subunit (basic helix-loop-helix transcriptionfactor)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Motor neurons control blood vessel patterning in the developing spinal cord
    Authors: P Himmels, I Paredes, H Adler, A Karakatsan, R Luck, HH Marti, O Ermakova, E Rempel, ET Stoeckli, C Ruiz de Al
    Nat Commun, 2017;8(0):14583.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Metabolic reprogramming is associated with flavopiridol resistance in prostate cancer DU145 cells
    Authors: X Li, J Lu, Q Kan, X Li, Q Fan, Y Li, R Huang, A Slipicevic, HP Dong, L Eide, J Wang, H Zhang, V Berge, MA Goscinski, G Kvalheim, JM Nesland, Z Suo
    Sci Rep, 2017;7(1):5081.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Forkhead Box O3 (FoxO3) Regulates Kidney Tubular Autophagy Following Urinary Tract Obstruction
    Authors: L Li, R Zviti, C Ha, ZV Wang, JA Hill, F Lin
    J. Biol. Chem., 2017;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  4. Endothelial adenosine A2a receptor-mediated glycolysis is essential for pathological retinal angiogenesis
    Authors: Z Liu, S Yan, J Wang, Y Xu, Y Wang, S Zhang, X Xu, Q Yang, X Zeng, Y Zhou, X Gu, S Lu, Z Fu, DJ Fulton, NL Weintraub, RB Caldwell, W Zhang, C Wu, XL Liu, JF Chen, A Ahmad, I Kaddour-Dj, M Al-Shabraw, Q Li, X Jiang, Y Sun, A Sodhi, L Smith, M Hong, Y Huo
    Nat Commun, 2017;8(1):584.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. HEREGULIN/ErbB3 SIGNALING ENHANCES CXCR4-DRIVEN Rac1 ACTIVATION AND BREAST CANCER CELL MOTILITY VIA HIF-1?
    Mol Cell Biol, 2016;0(0):.
    Species: Human
    Sample Type: Chromatin
    Application: ChIP Assay
  6. Identification of ?-Dystrobrevin as a Direct Target of miR-143: Involvement in Early Stages of Neural Differentiation
    PLoS ONE, 2016;11(5):e0156325.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Stress-resistant Translation of Cathepsin L mRNA in Breast Cancer Progression.
    Authors: Tholen M, Wolanski J, Stolze B, Chiabudini M, Gajda M, Bronsert P, Stickeler E, Rospert S, Reinheckel T
    J Biol Chem, 2015;290(25):15758-69.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  8. High Glucose Up-regulates ADAM17 through HIF-1alpha in Mesangial Cells.
    Authors: Li R, Uttarwar L, Gao B, Charbonneau M, Shi Y, Chan J, Dubois C, Krepinsky J
    J Biol Chem, 2015;290(35):21603-14.
    Species: Rat
    Sample Type: Cell Lysates
    Application: ChIP
  9. Warburg-like Glycolysis and Lactate Shuttle in Mouse Decidua during Early Pregnancy.
    Authors: Zuo R, Gu X, Qi Q, Wang T, Zhao X, Liu J, Yang Z
    J Biol Chem, 2015;290(35):21280-91.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  10. Myeloid cell leukemia-1 (Mc1-1) is a candidate target gene of hypoxia-inducible factor-1 (HIF-1) in the testis.
    Authors: Palladino M, Shah A, Tyson R, Horvath J, Dugan C, Karpodinis M
    Reprod Biol Endocrinol, 2012;10(0):104.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: ChIP
  11. The hypoxic microenvironment upgrades stem-like properties of ovarian cancer cells.
    Authors: Liang D, Ma Y, Liu J, Trope CG, Holm R, Nesland JM, Suo Z
    BMC Cancer, 2012;12(0):201.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  12. Development of an in vitro model of myotube ischemia.
    Authors: Joshi D, Patel H, Baker DM, Shiwen X, Abraham DJ, Tsui JC
    Lab. Invest., 2011;91(8):1241-52.
    Species: Mouse
    Sample Type: Whole Cells
    Application: WB
  13. HIF-1-mediated up-regulation of cardiotrophin-1 is involved in the survival response of cardiomyocytes to hypoxia.
    Authors: Robador PA, San Jose G, Rodriguez C, Guadall A, Moreno MU, Beaumont J, Fortuno A, Diez J, Martinez-Gonzalez J, Zalba G
    Cardiovasc. Res., 2011;92(2):247-55.
    Species: Human
    Sample Type: Cell Lysates
    Application: ChIP
  14. HER-2 signaling, acquisition of growth factor independence, and regulation of biological networks associated with cell transformation.
    Authors: Bollig-Fischer A, Dziubinski M, Boyer A, Haddad R, Giroux CN, Ethier SP
    Cancer Res., 2010;70(20):7862-73.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  15. Hypoxia induces leptin gene expression and secretion in human preadipocytes: differential effects of hypoxia on adipokine expression by preadipocytes.
    Authors: Wang B, Wood IS, Trayhurn P
    J. Endocrinol., 2008;198(1):127-34.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  16. The new tumor-suppressor gene inhibitor of growth family member 4 (ING4) regulates the production of proangiogenic molecules by myeloma cells and suppresses hypoxia-inducible factor-1 alpha (HIF-1alpha) activity: involvement in myeloma-induced angiogenesis.
    Authors: Colla S, Tagliaferri S, Morandi F, Lunghi P, Donofrio G, Martorana D, Mancini C, Lazzaretti M, Mazzera L, Ravanetti L, Bonomini S, Ferrari L, Miranda C, Ladetto M, Neri TM, Neri A, Greco A, Mangoni M, Bonati A, Rizzoli V, Giuliani N
    Blood, 2007;110(13):4464-75.
    Species: Human
    Sample Type: Nuclear Extract
    Application: WB
Expand to show all 16 Citations
ExactaChIP Chromatin Immunoprecipitation Kits
Description Application Cat# Citations Images  

ExactaChIP Chromatin IP Buffer Panel

ChIP ECP001 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG HRP-conjugated Antibody

WB HAF017 15  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Antibody

WB AF109 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG VisUCyte HRP Polymer

IHC VC004  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human/Mouse/Rat HIF-1 alpha Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Associated Pathways

Feedback

Customer Information