Human/Mouse/Rat HSPH1 Antibody

Catalog #: AF4029 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4029
AF4029-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat HSPH1 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat HSPH1. This antibody is predicted to react with HSPH1 alpha and beta isoforms based on sequence identity of the immunogen.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant rat HSPH1
Val595-Asp733
Accession # Q66HA8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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HSPH1 in Rat Brain. HSPH1 was detected in perfusion fixed frozen sections of rat brain (cerebellum) using 5 µg/mL Human/Mouse/Rat HSPH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4029) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western Blot

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Detection of Human/Mouse/Rat HSPH1 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, DA3 mouse myeloma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.1 µg/mL of Human/Mouse/Rat HSPH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4029) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HSPH1 at approximately 105-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSPH1

The heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Proteins are ubiquitously expressed in all organisms. They are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation. Heat shock protein H1 (HSPH1), also known as HSP105 and HSP110, exists as two isoforms, alpha and beta. HSPH1 alpha consists of 858 amino acids whereas HSPH1 beta has 814 amino acids. alpha and beta have calculated molecular weights of 96.4 kDa and 92 kDa, respectively, but an apparent molecular weight of 105‑110 kDa in SDS‑PAGE. HSPH1 acts as a chaperone to prevent thermal aggregation in mammalian cells. HSPH1 alpha and beta isoforms share 94% identity. Rat HSPH1 has 92% and 97% identity to human and mouse HSPH1, respectively.

Long Name

Heat Shock Protein H1

Entrez Gene IDs

10808 (Human); 288444 (Rat)

Alternate Names

Antigen NY-CO-25; heat shock 105kD beta; heat shock 105kDa protein 1; heat shock 105kDa/110kDa protein 1; Heat shock 110 kDa protein; heat shock protein 105 kDa; HSP105a; HSP105B; HSP105heat shock 105kD alpha; HSP110; HSPH1; KIAA0201DKFZp686M05240; NY-CO-25